Transcriptome sequencing flower petals reveals insights into regulation of flavonoid biosynthesis in Osmanthus fragrans

Osmanthus fragrans Lour., one of the top 10 most popular flowers in China, is known for both its beauty and fragrance. It is rich in flavonoids, a class of secondary metabolites with significant neuroprotective, free-radical scavenging, and anti-oxidant activity. To understand the mechanisms regulating flavonoid biosynthesis, we conducted transcriptome sequencing O. fragrans flowers to analyze gene expressions during the full flowering stage. The RNA was isolated separately from petals of cvs. Yingui and Dangui, which were treated or not with jasmonic acid, salicylic acid, or abscisic acid. A total of 142 029 unigenes were denovo assembled, and 50 918 unigenes were annotated. The differentially expressed genes were identified, annotated, and classified. The results of transcriptome sequencing and real-time PCR revealed higher expressions of phenylalanine ammonia-lyase (PAL), PAL1, chalcone synthase (CHS), flavanone-3-hydroxylase (F3H), flavonol synthase (FLS), and lower expressions of dihydroflavonol-4-reductase (DFR), anthocyanidin synthase (ANS) in 'Yingui' than in 'Dangui'. Such an expression pattern facilitated the higher accumulation of flavonoids in 'Yingui'. Several genes of the flavonoid biosynthesis pathway were upregulated by jasmonic acid and salicylic acid in both the cultivars leading to flavonoid accumulation in their petals. In the v-myb avian myeloblastosis viral oncogene homolog 1(MYB1)-overexpressing petals, the expressions of PAL, PAL1, CHI, and FLS increased. The results suggest that MYB1 may participate in the flavonoid biosynthesis pathway and regulate the expression of some upstream genes in O. fragrans.

• Publication year

  2019

• Venue

  Biologia Plantarum

• Publication date

  2019-12-18

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.32615/bp.2019.146
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents, β-ionone and linalool

  2019cited by this paper
• Genome-wide sequential, evolutionary, organizational and expression analyses of phenylpropanoid biosynthesis associated MYB domain transcription factors in Arabidopsis

  2018cited by this paper
• The First Genetic Map in Sweet Osmanthus (Osmanthus fragrans Lour.) Using Specific Locus Amplified Fragment Sequencing

  2017cited by this paper
• Characterization of OfWRKY3, a transcription factor that positively regulates the carotenoid cleavage dioxygenase gene OfCCD4 in Osmanthus fragrans

  2016cited by this paper
• Trinity : reconstructing a full-length transcriptome without a genome from RNA-Seq data

  2016influential reference
• cDNA-AFLP analysis on 2 Osmanthus fragrans cultivars with different flower color and molecular characteristics of OfMYB1 gene

  2015cited by this paper
• Mitogen-activated protein kinase 6 controls root growth in Arabidopsis by modulating Ca2+ -based Na+ flux in root cell under salt stress.

  2014cited by this paper
• Differential expression of carotenoid-related genes determines diversified carotenoid coloration in flower petal of Osmanthus fragrans

  2014cited by this paper
• Salicylic acid treatment enhances expression of chalcone isomerase gene and accumulation of corresponding flavonoids during fruit maturation of Lycium chinense

  2014cited by this paper
• An apple MYB transcription factor, MdMYB3, is involved in regulation of anthocyanin biosynthesis and flower development

  2013cited by this paper
• Elicitation of flavonoids by Salicylic acid and Penicillium expansum in Andrographis paniculata (Burm. f.) Nees. cell culture

  2013cited by this paper
• cDNA cloning of the phytoene synthase (PSY) and expression analysis of PSY and carotenoid cleavage dioxygenase genes in Osmanthus fragrans

  2013cited by this paper
• Pfam: the protein families database

  2013influential reference
• The Jasmonate-ZIM-Domain Proteins Interact with the WD-Repeat/bHLH/MYB Complexes to Regulate Jasmonate-Mediated Anthocyanin Accumulation and Trichome Initiation in Arabidopsis thaliana[C][W]

  2011cited by this paper
• Trinity: reconstructing a full-length transcriptome without a genome from RNA-Seq data

  2011influential reference
• Characterization of the sesame (Sesamum indicum L.) global transcriptome using Illumina paired-end sequencing and development of EST-SSR markers

  2011cited by this paper
• Gene Expression and Metabolite Profiling of Developing Highbush Blueberry Fruit Indicates Transcriptional Regulation of Flavonoid Metabolism and Activation of Abscisic Acid Metabolism1[W][OA]

  2011cited by this paper
• Fungal endophytes-induced abscisic acid is required for flavonoid accumulation in suspension cells of Ginkgo biloba

  2010cited by this paper
• Subgroup 4 R2R3-MYBs in conifer trees: gene family expansion and contribution to the isoprenoid- and flavonoid-oriented responses

  2010cited by this paper
• RNA-Seq: a revolutionary tool for transcriptomics

  2009cited by this paper
• The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries1[W][OA]

  2009cited by this paper
• Seeing is believing: engineering anthocyanin and carotenoid biosynthetic pathways.

