Modulation of cyclophosphamide-induced immunosuppression and intestinal flora in broiler by deep eutectic solvent extracted polysaccharides of Acanthopanax senticosus

Introduction The aim of this experiment was to investigate the modulation effect of Acanthopanax senticosus polysaccharide (ASPS-PD) extracted with deep eutectic solvent on cyclophosphamide-induced immunosuppression in broilers and its modulation of the gut microbiota of broilers. Methods The 108 one-day-old broilers were divided into six groups, including the control group, the Cyclophosphamide (CY) model group, the ASPS-PD control group, the ASPA-PD high and low dose groups and the Astragalus polysaccharide group. Body weight, feed intake, feed conversion ratio, and immune organ index of broilers at 7, 14, and 21 days were determined; IL-2, IFN-γ, and lgG1 levels were determined by enzyme-linked immunosorbent assay (ELISA); Broiler caeca feces were analyzed by amplification and 16S rRNA sequencing. Results The results showed that ASPS-PD can restore growth performance, increase immune organ index and improve serum cytokine levels of IL-2 and IFN-γ and immunoglobulin lgG1 levels in CY-treated broilers. The analysis of cecum flora showed that ASPS-PD can promote the proliferation of beneficial bacteria and reduce the number of harmful bacteria, regulating intestinal flora. Discussion Therefore, ASPA-PD may be a potential novel immunomodulator to ameliorate CY-induced immunosuppression and intestinal flora dysregulation in broiler.

• Publication year

  2024

• Venue

  Frontiers in Veterinary Science

• Publication date

  2024-05-27

• Fields of study

  Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.3389/fvets.2024.1415716PMID 38863455PMCID 11165361
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Eco-Friendly and Efficient Extraction of Polysaccharides from Acanthopanax senticosus by Ultrasound-Assisted Deep Eutectic Solvent

  2024cited by this paper
• Phytoimmunomodulators: A review of natural modulators for complex immune system

  2023cited by this paper
• Immunomodulatory Natural Polysaccharides: An Overview of the Mechanisms Involved

  2023cited by this paper
• Structure-effect relationship studies of polysaccharides based on receptor-active centres: an alternative view.

  2023cited by this paper
• The antioxidant activity of polysaccharides: A structure-function relationship overview.

  2023cited by this paper
• Research progress on the mechanisms underlying poultry immune regulation by plant polysaccharides

  2023cited by this paper
• Comparison of the Gut Microbial Communities of Domestic and Wild Mallards (Anas platyrhynchos) Based on High-Throughput Sequencing Technology

  2023cited by this paper
• Antioxidant Activity of Acanthopanax senticosus Flavonoids in H2O2-Induced RAW 264.7 Cells and DSS-Induced Colitis in Mice

  2022cited by this paper
• Effects of collagen peptides from Micropterus salmoides skin on oxidative damage induced by cyclophosphamide in mice

  2022cited by this paper
• The effects of dietary supplementation of Artemisia argyi polysaccharide on immune and antioxidative functions in broilers

  2022influential reference
• The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilers

  2022cited by this paper
• Microbiome-metabolome responses of Fuzhuan brick tea crude polysaccharides with immune-protective benefit in cyclophosphamide-induced immunosuppressive mice.

  2022cited by this paper
• Plant-Derived Polysaccharides Regulated Immune Status, Gut Health and Microbiota of Broilers: A Review

  2022cited by this paper
• Research Note: Effects of polysaccharide-enriched Acanthopanax senticosus extract on growth performance, immune function, antioxidation, and ileal microbial populations in broiler chickens

  2021cited by this paper
• Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

  2021cited by this paper
• Effects of Rhamnolipids on Growth Performance, Immune Function, and Cecal Microflora in Linnan Yellow Broilers Challenged with Lipopolysaccharides

  2021cited by this paper
• Transcriptome analysis reveals potential mechanisms of the effects of dietary Enteromorpha polysaccharides on bursa of Fabricius in broilers

  2021cited by this paper
• Sodium Alginate Modulates Immunity, Intestinal Mucosal Barrier Function, and Gut Microbiota in Cyclophosphamide-Induced Immunosuppressed BALB/c Mice.

  2021cited by this paper
• Algae-derived polysaccharides supplementation ameliorates heat stress-induced impairment of bursa of Fabricius via modulating NF-κB signaling pathway in broilers

  2021cited by this paper
• Protective effect of Polygonatum sibiricum polysaccharide on cyclophosphamide-induced immunosuppression in chickens.

