Phenotypic and transcriptional analysis of the antimicrobial effect of lactic acid bacteria on carbapenem-resistant Acinetobacter baumannii: Lacticaseibacillus rhamnosus CRL 2244 an alternative strategy to overcome resistance?”

Abstract Carbapenem-resistant Acinetobacter baumannii (CRAB) is a recognized nosocomial pathogen with limited antibiotic treatment options. Lactic acid bacteria (LAB) constitute a promising therapeutic alternative. Here we studied the antibacterial properties of a collection of LAB strains using phenotypic and transcriptomic analysis against A. baumannii clinical strains. One strain, Lacticaseibacillus rhamnosus CRL 2244, demonstrated a potent inhibitory capacity on A. baumannii with a significant killing activity. Scanning electron microscopy images showed changes in the morphology of A. baumannii with an increased formation of outer membrane vesicles. Significant changes in the expression levels of a wide variety of genes were also observed. Interestingly, most of the modified genes were involved in a metabolic pathway known to be associated with the survival of A. baumannii . The paa operon, Hut system, and fatty acid degradation were some of the pathways that were induced. The analysis reveals the impact of Lcb. rhamnosus CRL 2244 on A. baumannii response, resulting in bacterial stress and subsequent cell death. These findings highlight the antibacterial properties of Lcb. rhamnosus CRL 2244 and its potential as an alternative or complementary strategy for treating infections. Further exploration and development of LAB as a treatment option could provide valuable alternatives for combating CRAB infections.

• Publication year

  2023

• Venue

  Research Square

• Publication date

  2023-07-21

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.21203/rs.3.rs-3151881/v1PMID 37503046PMCID 10371144
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Probiotic Lactobacillus Species and Their Biosurfactants Eliminate Acinetobacter baumannii Biofilm in Various Manners

  2023cited by this paper
• Characterizing the role of phosphatidylglycerol-phosphate phosphatases in Acinetobacter baumannii cell envelope biogenesis and antibiotic resistance

  2022cited by this paper
• The Phenylacetic Acid Catabolic Pathway Regulates Antibiotic and Oxidative Stress Responses in Acinetobacter

  2022cited by this paper
• The Staphylococcus aureus CidA and LrgA Proteins Are Functional Holins Involved in the Transport of By-Products of Carbohydrate Metabolism

  2022cited by this paper
• Inhibition of a spoilage exopolysaccharide producer by bioprotective extracts from Lactobacillus acidophilus CRL641 and Latilactobacillus curvatus CRL705 in vacuum-packaged refrigerated meat discs.

  2021cited by this paper
• Antibiotic-Resistant Gram-negative Bacteria Carriage in Healthcare Workers Working in an Intensive Care Unit

  2021cited by this paper
• Risk factors for nosocomial rectal colonization with carbapenem-resistant Acinetobacter baumannii in hospital: a matched case–control study

  2021cited by this paper
• Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii

  2021cited by this paper
• Screening of aroma-producing lactic acid bacteria and their application in improving the aromatic profile of yogurt.

  2020cited by this paper
• A novel antibacterial compound produced by Lactobacillus plantarum LJR13 isolated from rumen liquor of goat effectively controls multi-drug resistant human pathogens.

  2020cited by this paper
• Antimicrobial Resistance of Serial Isolates of Acinetobacter baumannii Colonizing the Rectum of Adult Intensive Care Unit Patients in a Teaching Hospital in Kuwait

  2020cited by this paper
• Distinct Mechanisms of Dissemination of NDM-1 Metallo-β-Lactamase in Acinetobacter Species in Argentina

  2020cited by this paper
• Histidine Utilization Is a Critical Determinant of Acinetobacter Pathogenesis

  2020cited by this paper
• Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace

  2020cited by this paper
• Selection of Potential Probiotic Bacteria from Exclusively Breastfed Infant Faeces with Antagonistic Activity Against Multidrug-Resistant ESKAPE Pathogens

  2020cited by this paper
• Invited review: Starter lactic acid bacteria survival in cheese: New perspectives on cheese microbiology.

  2020cited by this paper
• Characterization of ST25 blaNDM-1 producing Acinetobacter spp. strains leading the increase in NDM-1 emergence in Argentina.

