12-O-Tetradecanoylphorbol-13-acetate-induced Dephosphorylation of Protein Kinase Cα Correlates with the Presence of a Membrane-associated Protein Phosphatase 2A Heterotrimer*

Protein kinase C signaling is desensitized through a combination of dephosphorylation and proteolysis in intact cells. The process of dephosphorylation is analyzed here, as well as its relationship to degradation. It is established for protein kinase Cα that dephosphorylation occurs in a membrane compartment following activation and temporally preceding significant degradation. The phosphatase responsible for the dephosphorylation appears to be a heterotrimeric type 2A phosphatase, which is shown to be in part constitutively membrane associated. Consistent with a role for this activity, okadaic acid is shown to inhibit the phorbol ester-induced dephosphorylation of protein kinase C that occurs in intact cells. Furthermore, phorbol ester-induced down-regulation of protein kinase Cα is shown not to be dependent on the rate of dephosphorylation, indicating that these desensitizing pathways may operate in parallel.

• Publication year

  1996

• Venue

  Journal of Biological Chemistry

• Publication date

  1996-12-20

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.271.51.32785PMID 8955114
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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