  2021cited by this paper
• The Effect of Camellia oleifera Cake Polysaccharides on Growth Performance, Carcass Traits, Meat Quality, Blood Profile, and Caecum Microorganisms in Yellow Broilers

  2020cited by this paper
• The combined impact of xylo-oligosaccharides and gamma-irradiated Astragalus polysaccharides on growth performance and intestinal mucosal barrier function of broilers

  2020cited by this paper
• Immunomodulatory effect of Acanthopanax senticosus polysaccharide on immunosuppressed chickens

  2020cited by this paper
• [Effects of Short-term Application of Moutai-flavor Vinasse Biochar on Nitrogen Availability and Bacterial Community Structure Diversity in Yellow Soil of Guizhou Province].

  2020cited by this paper
• Mechanisms of Dysregulated Humoral and Cellular Immunity by SARS-CoV-2

  2020cited by this paper
• [Effects of Short-term Application of Moutai-flavor Vinasse Biochar on Nitrogen Availability and Bacterial Community Structure Diversity in Yellow Soil of Guizhou Province].

  2020cited by this paper
• Plant-Derived Nutraceuticals and Immune System Modulation: An Evidence-Based Overview

  2020cited by this paper
• The inhibitory effect of phosphorylated Codonopsis pilosula polysaccharide on autophagosomes formation contributes to the inhibition of duck hepatitis A virus replication

  2020cited by this paper
• New insights into the role of dietary marine-derived polysaccharides on productive performance, egg quality, antioxidant capacity, and jejunal morphology in late-phase laying hens

  2020cited by this paper
• Effect of Triptolide on Dextran Sodium Sulfate-Induced Ulcerative Colitis and Gut Microbiota in Mice

  2020cited by this paper
• Prevalence of H7N9 subtype avian influenza viruses in poultry in China, 2013-2018.

  2019cited by this paper
• Effects of glucose oxidase on growth performance, gut function, and cecal microbiota of broiler chickens

  2019cited by this paper
• DIABLO: an integrative approach for identifying key molecular drivers from multi-omics assays

  2019cited by this paper
• Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice.

  2019cited by this paper
• Effects of dietary inclusion of Lactobacillus and inulin on growth performance, gut microbiota, nutrient utilization, and immune parameters in broilers.

  2019cited by this paper
• Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice.

  2019cited by this paper
• Effects of dietary inulin supplementation on the composition and dynamics of cecal microbiota and growth-related parameters in broiler chickens

  2019cited by this paper
• Cultured Cordyceps sinensis polysaccharides attenuate cyclophosphamide-induced intestinal barrier injury in mice

  2019cited by this paper
• Serovar diversity of Salmonella among poultry

  2019cited by this paper
• Effects of dietary Lycium barbarum polysaccharides on growth performance, digestive enzyme activities, antioxidant status, and immunity of broiler chickens

  2019cited by this paper
• Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers

  2018cited by this paper
• Beneficial Effect of Intestinal Fermentation of Natural Polysaccharides

  2018cited by this paper
• Effects of Achyranthes bidentata Polysaccharides on Intestinal Morphology, Immune Response, and Gut Microbiome in Yellow Broiler Chickens Challenged with Escherichia coli K88

  2018cited by this paper
• Immunomodulatory effect of γ-irradiated Astragalus polysaccharides on immunosuppressed broilers.

  2018cited by this paper
• Dietary fucoidan improves metabolic syndrome in association with increased Akkermansia population in the gut microbiota of high-fat diet-fed mice

  2017cited by this paper
• Chemolithotrophic processes in the bacterial communities on the surface of mineral-enriched biochars

  2017cited by this paper
• Gut microbiota functions: metabolism of nutrients and other food components

  2017cited by this paper
• Gut microbiota: A player in aging and a target for anti-aging intervention

  2017cited by this paper
• The role of vaccination in risk mitigation and control of Newcastle disease in poultry.

