Engineering Modular Biosensors to Confer Metabolite-Responsive Regulation of Transcription.

No abstract is available for this paper.

• Publication year

  2017

• Venue

  ACS Synthetic Biology

• Publication date

  2017-02-17

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1021/acssynbio.6b00184PMID 27744683
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Enzymatic protein switches built from paralogous input domains

  2016cited by this paper
• Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

  2016cited by this paper
• Promoter and Terminator Discovery and Engineering.

  2016cited by this paper
• Exploring lysine riboswitch for metabolic flux control and improvement of L-lysine synthesis in Corynebacterium glutamicum.

  2015cited by this paper
• A general strategy to construct small molecule biosensors in eukaryotes

  2015cited by this paper
• Design of allosterically regulated protein catalysts.

  2015cited by this paper
• Negative feedback regulation of fatty acid production based on a malonyl-CoA sensor-actuator.

  2015cited by this paper
• Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening.

  2015cited by this paper
• Dynamic metabolic engineering: New strategies for developing responsive cell factories

  2015cited by this paper
• Engineering metabolism through dynamic control.

  2015cited by this paper
• Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor.

  2015cited by this paper
• Synthetic biosensors for precise gene control and real-time monitoring of metabolites

  2015cited by this paper
• Synthetic fusion protein design and applications.

  2015cited by this paper
• Profiling metabolic states with genetically encoded fluorescent biosensors for NADH.

  2015cited by this paper
• Applications and advances of metabolite biosensors for metabolic engineering.

  2015cited by this paper
• Evolution-guided optimization of biosynthetic pathways

  2014cited by this paper
• The let-7/LIN-41 pathway regulates reprogramming to human induced pluripotent stem cells by controlling expression of prodifferentiation genes.

  2014cited by this paper
• Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes.

  2014cited by this paper
• Toehold switches: de-novo-designed regulators of gene expression.

  2014cited by this paper
• Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence

  2014cited by this paper
• Small RNA regulators in bacteria: powerful tools for metabolic engineering and synthetic biology

  2014cited by this paper
• Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras

  2014cited by this paper
• Genetically encoded fluorescent biosensors for live-cell visualization of protein phosphorylation.

  2014cited by this paper
• Recent advances in in vivo applications of intein-mediated protein splicing

  2014cited by this paper
• Characterization of 582 natural and synthetic terminators and quantification of their design constraints

  2013cited by this paper
• Engineering dynamic pathway regulation using stress-response promoters

  2013cited by this paper
• Computational design of ligand-binding proteins with high affinity and selectivity

  2013cited by this paper
• Screening for enhanced triacetic acid lactone production by recombinant Escherichia coli expressing a designed triacetic acid lactone reporter.

  2013cited by this paper
• An Engineered Calmodulin‐Based Allosteric Switch for Peptide Biosensing

  2013cited by this paper
• Transcription factor-based screens and synthetic selections for microbial small-molecule biosynthesis.

  2013cited by this paper
• Synthetic RNA devices to expedite the evolution of metabolite-producing microbes

  2013cited by this paper
• Promoter engineering: Recent advances in controlling transcription at the most fundamental level

  2013cited by this paper
• Rewiring cell signalling through chimaeric regulatory protein engineering

  2013cited by this paper
• A synthetic biology framework for programming eukaryotic transcription functions.

  2012cited by this paper
• Applications of genetically-encoded biosensors for the construction and control of biosynthetic pathways.

  2012cited by this paper
• A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level

  2012cited by this paper
• Engineering naturally occurring trans-acting non-coding RNAs to sense molecular signals

  2012cited by this paper
• A tunable zinc finger-based framework for Boolean logic computation in mammalian cells

  2012cited by this paper
• A transposase strategy for creating libraries of circularly permuted proteins

  2012cited by this paper
• Structure of an Engineered β-Lactamase Maltose Binding Protein Fusion Protein: Insights into Heterotropic Allosteric Regulation

  2012cited by this paper
• Iterative plug-and-play methodology for constructing and modifying synthetic gene networks

  2012cited by this paper
• Fluorescence-based biosensors.

