Ecological risks under combined pollution: Toxicological effects of clothianidin and microplastics on Penaeus vannamei.

In real-world conditions, multiple pollutants coexist, making it insufficient to study a single pollutant's effects. Clothianidin, a widely present neonicotinoid insecticide, poses significant ecological risks due to its unique mechanism of action. Similarly, microplastics, another prevalent pollutant, have notable ecological impacts. This study aims to assess the ecotoxicological effects of clothianidin and microplastics on P. vannamei over 28 days, both individually and in combination. The study found that combined exposure significantly inhibited the shrimp's weight gain rate and hepatosomatic index, and significant changes were observed in the shrimp's immune defense, signal transmission, and energy metabolism. Key findings include changes in levels of hemocyanin, respiratory burst, nitric oxide, and phenol oxidase, as well as disturbances in enzyme activities of Lactate dehydrogenase, NADPH-dependent isocitrate dehydrogenase, and Succinate dehydrogenase. Additionally, fluctuations in levels of acetylcholine, dopamine, and acetylcholinesterase confirmed disruptions in neural signaling. Transcriptomic analysis further revealed the profound impact of these pollutants on gene expression and metabolic processes in the hepatopancreas and nervous system. This comprehensive assessment highlights the potential impact on shrimp growth and emphasizes the ecological risks of clothianidin and microplastics, providing insights for future risk assessments and the identification of biomarkers.

• Publication year

  2025

• Venue

  Aquatic Toxicology

• Publication date

  2025-05-01

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.aquatox.2025.107417PMID 40412112
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dinotefuran exposure alters biochemical, metabolomic, gut microbiome, and growth responses in decapoda pacific white shrimp Penaeus vannamei.

  2024cited by this paper
• Adsorption of neonicotinoid insecticides by mulch film-derived microplastics and their combined toxicity.

  2024cited by this paper
• Transport of polyethylene and polypropylene microplastics under the action of agricultural chemicals: Role of pesticide adjuvants and neonicotinoid active ingredients.

  2024cited by this paper
• Interactions of traditional and biodegradable microplastics with neonicotinoid pesticides.

  2024cited by this paper
• The immunotoxicity mechanism of NH4Cl exposure to Litopenaeus vannamei based on the cerebral ganglion-eyestalk-haemocytes axis.

  2023cited by this paper
• Effects of neonicotinoid insecticides on transport of non-degradable agricultural film microplastics.

  2023cited by this paper
• Photoaged microplastics induce neurotoxicity associated with damage to serotonergic, glutamatergic, dopaminergic, and GABAergic neuronal systems in Caenorhabditis elegans.

  2023cited by this paper
• Selective HDAC6 inhibitor TubA offers neuroprotection after intracerebral hemorrhage via inhibiting neuronal apoptosis

  2023cited by this paper
• Polystyrene microplastics affect learning and memory in mice by inducing oxidative stress and decreasing the level of acetylcholine.

  2022cited by this paper
• Clothianidin interferes with recognition of a previous encounter in rusty crayfish (Faxonius rusticus) due to a chemosensory impairment.

  2022cited by this paper
• Environmental occurrence, toxicity concerns, and biodegradation of neonicotinoid insecticides.

  2022cited by this paper
• Size-dependent toxicological effects of polystyrene microplastics in the shrimp Litopenaeus vannamei using a histomorphology, microbiome, and metabolic approach authorship.

  2022cited by this paper
• Long-term effects of neonicotinoids on reproduction and offspring development in the copepod Acartia tonsa.

  2022cited by this paper
• Role of LDH in tumor glycolysis: Regulation of LDHA by small molecules for cancer therapeutics.

  2022cited by this paper
• Microbiome of Penaeus vannamei Larvae and Potential Biomarkers Associated With High and Low Survival in Shrimp Hatchery Tanks Affected by Acute Hepatopancreatic Necrosis Disease

  2022cited by this paper
• A Novel Ig Domain–Containing C-Type Lectin Triggers the Intestine–Hemocyte Axis to Regulate Antibacterial Immunity in Crab

  2022cited by this paper
• Combined toxic effects of thiamethoxam on intestinal flora, transcriptome and physiology of Pacific white shrimp Litopenaeus vannamei.

