SCIENCE@home

Development and Characterization of a Clinically Compliant Xeno‐Free Culture Medium in Good Manufacturing Practice for Human Multipotent Mesenchymal Stem Cells

L. Chase,Sufang Yang,V. Zachar,Zheng Yang,U. Lakshmipathy,Jolene A. Bradford,Shayne E. Boucher,M. Vemuri

Published 2012 in Stem Cells Translational Medicine

ABSTRACT

Human multipotent mesenchymal stem cell (MSC) therapies are currently being tested in clinical trials for Crohn's disease, multiple sclerosis, graft‐versus‐host disease, type 1 diabetes, bone fractures, cartilage damage, and cardiac diseases. Despite remarkable progress in clinical trials, most applications still use traditional culture media containing fetal bovine serum or serum‐free media that contain serum albumin, insulin, and transferrin. The ill‐defined and variable nature of traditional culture media remains a challenge and has created a need for better defined xeno‐free culture media to meet the regulatory and long‐term safety requirements for cell‐based therapies. We developed and tested a serum‐free and xeno‐free culture medium (SFM‐XF) using human bone marrow‐ and adipose‐derived MSCs by investigating primary cell isolation, multiple passage expansion, mesoderm differentiation, cellular phenotype, and gene expression analysis, which are critical for complying with translation to cell therapy. Human MSCs expanded in SFM‐XF showed continual propagation, with an expected phenotype and differentiation potential to adipogenic, chondrogenic, and osteogenic lineages similar to that of MSCs expanded in traditional serum‐containing culture medium (SCM). To monitor global gene expression, the transcriptomes of bone marrow‐derived MSCs expanded in SFM‐XF and SCM were compared, revealing relatively similar expression profiles. In addition, the SFM‐XF supported the isolation and propagation of human MSCs from primary human marrow aspirates, ensuring that these methods and reagents are compatible for translation to therapy. The SFM‐XF culture system allows better expansion and multipotentiality of MSCs and serves as a preferred alternative to serum‐containing media for the production of large scale, functionally competent MSCs for future clinical applications.

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-37 of 37 references · Page 1 of 1

CITED BY

Showing 1-100 of 113 citing papers · Page 1 of 2

12
Next