Pre-oligodendrocytes from adult human CNS

CNS remyelination and functional recovery often occur after experimental demyelination in adult rodents. This has been attributed to the ability of mature oligodendrocytes and/or their precursor cells to divide and regenerate in response to signals in demyelinating lesions. To determine whether oligodendrocyte precursor cells exist in the adult human CNS, we have cultured white matter from patients undergoing partial temporal lobe resection for intractable epilepsy. These cultures contained a population of process-bearing cells that expressed antigens recognized by the O4 monoclonal antibody, but these cells did not express galactocerebroside (a marker for oligodendrocytes), glial fibrillary acidic protein (a marker for astrocytes), or vimentin. Selective elimination of O4-positive (O4+) cells by complement-mediated lysis resulted in inhibition of oligodendrocyte development in vitro. Since O4+ cells have an antigenic phenotype reminiscent of the rat adult oligodendrocyte-type 2 astrocyte progenitor and appear to develop into oligodendrocytes rather than type 2 astrocytes with time in culture, we call them “pre-oligodendrocytes.” Neither pre-oligodendrocytes nor oligodendrocytes incorporated 3H- thymidine in response to rat astrocyte-conditioned medium, platelet- derived growth factor, insulin-like growth factor (IGF-1), or basic fibroblast growth factor (bFGF). However, IGF-1 increased the relative abundance of oligodendrocytes to pre-oligodendrocytes, while bFGF had the opposite effect. Cells with the antigenic phenotype of pre- oligodendrocytes were also identified in tissue prints of adult human white matter. We propose that, in human demyelinating diseases such as multiple sclerosis, pre-oligodendrocytes may divide and/or migrate in response to signals present in demyelinated lesions and thus facilitate remyelination.

• Publication year

  1992

• Venue

  Journal of Neuroscience

• Publication date

  1992-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1523/JNEUROSCI.12-04-01538.1992PMID 1556607PMCID PMC6575800
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• O‐2A glial progenitors from mature brain respond to CNS neuronal cell line‐derived growth factors

  1991cited by this paper
• Platelet‐derived growth factor is mitogenic for O‐2Aadult progenitor cells

  1991cited by this paper
• The origin of remyelinating cells in the adult central nervous system: The role of the mature oligodendrocyte

  1991cited by this paper
• Ion channel expression by white matter glia: the type-1 astrocyte.

  1990cited by this paper
• Two proliferative stages of the oligodendrocyte lineage (A2B5+O4- and O4+GalC-) under different mitogenic control.

  1990cited by this paper
• Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly.

  1990cited by this paper
• The cellular and molecular events of central nervous system remyelination

  1990cited by this paper
• In vitro analysis of the oligodendrocyte lineage in mice during demyelination and remyelination

  1990cited by this paper
• Coexistence of perinatal and adult forms of a glial progenitor cell during development of the rat optic nerve.

  1990cited by this paper
• Type 1 astrocytes and oligodendrocyte‐type 2 astrocyte glial progenitors migrate toward distinct molecules

  1990influential reference
• FGF modulates the PDGF-driven pathway of oligodendrocyte development.

  1990cited by this paper
• Oligodendrocyte-type 2 astrocyte (O-2A) progenitor cells from neonatal and adult rat optic nerve differ in their responsiveness to platelet-derived growth factor.

  1990cited by this paper
• Cooperation between two growth factors promotes extended self-renewal and inhibits differentiation of oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells.

  1990influential reference
• Neurobiology of human oligodendrocytes in culture

  1990cited by this paper
• Cells positive for the O4 surface antigen isolated by cell sorting are able to differentiate into astrocytes or oligodendrocytes.

  1989influential reference
• Identification of an adult-specific glial progenitor cell.

  1989influential reference
• Multiple and novel specificities of monoclonal antibodies O1, O4, and R‐mAb used in the analysis of oligodendrocyte development

  1989cited by this paper
• Oligodendrocytes and oligodendrocyte/type-2 astrocyte progenitor cells of adult rats are specifically susceptible to the lytic effects of complement in absence of antibody.

  1989cited by this paper
• In vivo analysis of glial cell phenotypes during a viral demyelinating disease in mice

  1989cited by this paper
• Oligodendrocyte progenitors isolated directly from developing telencephalon at a specific phenotypic stage: myelinogenic potential in a defined environment.

  1989cited by this paper
• Radioautographic evidence for slow astrocyte turnover and modest oligodendrocyte production in the corpus callosum of adult mice infused with 3H‐thymidine

  1988cited by this paper
• Development of macroglial cells in rat cerebellum. I. Use of antibodies to follow earlyin vivo development and migration of oligodendrocytes

  1988cited by this paper
• Growth factors for human glial cells in culture

  1988influential reference
• Platelet-derived growth factor from astrocytes drives the clock that times oligodendrocyte development in culture

  1988cited by this paper
• Induction of oligodendrocyte proliferation and remyelination after chronic demyelination. Relevance to multiple sclerosis.