  2022cited by this paper
• Protein Diversity and Immune Specificity of Hemocyanin From Shrimp Litopenaeus vannamei

  2021cited by this paper
• Acute toxicity, accumulation and sublethal effects of four neonicotinoids on juvenile Black Tiger Shrimp (Penaeus monodon).

  2021cited by this paper
• Nitric oxide

  2021cited by this paper
• Clothianidin alters leukocyte profiles and elevates measures of oxidative stress in tadpoles of the amphibian, Rana pipiens.

  2021cited by this paper
• A comparative review of microplastics and nanoplastics: Toxicity hazards on digestive, reproductive and nervous system

  2021cited by this paper
• A Comprehensive Review: Sphingolipid Metabolism and Implications of Disruption in Sphingolipid Homeostasis

  2021cited by this paper
• Manifestations of oxidative stress and liver injury in clothianidin exposed Oncorhynchus mykiss.

  2021cited by this paper
• Chronic effects of clothianidin to non-target soil invertebrates: Ecological risk assessment using the species sensitivity distribution (SSD) approach.

  2021cited by this paper
• Effect of polyethylene microplastics on oxidative stress and histopathology damages in Litopenaeus vannamei.

  2021cited by this paper
• Physiological Effects of Neonicotinoid Insecticides on Non-Target Aquatic Animals—An Updated Review

  2021cited by this paper
• Impact of imidacloprid exposure on the biochemical responses, transcriptome, gut microbiota and growth performance of the Pacific white shrimp Litopenaeus vannamei.

  2021cited by this paper
• Toxicological effects of microplastics in Litopenaeus vannamei as indicated by an integrated microbiome, proteomic and metabolomic approach.

  2020cited by this paper
• Neonicotinoid Insecticides: Molecular Targets, Resistance, and Toxicity.

  2020cited by this paper
• A critical viewpoint on current issues, limitations, and future research needs on micro- and nanoplastic studies: From the detection to the toxicological assessment.

  2020cited by this paper
• Toxicological effects of nano- and micro-polystyrene plastics on red tilapia: Are larger plastic particles more harmless?

  2020cited by this paper
• New insights into anti-Müllerian hormone role in the hypothalamic–pituitary–gonadal axis and neuroendocrine development

  2020cited by this paper
• Acute and chronic effects of polystyrene microplastics on brine shrimp: First evidence highlighting the molecular mechanism through transcriptome analysis.

  2020cited by this paper
• Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos

  2020cited by this paper
• Fine-scale succession patterns and assembly mechanisms of bacterial community of Litopenaeus vannamei larvae across the developmental cycle

  2020cited by this paper
• Histidine Metabolism and Function.

  2020cited by this paper
• Environmental source, fate, and toxicity of microplastics.

  2020cited by this paper
• Impacts of microplastics on three different juvenile shrimps: Investigating the organism response distinction.

  2020cited by this paper
• Evidence of Marine Microplastics in Commercially Harvested Seafood

  2020cited by this paper
• Functional cytology of the hepatopancreas of decapod crustaceans

  2019cited by this paper
• Microplastics: Finding a consensus on the definition.

  2019cited by this paper
• Clothianidin seed-treatment has no detectable negative impact on honeybee colonies and their pathogens

  2019cited by this paper
• Exposure to acute ammonia stress influences survival, immune response and antioxidant status of pacific white shrimp (Litopenaeus vannamei) pretreated with diverse levels of inositol

  2019cited by this paper
• Neonicotinoid insecticides imidacloprid, guadipyr, and cycloxaprid induce acute oxidative stress in Daphnia magna.

  2018cited by this paper
• Succinate dehydrogenase (SDH)‐deficient neoplasia

  2018cited by this paper
• Can chronic exposure to imidacloprid, clothianidin, and thiamethoxam mixtures exert greater than additive toxicity in Chironomus dilutus?

  2018cited by this paper
• Biochemical Characterization and Structure-Based Mutational Analysis Provide Insight into the Binding and Mechanism of Action of Novel Aspartate Aminotransferase Inhibitors.