  2012cited by this paper
• Novel insights from hybrid LacI/GalR proteins: family-wide functional attributes and biologically significant variation in transcription repression

  2012cited by this paper
• Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids

  2012cited by this paper
• Design and application of a mevalonate-responsive regulatory protein.

  2011cited by this paper
• Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

  2011cited by this paper
• Engineering Protein Switches: Sensors, Regulators, and Spare Parts for Biology and Biotechnology

  2011cited by this paper
• Biosensors and their applications in microbial metabolic engineering.

  2011cited by this paper
• TAL Effectors: Customizable Proteins for DNA Targeting

  2011cited by this paper
• A modular positive feedback-based gene amplifier

  2010cited by this paper
• High-throughput metabolic engineering: advances in small-molecule screening and selection.

  2010cited by this paper
• Automated Design of Synthetic Ribosome Binding Sites to Precisely Control Protein Expression

  2009cited by this paper
• Programming cells by multiplex genome engineering and accelerated evolution

  2009cited by this paper
• An engineered split M.HhaI-zinc finger fusion lacks the intended methyltransferase specificity.

  2008cited by this paper
• Linking the functions of unrelated proteins using a novel directed evolution domain insertion method

  2008cited by this paper
• Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli

  2008cited by this paper
• Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.

  2008cited by this paper
• Fluorescence resonance energy transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria

  2008cited by this paper
• Design and Application of Highly Responsive Fluorescence Resonance Energy Transfer Biosensors for Detection of Sugar in Living Saccharomyces cerevisiae Cells

  2007cited by this paper
• Monitoring of conformational change in maltose binding protein using split green fluorescent protein.

  2006cited by this paper
• Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly

  2006cited by this paper
• Development and use of fluorescent nanosensors for metabolite imaging in living cells.

  2005cited by this paper
• The TetR Family of Transcriptional Repressors

  2005cited by this paper
• Engineering allosteric protein switches by domain insertion.

  2005cited by this paper
• Creation of an allosteric enzyme by domain insertion.

  2004cited by this paper
• Cue-Signal-Response Analysis of TNF-Induced Apoptosis by Partial Least Squares Regression of Dynamic Multivariate Data

  2004cited by this paper
• Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.

  2004cited by this paper
• Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl‐homoserine lactones

  2004cited by this paper
• A molecular switch created by in vitro recombination of nonhomologous genes.

  2004cited by this paper
• Highly specific zinc finger proteins obtained by directed domain shuffling and cell-based selection

  2003cited by this paper
• Phenotypic alteration of eukaryotic cells using randomized libraries of artificial transcription factors

  2003cited by this paper
• Human zinc fingers as building blocks in the construction of artificial transcription factors

  2003cited by this paper
• Visualization of maltose uptake in living yeast cells by fluorescent nanosensors

  2002cited by this paper
• Converting a maltose receptor into a nascent binuclear copper oxygenase by computational design.

  2002cited by this paper
• Conversion of a maltose receptor into a zinc biosensor by computational design

  2001cited by this paper
• Design and selection of novel Cys2His2 zinc finger proteins.

  2001cited by this paper
• Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins

  2001cited by this paper
• Improving lycopene production in Escherichia coli by engineering metabolic control

  2000cited by this paper
• Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor.

  1997cited by this paper
• Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin

  1997cited by this paper
• The rational design of allosteric interactions in a monomeric protein and its applications to the construction of biosensors.

  1997cited by this paper
• Refined 1.8-A structure reveals the mode of binding of beta-cyclodextrin to the maltodextrin binding protein.

  1993cited by this paper
• The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis.

  1992cited by this paper
• Crystallographic evidence of a large ligand-induced hinge-twist motion between the two domains of the maltodextrin binding protein involved in active transport and chemotaxis.

  1992cited by this paper
• Atomic interactions in protein-carbohydrate complexes. Tryptophan residues in the periplasmic maltodextrin receptor for active transport and chemotaxis.

  1992cited by this paper
• Genetic approach to the role of tryptophan residues in the activities and fluorescence of a bacterial periplasmic maltose-binding protein.