  1988cited by this paper
• On the immunopathology of plaque development and repair in multiple sclerosis.

  1988cited by this paper
• Insulin‐like growth factor I promotes cell proliferation and oligodendroglial commitment in rat glial progenitor cells developing in vitro

  1988cited by this paper
• Differentiation of cerebellar bipotential glial preecurosrs into oligodendrocytes in primary culture: Development profile of surface antigens and mitotic activity

  1987cited by this paper
• Characterization of a slowly proliferative cell along the oligodendrocyte differentiation pathway.

  1987cited by this paper
• Evidence that axons are mitogenic for oligodendrocytes isolated from adult animals

  1986influential reference
• Proliferating bipotential glial progenitor cells in adult rat optic nerve

  1986cited by this paper
• Emergence of three myelin proteins in oligodendrocytes cultured without neurons

  1986cited by this paper
• Insulin-like growth factor I/somatomedin C: a potent inducer of oligodendrocyte development.

  1986cited by this paper
• A quantitative immunohistochemical study of macroglial cell development in the rat optic nerve: in vivo evidence for two distinct astrocyte lineages.

  1985cited by this paper
• Mature oligodendrocytes. Division following experimental demyelination in adult animals.

  1984cited by this paper
• Continual Breakdown and Regeneration of Myelin in Progressive Multiple Sclerosis Plaques a

  1984cited by this paper
• The differentiation of oligodendrocytes in a serum-free hormone-supplemented medium.

  1984cited by this paper
• Long-term culture of human oligodendrocytes. Isolation, growth and identification.

  1983cited by this paper
• A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium

  1983cited by this paper
• Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside.

  1982influential reference
• Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system.

  1981cited by this paper
• An autoradiographic study of cellular proliferation in remyelination of the central nervous system.

  1979cited by this paper
• Cell-type-specific markers for distinguishing and studying neurons and the major classes of glial cells in culture.

  1979cited by this paper
• Thy-1 antigen on astrocytes in long-term cultures of rat central nervous system

  1979cited by this paper
• Monoclonal antibody to a plasma membrane antigen of neurons.

  1979cited by this paper
• Galactocerebroside is a specific cell-surface antigenic marker for oligodendrocytes in culture

  1978cited by this paper
• Physiological Basis of Conduction in Myelinated Nerve Fibers

  1977cited by this paper
• Pathophysiology of Conduction in Demyelinated Nerve Fibers

  1977cited by this paper
• Regeneration of oligodendroglia during recovery from demyelinating disease.

  1977cited by this paper
• Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence.

  1972cited by this paper
• Electron microscopic features of multiple sclerosis lesions.

  1965cited by this paper
• Oligodendrocytes and oligodendrocyte / type-2 astrocyte progenitor cells of adult rats are specifically susceptible to the Iytic effects of complement in absence of antibody ( multiple sclerosis / demyelination / remyelination )

  year unknowncited by this paper

• Remyelination as a therapeutic target in the treatment of multiple sclerosis

  2025cites this paper
• The Role of Oligodendrocytes in Alzheimer’s Disease Pathogenesis and Therapy

  2025cites this paper
• Age-dependent effects of metformin on human oligodendrocyte lineage cell ensheathment capacity

  2024cites this paper
• Myelination potential and injury susceptibility of grey versus white matter human oligodendrocytes.

  2024cites this paper
• Cells of the Central Nervous System: An Overview of Their Structure and Function.

  2023cites this paper
• Pyruvate Dehydrogenase-Dependent Metabolic Programming Affects the Oligodendrocyte Maturation and Remyelination

  2023cites this paper
• Newly Identified Deficiencies in the Multiple Sclerosis Central Nervous System and Their Impact on the Remyelination Failure

  2022cites this paper
• Glial progenitor cell-based repair of the dysmyelinated brain: Progression to the clinic.

  2021cites this paper
• Waning efficacy in a long-term AAV-mediated gene therapy study in the murine model of Krabbe disease

  2021cites this paper
• Hormonal Regulation of Oligodendrogenesis I: Effects across the Lifespan

  2021cites this paper
• Human Oligodendrocyte Myelination Potential; Relation to Age and Differentiation

  2021cites this paper
• The Wnt Effector TCF7l2 Promotes Oligodendroglial Differentiation by Repressing Autocrine BMP4-Mediated Signaling

  2020cites this paper
• The Impact of environmental and genetic insults on adult and developing brain oligodendrogenesis

  2020cites this paper
• Transplantation of induced neural stem cells (iNSCs) into chronically demyelinated corpus callosum ameliorates motor deficits

  2020cites this paper
• Hypothesis: Could Hepatitis B vaccine act as an immune adjuvant in glioblastoma? Clues to conduct further epidemiological analyses.

