Systems-level reconstruction of kinase phosphosignaling networks regulating endothelial barrier integrity using temporal data

Phosphosignaling networks control cellular processes. We built kinase-mediated regulatory networks elicited by thrombin stimulation of brain endothelial cells using two computational strategies: Temporal Pathway Synthesizer (TPS), which uses phosphoproteomics data as input, and Temporally REsolved KInase Network Generation (TREKING), which uses kinase inhibitor screens. TPS and TREKING predicted overlapping barrier-regulatory kinases connected with unique network topology. Each strategy effectively describes regulatory signaling networks and is broadly applicable across biological systems.

• Publication year

  2024

• Venue

  bioRxiv

• Publication date

  2024-08-05

• Fields of study

  Biology, Medicine, Computer Science

• Identifiers
  DOI 10.1038/s41540-024-00468-9PMID 39548089PMCID 11326140
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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