Production of hydroxyl radical by decomposition of superoxide spin-trapped adducts.

The spin trapping of superoxide by nitrone spin traps, in particular 5,5-dimethyl-1-pyrroline-1-oxide (DMPO), was found to lead to the de novo production of hydroxyl radical and a nonradical species. Hydroxyl radical is then spin-trapped by DMPO, producing DMPO-OH. It was determined that a background level of approximately 3% DMPO-OH relative to DMPO-OOH is produced by this mechanism. Thus, the detection of hydroxyl radical by spin trapping with DMPO must be used with caution if the level of hydroxyl radical is less than 3% of the rate of superoxide generation. Several examples are cited in which investigators suggested that hydroxyl radical is produced when in fact the spin trapping of this free radical may have been an artifact of the system.

• Publication year

  1982

• Venue

  Molecular Pharmacology

• Publication date

  1982-03-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0026-895x(25)14604-xPMID 6285165
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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