  2020cited by this paper
• Beneficial Effects of Probiotic Consumption on the Immune System

  2019cited by this paper
• Screening of aroma-producing lactic acid bacteria and their application in improving the aromatic profile of yogurt.

  2019cited by this paper
• Topical Delivery of Lactobacillus Culture Supernatant Increases Survival and Wound Resolution in Traumatic Acinetobacter baumannii Infections

  2019cited by this paper
• Characterization of LrgAB as a stationary phase-specific pyruvate uptake system in Streptococcus mutans

  2019cited by this paper
• Human Pleural Fluid Elicits Pyruvate and Phenylalanine Metabolism in Acinetobacter baumannii to Enhance Cytotoxicity and Immune Evasion

  2019cited by this paper
• Lactobacillus casei BL23 modulates the innate immune response in Staphylococcus aureus-stimulated bovine mammary epithelial cells.

  2018cited by this paper
• Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii

  2018cited by this paper
• Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity

  2018cited by this paper
• Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

  2017cited by this paper
• The effect of immune modulation of Streptococcus constellatus SC10 strain upon Acinetobactor baumannii infection.

  2017cited by this paper
• Probiotics and antibiotic-associated diarrhea in children: A review and new evidence on Lactobacillus rhamnosus GG during and after antibiotic treatment.

  2017cited by this paper
• Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034

  2017cited by this paper
• Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis

  2016cited by this paper
• The LysR‐type transcriptional regulator, CidR, regulates stationary phase cell death in Staphylococcus aureus

  2016cited by this paper
• Probiotic Potential of Lactobacillus Strains with Antimicrobial Activity against Some Human Pathogenic Strains

  2014cited by this paper
• A global virulence regulator in Acinetobacter baumannii and its control of the phenylacetic acid catabolic pathway.

  2014cited by this paper
• Antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens

  2014cited by this paper
• Preliminary Evaluation of Probiotic Properties of Lactobacillus Strains Isolated from Sardinian Dairy Products

  2014cited by this paper
• Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri

  2014cited by this paper
• AB5075, a Highly Virulent Isolate of Acinetobacter baumannii, as a Model Strain for the Evaluation of Pathogenesis and Antimicrobial Treatments

  2014cited by this paper
• Lactobacillus reuteri CRL1101 beneficially modulate lipopolysaccharide-mediated inflammatory response in a mouse model of endotoxic shock

  2013cited by this paper
• Mannitol production by heterofermentative Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in free and controlled pH batch fermentations

  2012cited by this paper
• Inhibitory activity of fermented milk with Lactobacillus casei strain Shirota against common multidrug-resistant bacteria causing hospital-acquired infections.

  2012cited by this paper
• Genomic Analysis of Acinetobacter baumannii A118 by Comparison of Optical Maps: Identification of Structures Related to Its Susceptibility Phenotype

  2011cited by this paper
• Lactic Acid Bacteria

  2011cited by this paper
• Therapeutic effect of Streptococcus thermophilus CRL 1190-fermented milk on chronic gastritis.

  2010cited by this paper
• Neutrophil unsaturated fatty acid release by GM-CSF is impaired in cystic fibrosis

  2010cited by this paper
• Prevention of chronic gastritis by fermented milks made with exopolysaccharide-producing Streptococcus thermophilus strains.

  2009cited by this paper
• Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

  2008cited by this paper
• A Functional Phenylacetic Acid Catabolic Pathway Is Required for Full Pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans Host Model

  2008cited by this paper
• Analysis of Antibiotic Resistance Genes in Multidrug-Resistant Acinetobacter sp. Isolates from Military and Civilian Patients Treated at the Walter Reed Army Medical Center

  2006cited by this paper
• Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications.

  2006cited by this paper
• Comparative Genomics of Multidrug Resistance in Acinetobacter baumannii

  2006cited by this paper
• A Novel Functional Soy-based Food Fermented by Lactic Acid Bacteria: Effect of Heat Treatment

  2004cited by this paper
• Control of Listeria monocytogenes in ground beef by 'Lactocin 705', a bacteriocin produced by Lactobacillus casei CRL 705).

  1996cited by this paper
• Release and metabolism of arachidonic acid in human neutrophils.

  1981cited by this paper

• In Silico Identification and Molecular Characterization of Lentilactobacillus hilgardii Antimicrobial Peptides with Activity Against Carbapenem-Resistant Acinetobacter baumannii

  2025cites this paper