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THE FORMATION OF AGGLUTININS WITHIN LYMPH NODES

P. D. McMaster,S. Hudack

Published 1934 in Journal of Experimental Medicine

ABSTRACT

Experiments were planned to determine whether or not lymph nodes form antibodies against antigens injected into the peripheral lymphatic capillaries. On 2 successive days killed cultures of B. paratyphosus-B were intradermally injected into the right ear of a large series of mice. The cervical lymph nodes on the right side became much enlarged while those on the left side appeared normal. At varying intervals up to 12 days after the last injection groups of 10 animals were etherized, bled for serum and the lymph nodes which drain the lymphatics of the ears excised. The right nodes were pooled in one group, the left in another. They were ground with sand, extracted with Ringer's solution and the extracts and serum titrated for agglutinins by 3 methods, the microscopic, the macroscopic and the centrifugation-macroscopic method of Gates. 1 On the second, third and fifth days after the last injection no agglutinins could be demonstrated in the node extracts or sera; but on the seventh day agglutinins were strongly positive in the extract from the nodes on the injected (right) side at a dilution of 1 in 120. They were questionably positive in the serum at a dilution of 1 in 30. The agglutinin titre was increased in both materials as procured on succeeding days, remaining slightly stronger in the node extract. At length, on the twelfth day, antibodies appeared in the extract from the nodes on the uninjected side.

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