Ro ribonucleoproteins contribute to the resistance of Deinococcus radiodurans to ultraviolet irradiation.

The genome of the radiation-resistant eubacterium Deinococcus radiodurans contains an ortholog of an RNA-binding protein known as the Ro 60-kD autoantigen. This protein, which was previously identified only in higher eukaryotes, is normally bound to small RNAs known as Y RNAs. We show that the Ro protein ortholog Rsr contributes to the resistance of D. radiodurans to UV irradiation. Rsr binds several small RNAs, encoded upstream of rsr, that accumulate following UV irradiation. One of these RNAs resembles a Y RNA. These results suggest that Ro RNPs could similarly contribute to the recovery of higher cells following UV irradiation.

• Publication year

  2000

• Venue

  Genes & Development

• Publication date

  2000-04-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1101/GAD.14.7.777PMID 10766734PMCID PMC316496
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.

  1999cited by this paper
• The levels of the RoRNP-associated Y RNA are dependent upon the presence of ROP-1, the Caenorhabditis elegans Ro60 protein.

  1999cited by this paper
• Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1.

  1999influential reference
• Binding of the 60-kDa Ro autoantigen to Y RNAs: evidence for recognition in the major groove of a conserved helix.

  1998cited by this paper
• Against all odds: the survival strategies of Deinococcus radiodurans.

  1997influential reference
• A misfolded form of 5S rRNA is complexed with the Ro and La autoantigens.

  1996cited by this paper
• Antibody penetration into the cells of mice and men.

  1996cited by this paper
• Life with 6000 Genes

  1996cited by this paper
• Identification and characterization of uvrA, a DNA repair gene of Deinococcus radiodurans

  1996cited by this paper
• Autoantibodies in the diagnosis of systemicrheumatic diseases

  1995cited by this paper
• A perinucleolar compartment contains several RNA polymerase III transcripts as well as the polypyrimidine tract-binding protein, hnRNP I [published erratum appears in J Cell Biol 1995 Jul;130(2):497-500]

  1995cited by this paper
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  1995cited by this paper
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  1995cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
• A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors.

  1994cited by this paper
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  1994cited by this paper
• Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors.

  1993cited by this paper
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  1993cited by this paper
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  1993cited by this paper
• Promoter probe and shuttle plasmids for Deinococcus radiodurans.

  1992influential reference
• Molecular analysis of the 60-kDa human Ro ribonucleoprotein.

  1988cited by this paper
• Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA.

  1985cited by this paper
• The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.

  1984cited by this paper
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  1981cited by this paper
• Specific labeling of 3' termini of RNA with T4 RNA ligase.

  1980cited by this paper
• Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.

  1980cited by this paper
• Mapping adenines, guanines, and pyrimidines in RNA.

  1977cited by this paper
• Ultraviolet light effects on the bacterial cell.

  1968cited by this paper

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