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Characterization of the JAZ family in Osmanthus fragrans and the role of OfLJAZ2 in flavonoid synthesis mediated regulation of salt and drought stress tolerance.

Wenle Li,Shumin Yu,Kaimei Zhang,Shunran Zhang,Yan Xiang,Y. Lan

Published 2025 in Plant physiology and biochemistry : PPB

ABSTRACT

As an important ornamental plant, the application of Osmanthus fragrans in landscape gardening is limited by abiotic stresses such as drought and salinity. JAZs, as key negative regulators in the jasmonic acid signaling pathway, play crucial roles in regulating stress responses in plants. However, the JAZ family in O. fragrans remains insufficiently characterized. Here, 75 OfJAZs were identified in four O. fragrans cultivars ('Liuyejingui', 'Yulianyinsi', 'Rixianggui', and 'Zhuangyuanhong'), and their phylogenetic relationships, gene structures, conserved motifs and cis-acting elements characteristics were systematically analyzed. Evolutionary analysis revealed that the OfJAZs primarily expanded through whole-genome duplication events. Further functional studies demonstrated that overexpression of OfLJAZ2 significantly inhibited flavonoid biosynthesis and reduced salt and drought stress tolerance in transgenic plants. Dual-LUC and EMSA assays revealed that OfLJAZ2 interacts with OfLMYB21 to form a transcriptional complex, which suppresses OfLMYB21 transcriptional activation of the flavonol synthase OfFLS, thereby reducing flavonoid accumulation. Conversely, overexpression of OfLMYB21 increased salt and drought stress tolerance and promoted flavonoid biosynthesis in transgenic plants. This study provided the first genome-wide identification of OfJAZs in different O. fragrans cultivars and preliminarily revealed the molecular mechanism by the key JAZ protein OfLJAZ2 regulated flavonoid metabolism to participate in salt and drought stress tolerance, which provided a theoretical basis for molecular breeding of stress resistance O. fragrans cultivars.

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