  2018cited by this paper
• Microplastic in marine organism: Environmental and toxicological effects.

  2018cited by this paper
• Thyroid Hormone Signaling Pathways: Time for a More Precise Nomenclature.

  2017cited by this paper
• Comparative chronic toxicity of imidacloprid, clothianidin, and thiamethoxam to Chironomus dilutus and estimation of toxic equivalency factors

  2017cited by this paper
• Effects of clothianidin on aquatic communities: Evaluating the impacts of lethal and sublethal exposure to neonicotinoids

  2017cited by this paper
• Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

  2016cited by this paper
• Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei.

  2015cited by this paper
• Neonicotinoid contamination of global surface waters and associated risk to aquatic invertebrates: a review.

  2015cited by this paper
• The concordance between RNA-seq and microarray data depends on chemical treatment and transcript abundance

  2014cited by this paper
• Biosynthesis and functions of sulfur modifications in tRNA

  2014cited by this paper
• Neonicotinoid-Contaminated Puddles of Water Represent a Risk of Intoxication for Honey Bees

  2014cited by this paper
• Eukaryotic ribosome biogenesis at a glance

  2013cited by this paper
• Policy: Classify plastic waste as hazardous

  2013cited by this paper
• Microplastics as contaminants in the marine environment: a review.

  2011cited by this paper
• Overview of the status and global strategy for neonicotinoids.

  2011cited by this paper
• The role of octopamine in locusts and other arthropods.

  2010cited by this paper
• Viral disease emergence in shrimp aquaculture: origins, impact and the effectiveness of health management strategies

  2009cited by this paper
• The Impact of Opioids on the Endocrine System

  2009cited by this paper
• Folate-mediated one-carbon metabolism.

  2008cited by this paper
• Minireview: Recent progress in hemocyanin research.

  2007cited by this paper
• The role of acetylcholine in learning and memory.

  2006cited by this paper
• Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis.

  2006cited by this paper
• A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress.

  2005cited by this paper
• Cell biological consequences of mitochondrial NADH: ubiquinone oxidoreductase deficiency.

  2004cited by this paper
• The prophenoloxidase‐activating system in invertebrates

  2004cited by this paper
• Decreased cardiac mitochondrial NADP+-isocitrate dehydrogenase activity and expression: a marker of oxidative stress in hypertrophy development.

  2004cited by this paper
• Tumor necrosis factor signaling

  2003cited by this paper
• G proteins and phototransduction.

  2002cited by this paper
• The role of nitric oxide in tissue destruction.

  2001cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific White Shrimp, Litopenaeus vannamei, and the Atlantic White Shrimp, L. setiferus.

  2001cited by this paper
• Release of digestive enzymes from the crustacean hepatopancreas: effect of vertebrate gastrointestinal hormones.

  1999cited by this paper
• Folate metabolism and requirements.

  1999cited by this paper
• Apoptosis: an overview.

  1997cited by this paper
• Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species.

  1994cited by this paper
• Neuroendocrine Axis and Behavioral Stress

  1994cited by this paper
• A noncholinergic action of acetylcholinesterase (AChE) in the brain: From neuronal secretion to the generation of movement

  1991cited by this paper
• The respiratory burst oxidase.

  1988cited by this paper
• Oxidative phosphorylation and photophosphorylation.

  1977cited by this paper
• Purine metabolism.

  1975cited by this paper
• Purification and characterization of pre-phenoloxidase from hemolymph of the silkworm Bombyx mori.

  1971cited by this paper
• A comparison of molluscan and arthropod hemocyanin—I. Circular dichroism and absorption spectra

  1971cited by this paper
• Protein digestion and absorption.

  1971cited by this paper
• Amino acid metabolism.

  1962cited by this paper
• The mechanism of pancreatic secretion

  1902cited by this paper

• Hydrodynamic modulation of microplastic bioaccumulation in edible fish: Integrating biomarker networks, machine learning, and food safety perspectives.

  2026cites this paper
• Transcriptomic insights into an ancient and endangered species: Adverse effects of nanopolystyrene on the behavior and energy metabolism of Tachypleus tridentatus.

  2025cites this paper