  2019cites this paper
• Distinct Function-Related Molecular Profile of Adult Human A2B5-Positive Pre-Oligodendrocytes Versus Mature Oligodendrocytes.

  2019cites this paper
• Heterogeneity and Proliferative and Differential Regulators of NG2-glia in Physiological and Pathological States.

  2019cites this paper
• Species differences in immune‐mediated CNS tissue injury and repair: A (neuro)inflammatory topic

  2019cites this paper
• Immunology of oligodendrocyte precursor cells in vivo and in vitro.

  2019cites this paper
• Organotypic CNS Slice Cultures as an in vitro Model for Damage and Repair in Multiple Sclerosis

  2018cites this paper
• Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes.

  2018cites this paper
• Characterization of oligodendrocyte lineage cell responses remote to the lesion site in a murine model of thoracic contusion spinal cord injury (SCI)

  2017cites this paper
• Progenitor cell-based treatment of glial disease.

  2017cites this paper
• Stem and Progenitor Cell-Based Therapy of the Central Nervous System: Hopes, Hype, and Wishful Thinking.

  2016cites this paper
• Cell Therapy for Pediatric Disorders of Glia

  2016cites this paper
• Role of differential heparan sulphate sulphation in Fgf/Erk signalling during mouse telencephalic development

  2016cites this paper
• Targeting human oligodendrocyte progenitors for myelin repair.

  2016cites this paper
• Glial progenitor cell-based treatment of the childhood leukodystrophies.

  2016influential citation
• How to make an oligodendrocyte

  2015cites this paper
• Identification of astrocytic factors that could play a role in myelination

  2015cites this paper
• NG 2-glia and their functions in the central nervous system

  2015cites this paper
• Remyelination in multiple sclerosis: Cellular mechanisms and novel therapeutic approaches

  2015cites this paper
• NG2‐glia and their functions in the central nervous system

  2015cites this paper
• Transcriptional analysis of the glial cell differentiation in the diseased and normal central nervous system

  2015influential citation
• Anti-Muscarinic Adjunct Therapy Accelerates Functional Human Oligodendrocyte Repair

  2015cites this paper
• Connexins-Mediated Glia Networking Impacts Myelination and Remyelination in the Central Nervous System

  2014cites this paper
• Distribution of Corticosteroid Receptors in Mature Oligodendrocytes and Oligodendrocyte Progenitors of the Adult Mouse Brain

  2014cites this paper
• Glial development: the crossroads of regeneration and repair in the CNS.

  2014cites this paper
• The PTEN inhibitor bisperoxovanadium enhances myelination by amplifying IGF‐1 signaling in rat and human oligodendrocyte progenitors

  2014cites this paper
• Temporal oligodendrocyte lineage progression: in vitro models of proliferation, differentiation and myelination.

  2014cites this paper
• Signalling Pathways that Inhibit the Capacity of Precursor Cells for Myelin Repair

  2013cites this paper
• Histone deacetylase activity is required for human oligodendrocyte progenitor differentiation

  2012cites this paper
• Neurodevelopmental effects of insulin-like growth factor signaling.

  2012cites this paper
• Rôle du gène Polycomb BMI1 dans le maintien et la radiorésistance des cellules souches cancéreuses

  2011cites this paper
• CD140a identifies a population of highly myelinogenic, migration-competent, and efficiently engrafting human oligodendrocyte progenitor cells

  2011cites this paper
• Targeting oligodendrocyte protection and remyelination in multiple sclerosis.

  2011cites this paper
• Redox signal transduction in oligodendrocytes differentiation: implications for multiple sclerosis pathogenesis

  2011cites this paper
• Promoting myelin repair and return of function in multiple sclerosis

  2011cites this paper
• Embryonic stem cell markers distinguishing cancer stem cells from normal human neuronal stem cell populations in malignant glioma patients.

  2010cites this paper
• Response of Human Oligodendrocyte Progenitors to Growth Factors and Axon Signals

  2010cites this paper
• Diffuse low-grade oligodendrogliomas extend beyond MRI-defined abnormalities

  2010cites this paper
• Protection of oligodendrocyte precursor cells by low doses of HSP90 inhibitors in cell culture.

  2010cites this paper
• Adult human brain cell culture for neuroscience research.

  2010cites this paper
• Myelin and Myelination

  2010cites this paper
• Robust Generation of Oligodendrocyte Progenitors from Human Neural Stem Cells and Engraftment in Experimental Demyelination Models in Mice

  2010cites this paper
• Characterization of oligodendrocyte lineage precursor cells in the mouse cerebral cortex: a confocal microscopy approach to demyelinating diseases.

  2010cites this paper
• Glioma Stem Cells in the Context of Oncogenesis

  2009cites this paper
• Fate determination of adult human glial progenitor cells.

