Auditory hair cells and spiral ganglion neurons regenerate synapses with refined release properties in vitro

Significance Ribbon synapse loss between inner hair cells (IHCs) and type-I spiral ganglion neurons (SGNs) is responsible for sensorineural hearing loss (SNHL), often caused by exposure to traumatic sounds. Strategies employed so far to promote synapse regeneration do not provide evidence of functionality of the individual regenerated synapse, which would allow to ask whether the encoding properties of native mature IHC/type-I SGN synapses can be recapitulated by de novo synapses. This study adapts a preestablished coculture in vitro model that enables functional characterization of individual regenerated synapse properties by combining IHC stimulation and patch clamp recordings from newly connected SGN somata. This new approach provides a powerful tool for testing regenerative strategies with the aim of creating IHC-specific auditory synapses.

• Publication year

  2024

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  2024-07-26

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1073/pnas.2315599121PMID 39058581PMCID 11294990
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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