Sequence‐Dependent Acylation of Peptide Lysine Residues by DNAzymes

Methods for modifying intact peptides are useful but can be unselective with regard to amino acid position and sequence context. In this work, we used in vitro selection to identify DNAzymes that acylate a Lys residue of a short peptide in sequence‐dependent fashion. The DNAzymes do not acylate Lys when placed at other residues in the peptide, and the acylation activity depends on the Lys sequence context. A high acylation yield is observed on the preparative nanomole scale. These findings are promising for further development of DNAzymes for broader application to top‐down Lys acylation of peptide and protein substrates.

• Publication year

  2024

• Venue

  ChemBioChem

• Publication date

  2024-09-06

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/cbic.202400578PMID 39239825PMCID PMC11543514
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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