DHXT1, a Virulence Factor of Dactylellina haptotyla, Regulates Pathogenicity by Participating in Trap Formation and Metabolite Synthesis

The capsule-associated protein 10 gene (CAP10) is indispensable due to its involvement in pod formation and virulence maintenance in Cryptococcus neoformans. The function of the CAP10 gene in nematode-predatory fungi remains unreported. As a typical nematode-trapping fungus, Dactylellina haptotyla efficiently captures nematodes using adhesive knobs, which has potential applications in the biological control of plant-parasitic nematodes. In this study, we investigated the function of DHXT1 (a CAP10 homologous protein) in D. haptotyla–nematode interactions based on the disruption and overexpression of DHXT1, phenotypic analysis and metabolomic analysis. As a result, it was shown that the disruption of the DHXT1 gene causes a marked decrease in the number of adhesive knobs, and on the contrary, the overexpression of the DHXT1 gene causes a substantial increase in the number of adhesive knobs. Interestingly, the variety of metabolites increased with the disruption of the DHXT1 and decreased with the overexpression of the DHXT1 gene. The results suggest that DHXT1 effects pathogenicity through its involvement in adhesive knobs’ formation and metabolite synthesis and serves as a key virulence factor in D. haptotyla.

• Publication year

  2024

• Venue

  International Journal of Molecular Sciences

• Publication date

  2024-07-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/ijms25137384PMID 39000488PMCID 11242603
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Counter-attack of biocontrol agents: Environmentally benign Approaches against Root-knot nematodes (Meloidogyne spp.) on Agricultural crops

  2023cited by this paper
• Key processes required for the different stages of fungal carnivory by a nematode-trapping fungus

  2023cited by this paper
• Secondary Metabolites from the Nematode-Trapping Fungus Dactylellina haptotyla YMF1.03409

  2023cited by this paper
• 2-Furoic acid associated with the infection of nematodes by Dactylellina haptotyla and its biocontrol potential on plant root-knot nematodes

  2023influential reference
• Fatal attraction of Caenorhabditis elegans to predatory fungi through 6-methyl-salicylic acid

  2021cited by this paper
• Biological control: a novel strategy for the control of the plant parasitic nematodes

  2021cited by this paper
• New reduced-risk agricultural nematicides - rationale and review

  2020cited by this paper
• Expansion of Adhesion Genes Drives Pathogenic Adaptation of Nematode-Trapping Fungi

  2020influential reference
• Fungal and bacterial nematicides in integrated nematode management strategies

  2018cited by this paper
• Nematophagous fungus Arthrobotrys oligospora mimics olfactory cues of sex and food to lure its nematode prey

  2017cited by this paper
• Nematode-Trapping Fungi

  2017cited by this paper
• Potent Nematicidal Activity and New Hybrid Metabolite Production by Disruption of a Cytochrome P450 Gene Involved in the Biosynthesis of Morphological Regulatory Arthrosporols in Nematode-Trapping Fungus Arthrobotrys oligospora.

  2017cited by this paper
• Nematicidal Key Precursors for the Biosynthesis of Morphological Regulatory Arthrosporols in the Nematode-Trapping Fungus Arthrobotrys oligospora.

  2016cited by this paper
• A proposed adhesin AoMad1 helps nematode-trapping fungus Arthrobotrys oligospora recognizing host signals for life-style switching.

  2015cited by this paper
• Deletion of the CAP10 gene of Cryptococcus neoformans results in a pleiotropic phenotype with changes in expression of virulence factors.

  2014cited by this paper
• Genomics and Molecular Genetics of Plant-Nematode Interactions

  2011cited by this paper
• Production of Extracellular Polysaccharides by CAP Mutants of Cryptococcus neoformans

  2009cited by this paper
• Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling.

  2008cited by this paper
• A β-1,2-Xylosyltransferase from Cryptococcus neoformans Defines a New Family of Glycosyltransferases*

  2007cited by this paper
• Evolution of nematode-trapping cells of predatory fungi of the Orbiliaceae based on evidence from rRNA-encoding DNA and multiprotein sequences

  2007cited by this paper
• Microreview: Capsule-associated genes of Cryptococcus neoformans

  2006cited by this paper
• Microbial control of plant-parasitic nematodes: a five-party interaction

  2006cited by this paper
• Development and fate of electron-dense microbodies in trap cells of the nematophagous fungusArthrobotrys oligospora

  2005cited by this paper
• Do Organic Amendments Enhance the Nematode-Trapping Fungi Dactylellina haptotyla and Arthrobotrys oligospora?

  2004cited by this paper
• Correlations between most probable number and activity of nematode-trapping fungi.

  2003cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Isolation, Characterization, and Localization of a Capsule-Associated Gene, CAP10, of Cryptococcus neoformans

  1999cited by this paper
• Sequence analysis and regulation of a gene encoding a cuticle-degrading serine protease from the nematophagous fungus Arthrobotrys oligospora.

  1996cited by this paper
• Purification and characterization of an extracellular serine protease from the nematode-trapping fungus Arthrobotrys oligospora.

  1994cited by this paper
• Surface polymers of the nematode-trapping fungus Arthrobotrys oligospora.

  1991cited by this paper
• Proteases and Their Involvement in the Infection and Immobilization of Nematodes by the Nematophagous Fungus Arthrobotrys oligospora

  1991cited by this paper
• Development of fate of electron-dense microbodies in trap cells of the nematophagous fungus Arthrobotrys oligospora.

  1985cited by this paper
• Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 2 DD C T Method

  year unknowncited by this paper

• Calcium-regulated microbial mineralisation and corrosion of titanium: Mechanistic insights from Candida - Streptococcus biofilms and implications for Ca-containing implants

  2026cites this paper
• Infective Larvae of Haemonchus contortus (Nematoda: Trichostrongylidae) Are Captured and Destroyed by Nematode-Trapping Fungi Dactylellina spp. (Fungi: Orbiliales)

  2025cites this paper