  2017cited by this paper
• Systemic Immunomodulating Therapies for Stevens-Johnson Syndrome and Toxic Epidermal Necrolysis: A Systematic Review and Meta-analysis

  2017cited by this paper
• DADA2: High resolution sample inference from Illumina amplicon data

  2016cited by this paper
• Infectious bursal disease virus in poultry: current status and future prospects

  2016cited by this paper
• Bacteria within the Gastrointestinal Tract Microbiota Correlated with Improved Growth and Feed Conversion: Challenges Presented for the Identification of Performance Enhancing Probiotic Bacteria

  2016cited by this paper
• Effects of Clostridium butyricum on growth performance, antioxidation, and immune function of broilers.

  2015cited by this paper
• Comparison of fecal and cecal microbiotas reveals qualitative similarities but quantitative differences

  2015cited by this paper
• Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 20051

  2014cited by this paper
• Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2

  2014cited by this paper
• Untangling the Genetic Basis of Fibrolytic Specialization by Lachnospiraceae and Ruminococcaceae in Diverse Gut Communities

  2013cited by this paper
• Cordyceps militaris polysaccharides can improve the immune efficacy of Newcastle disease vaccine in chicken.

  2013cited by this paper
• Influence of penicillin on microbial diversity of the cecal microbiota in broiler chickens.

  2013cited by this paper
• Faecalibacterium prausnitzii and human intestinal health.

  2013cited by this paper
• Butyrate promotes the recovering of intestinal wound healing through its positive effect on the tight junctions.

  2012cited by this paper
• Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu.

  2011cited by this paper
• Metagenomic biomarker discovery and explanation

  2011cited by this paper
• Impact of digestive and oropharyngeal decontamination on the intestinal microbiota in ICU patients

  2010cited by this paper
• Sulfated modification can enhance the adjuvanticity of lentinan and improve the immune effect of ND vaccine.

  2009cited by this paper
• Synergy of Astragalus polysaccharides and probiotics (Lactobacillus and Bacillus cereus) on immunity and intestinal microbiota in chicks.

  2009cited by this paper
• Dietary supplementation with Acanthopanax senticosus extract enhances gut health in weanling piglets

  2009cited by this paper
• The gut microbiota shapes intestinal immune responses during health and disease

  2009cited by this paper
• Enrichment of bifidobacteria in the hen caeca by dietary inulin

  2008cited by this paper
• Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients

  2008cited by this paper
• Dietary Supplementation with Acanthopanax senticosus Extract Modulates Cellular and Humoral Immunity in Weaned Piglets

  2007cited by this paper
• Microbial ecology: Human gut microbes associated with obesity

  2006cited by this paper
• Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus.

  2003cited by this paper
• 16S rRNA-Based Analysis of Microbiota from the Cecum of Broiler Chickens

  2002cited by this paper
• Immunohistochemical investigations of lymphocytes in the lymphoid organs of cyclophosphamide treated chickens.

  1993cited by this paper
• Roles of dosage, pharmacokinetics, and cellular sensitivity to damage in the selective toxicity of cyclophosphamide towards B and T cells in development.

  1991cited by this paper
• The role of the thymus for maturation of transferred bursa cells into immunocompetent B cells in chickens treated with cyclophosphamide.

  1978cited by this paper
• Ontogeny of bursal function in chicken. I. Embryonic stem cell for humoral immunity.

  1972cited by this paper

• Polysaccharides from Acanthopanax senticosus (Rupr. et Maxim.) Harms: Molecular Messengers Linking the Traditional Efficacy of Fortifying the Spleen, Replenishing Qi, and Calming the Spirit to Gut-Immune Axis Regulation

  2026cites this paper
• Extraction, structural characteristics, bioactivities and application of polysaccharides from Acanthopanax senticosus (Rupr. Maxim.) harms: A review.

  2025cites this paper
• Selenium Broussonetia papyrifera polysaccharide alleviated cyclophosphamide-induced immune suppression, growth inhibition, intestinal damage, and gut microbiota disorder in yellow-feather broilers.

  2025cites this paper
• Acanthopanax senticosus polysaccharide alleviates LPS-induced intestinal inflammation in piglets by gut microbiota and hyodeoxycholic acid regulation.

  2025cites this paper
• Terahertz metasurfaces covered Weyl semimetal nanofilms for nondestructive detection of antibiotics

  2025cites this paper
• The effects of degraded polysaccharides from Acanthopanax senticosus on growth, antioxidant and immune effects in broiler chicks based on intestinal flora

  2025cites this paper
• A Comprehensive Review on Deep Eutectic Solvents: Their Current Status and Potential for Extracting Active Compounds from Adaptogenic Plants

  2024cites this paper