  2009cites this paper
• Oligodendrocyte Development and the Onset of Myelination in the Human Fetal Brain

  2009cites this paper
• Interruption of β-Catenin Signaling Reduces Neurogenesis in Alzheimer's Disease

  2009cites this paper
• Postnatal Neurogenesis: From Neuroblast Migration to Integration into Mature Circuits

  2009cites this paper
• Effects of Methylprednisolone and Glatiramer Acetate on Nitric Oxide Formation of Cytokine-Stimulated Cells from the Rat Oligodendroglial Cell Line OLN-93

  2009cites this paper
• Postnatal neurogenesis: from neuroblast migration to neuronal integration.

  2009cites this paper
• Live imaging of remyelination after antibody-mediated demyelination in an ex-vivo model for immune mediated CNS damage.

  2009cites this paper
• High cytotoxic sensitivity of the oligodendrocyte precursor cells to HSP90 inhibitors in cell cultures.

  2009cites this paper
• miR-23 regulation of lamin B1 is crucial for oligodendrocyte development and myelination

  2009cites this paper
• The effect of CXCL1 on human fetal oligodendrocyte progenitor cells

  2008cites this paper
• Remyelination-Promoting Human IgMs: Developing a Therapeutic Reagent for Demyelinating Disease

  2008cites this paper
• Cerebral ependymoma in a patient with multiple sclerosis case report and critical review of the literature.

  2008cites this paper
• Distinctions between fetal and adult human platelet‐derived growth factor–responsive neural precursors

  2008influential citation
• Glial precursor cell transplantation therapy for neurotrauma and multiple sclerosis.

  2008cites this paper
• Myelin repair strategies: a cellular view

  2008cites this paper
• Statin treatment of adult human glial progenitors induces PPARγ‐mediated oligodendrocytic differentiation

  2008cites this paper
• Interruption of [beta]-Catenin Signaling Reduces Neurogenesis in Alzheimer's Disease Brains

  2008cites this paper
• In Vitro and In Vivo Pharmacological Models to Assess Demyelination and Remyelination

  2008influential citation
• Glial Progenitor-Like Phenotype in Low-Grade Glioma and Enhanced CD133-Expression and Neuronal Lineage Differentiation Potential in High-Grade Glioma

  2008cites this paper
• Glioma stem cells: evidence and limitation.

  2007cites this paper
• Multiple Sclerosis and Demyelinating Diseases

  2007cites this paper
• Multiple sclerosis and demyelination

  2007cites this paper
• Combination of Growth Factors Enhances Remyelination in a Cuprizone-induced Demyelination Mouse Model

  2007cites this paper
• Bone Morphogenetic Protein Signaling and Olig1/2 Interact to Regulate the Differentiation and Maturation of Adult Oligodendrocyte Precursor Cells

  2007cites this paper
• Role of endogenous neural stem cells in neurological disease and brain repair.

  2006cites this paper
• Endogenous Cell Repair of Chronic Demyelination

  2006cites this paper
• Neural Progenitor Cells of the Adult Human Brain

  2006cites this paper
• Co-expression of PDGF α receptor and NG2 by oligodendrocyte precursors in human CNS and multiple sclerosis lesions

  2006cites this paper
• Retroviral lineage analysis of fibroblast growth factor receptor signaling in FGF2 inhibition of oligodendrocyte progenitor differentiation

  2006cites this paper
• Endogenous stem and precursor cells for demyelinating diseases: an alternative for transplantation?

  2006cites this paper
• IGF-I and Brain Growth: Multifarious Effects on Developing Neural Cells and Mechanisms of Action

  2005cites this paper
• Human embryonic stem cell-derived oligodendrocyte progenitors for the treatment of spinal cord injury.

  2005cites this paper
• Anti-heat shock protein 90beta antibodies decrease pre-oligodendrocyte population in perinatal and adult cell cultures. Implications for remyelination in multiple sclerosis.

  2005cites this paper
• PDGF and FGF2 pathways regulate distinct oligodendrocyte lineage responses in experimental demyelination with spontaneous remyelination.

  2005cites this paper
• Mécanismes cellulaires et moléculaires de la croissance axonale

  2005cites this paper
• Neural stem cells and the origin of gliomas.

  2005cites this paper
• Progenitor Cells of the Adult Human Subcortical White Matter

  2004cites this paper
• Pathology and molecular genetics of oligodendroglial tumors

  2004cites this paper
• Oligodendroglioma and multiple sclerosis. A case report.

  2004cites this paper
• Distinctive properties of human adult brain-derived myelin progenitor cells.

  2004cites this paper
• Cell therapy in demyelinating diseases

  2004cites this paper
• Neural Cell Specification during Development

  2004cites this paper
• Metabotropic glutamate receptors are expressed in adult human glial progenitor cells.

  2004cites this paper