BackgroundTaste receptor cells are responsible for transducing chemical stimuli into electrical signals that lead to the sense of taste. An important second messenger in taste transduction is IP3, which is involved in both bitter and sweet transduction pathways. Several components of the bitter transduction pathway have been identified, including the T2R/TRB taste receptors, phospholipase C β2, and the G protein subunits α-gustducin, β3, and γ13. However, the identity of the IP3 receptor subtype in this pathway is not known. In the present study we used immunocytochemistry on rodent taste tissue to identify the IP3 receptors expressed in taste cells and to examine taste bud expression patterns for IP3R3.ResultsAntibodies against Type I, II, and III IP3 receptors were tested on sections of rat and mouse circumvallate papillae. Robust cytoplasmic labeling for the Type III IP3 receptor (IP3R3) was found in a large subset of taste cells in both species. In contrast, little or no immunoreactivity was seen with antibodies against the Type I or Type II IP3 receptors. To investigate the potential role of IP3R3 in bitter taste transduction, we used double-label immunocytochemistry to determine whether IP3R3 is expressed in the same subset of cells expressing other bitter signaling components. IP3R3 immunoreactive taste cells were also immunoreactive for PLCβ2 and γ13. Alpha-gustducin immunoreactivity was present in a subset of IP3R3, PLCβ2, and γ13 positive cells.ConclusionsIP3R3 is the dominant form of the IP3 receptor expressed in taste cells and our data suggest it plays an important role in bitter taste transduction.
Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction
T. Clapp,Leslie M. Stone,R. Margolskee,S. Kinnamon
Published 2001 in BMC Neuroscience
ABSTRACT
PUBLICATION RECORD
- Publication year
2001
- Venue
BMC Neuroscience
- Publication date
2001-04-23
- Fields of study
Biology, Medicine
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- alpha-gustducin
A taste-associated G protein alpha subunit examined for overlap with IP3R3-positive cells.
Aliases: α-gustducin
- bitter taste transduction
The signaling pathway in taste cells that converts bitter chemical stimuli into intracellular responses.
Aliases: bitter transduction pathway
- circumvallate papillae
Tongue papillae containing taste buds that were sectioned from rat and mouse tissue for the staining experiments.
- g protein gamma13
A G protein gamma subunit examined for co-expression with other bitter taste signaling components in taste cells.
Aliases: γ13
- immunocytochemistry
An antibody-based staining method used here to localize IP3 receptor and signaling protein expression in taste tissue sections.
- plcβ2
A phospholipase C beta isoform examined as part of the bitter taste signaling machinery in taste cells.
- type iii ip3 receptor (ip3r3)
The type III inositol 1,4,5-trisphosphate receptor subtype examined for expression in rodent taste cells.
Aliases: Type III IP3 receptor, IP3R3
- type ii ip3 receptor
The type II inositol 1,4,5-trisphosphate receptor subtype tested for immunoreactivity in taste tissue sections.
- type i ip3 receptor
The type I inositol 1,4,5-trisphosphate receptor subtype tested for immunoreactivity in taste tissue sections.
REFERENCES
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