Design of an effective sgRNA for CRISPR/Cas9 knock-ins in polyploid Synechocystis sp. PCC 6803

No abstract is available for this paper.

• Publication year

  2023

• Venue

  bioRxiv

• Publication date

  2023-09-27

• Fields of study

  Biology, Engineering, Environmental Science

• Identifiers
  DOI 10.1101/2023.09.25.559380
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Off-target effects in CRISPR/Cas9 gene editing

  2023cited by this paper
• Characterizing off-target effects of genome editors

  2023cited by this paper
• Rarely acquired type II-A CRISPR-Cas spacers mediate anti-viral immunity through the targeting of a non-canonical PAM sequence

  2023influential reference
• Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins

  2022cited by this paper
• Exploring and engineering PAM-diverse Streptococci Cas9 for PAM-directed bifunctional and titratable gene control in bacteria.

  2022cited by this paper
• Photoautotrophic production of p-Coumaric acid using genetically engineered Synechocystis sp. Pasteur Culture Collection 6803

  2021cited by this paper
• Orthogonal CRISPR-Cas tools for genome editing, inhibition, and CRISPR recording in zebrafish embryos.

  2021cited by this paper
• Escherichia coli AraJ boosts utilization of arabinose in metabolically engineered cyanobacterium Synechocystis sp. PCC 6803

  2021cited by this paper
• Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

  2020cited by this paper
• Outer membrane-deprived cyanobacteria liberate periplasmic and thylakoid luminal components that support the growth of heterotrophs

  2020cited by this paper
• An Improved Natural Transformation Protocol for the Cyanobacterium Synechocystis sp. PCC 6803

  2020cited by this paper
• Construction of Fully Segregated Genomic Libraries in Polyploid Organisms Such as Synechocystis sp. PCC 6803

  2020cited by this paper
• Combining Random Mutagenesis and Metabolic Engineering for Enhanced Tryptophan Production in Synechocystis sp. Strain PCC 6803

  2020cited by this paper
• Improving Synechocystis sp. PCC 6803 as a model organism

  2020cited by this paper
• Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit.

  2019influential reference
• Progress and challenges in engineering cyanobacteria as chassis for light‐driven biotechnology

  2019cited by this paper
• Cyanobacterial Cell Factories for Improved Carotenoid Biosynthesis through a Synthetic Biology Approach

  2019cited by this paper
• High cetane renewable diesel fuels prepared from bio-based methyl ketones and diols

  2018cited by this paper
• Metabolic engineering of Synechocystis PCC 6803 with the aim to enhance isobutanol/1-butanol production using CRISPR interference

  2017cited by this paper
• NOT Gate Genetic Circuits to Control Gene Expression in Cyanobacteria.

  2017cited by this paper
• Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors

  2017cited by this paper
• Refined sgRNA efficacy prediction improves large- and small-scale CRISPR–Cas9 applications

  2017cited by this paper
• Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering.

  2016cited by this paper
• Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis

  2016cited by this paper
• CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production.

  2016cited by this paper
• Cyanobacterial metabolic engineering for biofuel and chemical production.

  2016cited by this paper
• Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9

  2016cited by this paper
• Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803

  2016cited by this paper
• The ploidy level of Synechocystis sp. PCC 6803 is highly variable and is influenced by growth phase and by chemical and physical external parameters.

  2016cited by this paper
• CRISPR technologies for bacterial systems: Current achievements and future directions.

  2016cited by this paper
• Cyanobacterial chassis engineering for enhancing production of biofuels and chemicals

  2016cited by this paper
• rNA-guided editing of bacterial genomes using crisPr-cas systems

  2016cited by this paper
• Genome engineering using CRISPR-Cas9 system.

  2015cited by this paper
• Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide

  2015cited by this paper
• Improving a Synechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites

  2015cited by this paper
• Genome Engineering in Cyanobacteria: Where We Are and Where We Need To Go.

  2015influential reference
• Development and applications of CRISPR-Cas9 for genome engineering.

  2014cited by this paper
• Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair

  2014cited by this paper
• Efficient gene targeting in zebrafish mediated by a zebrafish-codon-optimized cas9 and evaluation of off-targeting effect.

  2014cited by this paper
• CRISPR-Cas systems: Prokaryotes upgrade to adaptive immunity.

  2014influential reference
• The new frontier of genome engineering with CRISPR-Cas9

  2014cited by this paper
• CasOT: a genome-wide Cas9/gRNA off-target searching tool

  2014cited by this paper
• DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

  2014cited by this paper
• Multiplex Genome Engineering Using CRISPR/Cas Systems

  2013cited by this paper
• A novel counter‐selection method for markerless genetic modification in Synechocystis sp. PCC 6803

  2013cited by this paper
• An Organic Acid Based Counter Selection System for Cyanobacteria

  2013cited by this paper
• Synthetic biology of cyanobacteria: unique challenges and opportunities

  2013cited by this paper
• Wide-dynamic-range promoters engineered for cyanobacteria

  2013cited by this paper
• Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

  2013cited by this paper
• Bacterial natural transformation by highly fragmented and damaged DNA

  2013cited by this paper
• RNA-programmed genome editing in human cells

  2013cited by this paper
• CRISPR interference (CRISPRi) for sequence-specific control of gene expression

  2013cited by this paper
• A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

  2012cited by this paper
• Quantification of Ploidy in Proteobacteria Revealed the Existence of Monoploid, (Mero-)Oligoploid and Polyploid Species

  2011cited by this paper
• The production of the sesquiterpene β-caryophyllene in a transgenic strain of the cyanobacterium Synechocystis.

  2011cited by this paper
• CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

  2011cited by this paper
• ZipN, an FtsA‐like orchestrator of divisome assembly in the model cyanobacterium Synechocystis PCC6803

  2009cited by this paper
• Synechocystis sp. PCC 6803 — a useful tool in the study of the genetics of cyanobacteria

  2004cited by this paper

• No citing papers are available for this paper.