  1990cited by this paper

• Expanding the bioanalytical application of β‐hydroxybutyrate binding proteins through characterization of their metabolite interactions and site‐directed mutagenesis

  2025cites this paper
• Computational design of Periplasmic binding protein biosensors guided by molecular dynamics

  2023cites this paper
• Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications.

  2023cites this paper
• Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in Corynebacterium glutamicum

  2023influential citation
• Engineering Ca2+-dependent DNA polymerase activity

  2023cites this paper
• A pathway independent multi-modular ordered control system based on thermosensors and CRISPRi improves bioproduction in Bacillus subtilis

  2022cites this paper
• Design and engineering of genetically encoded protein biosensors for small molecules.

  2022cites this paper
• Synthetic Protein Circuits and Devices Based on Reversible Protein-Protein Interactions: An Overview

  2021cites this paper
• In Vivo Biosensors for Directed Protein Evolution

  2021cites this paper
• Dynamic Control of Metabolism.

  2021cites this paper
• Multilayer Genetic Circuits for Dynamic Regulation of Metabolic Pathways.

  2021cites this paper
• Modular tuning engineering and versatile applications of genetically encoded biosensors

  2021cites this paper
• Synthetic Biology in Cyanobacteria and Applications for Biotechnology

  2021cites this paper
• De novo designed protein-interaction modules for in-cell applications.

  2020cites this paper
• Protein-Interaction Modules for In-Cell Applications.

  2020cites this paper
• Genetically-encoded biosensors for analyzing and controlling cellular process in yeast.

  2020cites this paper
• Advanced metabolic engineering strategies for the development of sustainable microbial processes

  2020cites this paper
• Reverse engineering of an aspirin-responsive transcriptional regulator in Escherichia coli.

  2019cites this paper
• Plant Synthetic Biology: Quantifying the “Known Unknowns” and Discovering the “Unknown Unknowns”1[OPEN]

  2019cites this paper
• Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion

  2019influential citation
• Engineering metabolite-responsive transcriptional factors to sense small molecules in eukaryotes: current state and perspectives

  2019cites this paper
• Custom-made transcriptional biosensors for metabolic engineering.

  2019cites this paper
• Screening and selection: Applications for vitamin biosynthesis in Escherichia coli

  2019cites this paper
• Biosensor-assisted high performing cell selection using an E. coli toxin/antitoxin system

  2019cites this paper
• Harnessing Marine Biocatalytic Reservoirs for Green Chemistry Applications through Metagenomic Technologies

  2018cites this paper
• Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion

  2018cites this paper
• Post-Translational Control over Metabolic Labeling of Newly Synthesized Proteins

  2018cites this paper
• In vivo biosensors: mechanisms, development, and applications

  2018cites this paper
• Reverse engineering of an aspirin-responsive regulator in bacteria

  2018cites this paper
• Challenges in the Application of Synthetic Biology Toward Synthesis of Commodity Products by Cyanobacteria via "Direct Conversion".

  2018cites this paper
• Boosting Secondary Metabolite Production and Discovery through the Engineering of Novel Microbial Biosensors

  2018cites this paper
• A Biosensor Strategy for E. coli Based on Ligand-Dependent Stabilization.

  2018cites this paper
• Developing a Genetically Encoded, Cross-Species Biosensor for Detecting Ammonium and Regulating Biosynthesis of Cyanophycin.

  2017cites this paper
• Dynamic pathway regulation: recent advances and methods of construction.

  2017cites this paper
• Towards Engineering a Programmable Universal Transcription Activation System

  2017cites this paper
• Sense and sensitivity in bioprocessing-detecting cellular metabolites with biosensors.

  2017cites this paper
• Programming Post-Translational Control over the Metabolic Labeling of Cellular Proteins with a Noncanonical Amino Acid.

  2017cites this paper
• Design and Selection of a Synthetic Feedback Loop for Optimizing Biofuel Tolerance.

  2017cites this paper
• New Intracellular Shikimic Acid Biosensor for Monitoring Shikimate Synthesis in Corynebacterium glutamicum.

  2017cites this paper
• Fundamental Design Principles for Transcription-Factor-Based Metabolite Biosensors.

  2017cites this paper