Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants

Salinity limits crop productivity, in part by decreasing shoot concentrations of the growth-promoting and senescence-delaying hormones cytokinins. Since constitutive cytokinin overproduction may have pleiotropic effects on plant development, two approaches assessed whether specific root-localized transgenic IPT (a key enzyme for cytokinin biosynthesis) gene expression could substantially improve tomato plant growth and yield under salinity: transient root IPT induction (HSP70::IPT) and grafting wild-type (WT) shoots onto a constitutive IPT-expressing rootstock (WT/35S::IPT). Transient root IPT induction increased root, xylem sap, and leaf bioactive cytokinin concentrations 2- to 3-fold without shoot IPT gene expression. Although IPT induction reduced root biomass (by 15%) in control (non-salinized) plants, in salinized plants (100 mM NaCl for 22 d), increased cytokinin concentrations delayed stomatal closure and leaf senescence and almost doubled shoot growth (compared with WT plants), with concomitant increases in the essential nutrient K+ (20%) and decreases in the toxic ion Na+ (by 30%) and abscisic acid (by 20–40%) concentrations in transpiring mature leaves. Similarly, WT/35S::IPT plants (scion/rootstock) grown with 75 mM NaCl for 90 d had higher fruit trans-zeatin concentrations (1.5- to 2-fold) and yielded 30% more than WT/non-transformed plants. Enhancing root cytokinin synthesis modified both shoot hormonal and ionic status, thus ameliorating salinity-induced decreases in growth and yield.

• Publication year

  2010

• Venue

  Journal of Experimental Botany

• Publication date

  2010-10-19

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1093/jxb/erq266PMID 20959628PMCID 2993914
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2009influential reference
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  2009influential reference
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  2008influential reference
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  2008influential reference
• Cytokinins are central regulators of cambial activity

  2008influential reference
• Mechanisms of salinity tolerance.

  2008influential reference
• Cytokinin profiling in plant tissues using ultra-performance liquid chromatography-electrospray tandem mass spectrometry.

  2008cited by this paper
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  2008influential reference
• Phytoremediation of Sodic and Saline‐Sodic Soils

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• Senescence processes in plants

  2007cited by this paper
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  2007influential reference
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  2007cited by this paper
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  2007cited by this paper
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• A hydraulic signal in root-to-shoot signalling of water shortage.

  2007cited by this paper
• Over-expression of a vacuolar Na+/H+ antiporter gene improves salt tolerance in an upland rice

  2007influential reference
• Effects of cytokinin production under two SAG promoters on senescence and development of tomato plants.

  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2005influential reference
• Expression of the ipt Gene with the AGPase S1 Promoter in Tomato Results in Unbranched Roots and Delayed Leaf Senescence

  2005cited by this paper
• Root-To-Shoot Signalling: Assessing The Roles of ‘Up’ In the Up and Down World of Long-Distance Signalling In Planta

  2005cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2004influential reference
• AtIPT3 is a key determinant of nitrate-dependent cytokinin biosynthesis in Arabidopsis.

  2004cited by this paper
• Increase of endogenous zeatin riboside by introduction of the ipt gene in wild type and the lateral suppressor mutant of tomato

  2004cited by this paper
• Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and nitrate.

  2004cited by this paper
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  2003cited by this paper
• Quantitative analysis of cytokinins in plants by liquid chromatography–single-quadrupole mass spectrometry

  2003cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2002influential reference
• Stomatal control in tomato with ABA-deficient roots: response of grafted plants to soil drying.

  2002cited by this paper
• New Insights into the Functions of Cytokinins in Plant Development

  2002cited by this paper
• Tribute to Folke Skoog: Recent Advances in our Understanding of Cytokinin Biology

  2002cited by this paper
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  2001cited by this paper
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  2001influential reference
• Regulation of plant growth by cytokinin

  2001cited by this paper
• Composition of xylem and phloem exudates in relation to the salt-tolerance of domestic and wild tomato species.

  2000cited by this paper
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  2000influential reference
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  2000cited by this paper
• Photosynthesis of Transgenic Pssu-ipt Tobacco

  1999influential reference
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  1998cited by this paper
• Relative importance of osmotic‐stress and ion‐specific effects on ABA‐mediated inhibition of leaf expansion growth in Phaseolus vulgaris

  1998cited by this paper
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  1998influential reference
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  1997influential reference
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  1996cited by this paper
• A system for tissue-specific copper-controllable gene expression in transgenic plants: Nodule-specific antisense of asparate aminotransferase-P2

  1996cited by this paper
• Inhibition of Leaf Senescence by Autoregulated Production of Cytokinin

  1995influential reference
• Cytokinins: Chemistry, Activity, and Function

  1994cited by this paper
• Are Plant Hormones Involved in Root to Shoot Communication

  1993cited by this paper
• Cytokinin effects on root growth and possible interactions with ethylene and indole‐3‐acetic acid

  1992cited by this paper
• Delayed Leaf Senescence in Tobacco Plants Transformed with tmr, a Gene for Cytokinin Production in Agrobacterium.

  1991cited by this paper
• Root Signals and the Regulation of Growth and Development of Plants in Drying Soil

  1991cited by this paper
• Cytokinins in the Phloem Sap of White Lupin (Lupinus albus L.).

  1990cited by this paper
• Cytokinin-to-Auxin Ratios and Morphology of Shoots and Tissues Transformed by a Chimeric Isopentenyl Transferase Gene.

  1989cited by this paper
• Estimation of osmotic parameters accompanying zeatin-induced growth of detached cucumber cotyledons.

  1982cited by this paper
• Effects of Salt Stress on Abscisic Acid and Cytokinin Levels in Tomato

  1981cited by this paper
• Cytokinin Activity in Water-stressed Shoots.

  1971cited by this paper
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  year unknowncited by this paper

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