  2008cited by this paper
• High-throughput gene and SNP discovery in Eucalyptus grandis, an uncharacterized genome

  2008cited by this paper
• Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings.

  2008cited by this paper
• Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids.

  2008cited by this paper
• Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10

  2007cited by this paper
• Accuracy and quality of massively parallel DNA pyrosequencing

  2007cited by this paper
• Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling

  2007cited by this paper
• Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes

  2007cited by this paper
• Redirection of anthocyanin synthesis in Osteospermum hybrida by a two-enzyme manipulation strategy.

  2007cited by this paper
• Isolation of cDNAs for R2R3-MYB, bHLH and WDR transcriptional regulators and identification of c and ca mutations conferring white flowers in the Japanese morning glory.

  2006cited by this paper
• RNAi suppression of the anthocyanidin synthase gene in Torenia hybrida yields white flowers with higher frequency and better stability than antisense and sense suppression

  2006cited by this paper
• PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin Pathway[W]

  2006influential reference
• A Small Family of MYB-Regulatory Genes Controls Floral Pigmentation Intensity and Patterning in the Genus Antirrhinum[W]

  2006cited by this paper
• Light-Induced Expression of a MYB Gene Regulates Anthocyanin Biosynthesis in Red Apples1

  2006cited by this paper
• MYB-bHLH-WD40 protein complex and the evolution of cellular diversity.

  2005cited by this paper
• Genome sequencing in microfabricated high-density picolitre reactors

  2005cited by this paper
• Transcriptional control of flavonoid biosynthesis: a complex network of conserved regulators involved in multiple aspects of differentiation in Arabidopsis.

  2005cited by this paper
• The Arabidopsis Transcription Factor MYB12 Is a Flavonol-Specific Regulator of Phenylpropanoid Biosynthesis1

  2005cited by this paper
• Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene

  2005cited by this paper
• Flavonoids: a colorful model for the regulation and evolution of biochemical pathways.

  2005cited by this paper
• Flower color modification of Petunia hybrida commercial varieties by metabolic engineering

  2004cited by this paper
• Different Mechanisms Participate in the R-dependent Activity of the R2R3 MYB Transcription Factor C1*

  2004cited by this paper
• The KEGG resource for deciphering the genome

  2004cited by this paper
• Gene Ontology Consortium: The Gene Ontology (GO) database and informatics resource

  2004cited by this paper
• TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana.

  2004cited by this paper
• The COG database: an updated version includes eukaryotes

  2003cited by this paper
• Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.012963.

  2003cited by this paper
• Modification of flower color and fragrance by antisense suppression of the flavanone 3-hydroxylase gene

  2002cited by this paper
• Orthology, paralogy and proposed classification for paralog subtypes.

  2002cited by this paper
• Molecular analysis of herbivore-induced condensed tannin synthesis: cloning and expression of dihydroflavonol reductase from trembling aspen (Populus tremuloides).

  2002cited by this paper
• High-Flavonol Tomatoes Resulting from the Heterologous Expression of the Maize Transcription Factor Genes LC and C1 Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.004218.

  2002cited by this paper
• The TRANSPARENT TESTA12 Gene of Arabidopsis Encodes a Multidrug Secondary Transporter-like Protein Required for Flavonoid Sequestration in Vacuoles of the Seed Coat Endothelium

  2001cited by this paper
• Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology.

  2001cited by this paper
• The Arabidopsis TT2 Gene Encodes an R2R3 MYB Domain Protein That Acts as a Key Determinant for Proanthocyanidin Accumulation in Developing Seed

  2001cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• anthocyanin1 of Petunia Encodes a Basic Helix-Loop-Helix Protein That Directly Activates Transcription of Structural Anthocyanin Genes

  2000cited by this paper
• Molecular Analysis of the anthocyanin2 Gene of Petunia and Its Role in the Evolution of Flower Color

  1999influential reference
• The SWISS-PROT protein sequence data bank and its supplement TrEMBL in 1998

  1998influential reference
• Basic local alignment search tool.

  1990cited by this paper

• The AP2/ERF transcription factor OfERF2 promotes flavonoid biosynthesis in Osmanthus fragrans

  2025cites this paper
• Characterization of the JAZ family in Osmanthus fragrans and the role of OfLJAZ2 in flavonoid synthesis mediated regulation of salt and drought stress tolerance.

  2025cites this paper
• Integrated transcriptome and metabolome provide insight into flavonoid variation in goji berries (Lycium barbarum L.) from different areas in China.

  2023cites this paper
• Edible Osmanthus fragrans flowers: aroma and functional components, beneficial functions, and applications

  2023influential citation
• Coloration differences in three Meconopsis species: M. punicea, M. integrifolia and M. wilsonnii

  2022cites this paper
• Integrative Analyses of Metabolomes and Transcriptomes Provide Insights into Flavonoid Variation in Grape Berries.

  2021cites this paper