A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

No abstract is available for this paper.

• Publication year

  1977

• Venue

  Analytical Biochemistry

• Publication date

  1977-05-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/0003-2697(77)90428-6PMID 68686
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Sensitive fluorometric assay for proteins: use of fluorescamine and membrane filters.

  1976cited by this paper
• Assay of proteins in the presence of interfering materials.

  1976cited by this paper
• The use of Coomassie Brilliant Blue G250 perchloric acid solution for staining in electrophoresis and isoelectric focusing on polyacrylamide gels.

  1975cited by this paper
• Microassay of proteins on membrane filter in the nanogram range using cycloheptaamylase-dansyl chloride complex.

  1975cited by this paper
• A rapid, sensitive, and specific method for the determination of protein in dilute solution.

  1973cited by this paper
• Microdetermination of protein not affected by the presence of various buffers, sucrose, ATP, and eluates from polysaccharide derivatives.

  1973cited by this paper
• Fluorometric assay of proteins in the nanogram range.

  1973cited by this paper
• The basic trypsin inhibitor of bovine pancreas. VI. Sequence studies and disulphide linkages.

  1966cited by this paper
• Two new staining procedures for quantitative estimation of proteins on electrophoretic strips.

  1963cited by this paper
• Amino acid metabolism in mammalian cell cultures.

  1959cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Polysaccharides from Pleurotus eryngii: sequential extraction and structural characterization

  2026cites this paper
• Citrus peel polysaccharide attenuates high-sugar/high-fat diet-induced hyperlipidemic pancreatitis via the gut-pancreas axis.

  2026cites this paper
• Synergistic effects of calcium lignosulfonate and microorganisms on saline-alkali leachate remediation: Enhancing plant growth and shaping rhizosphere microbial communities

  2026cites this paper
• Kaolin particle film reduces drought stress in grapevines and regulates photosynthesis and antioxidant capacity.

  2026cites this paper
• Comprehensive physiological and transcriptomic analysis revealed the tolerance mechanism of heavy metal cadmium in Quercus dentata.

  2026cites this paper
• Autoclaved Peanuts Exhibit Reduced Immunoglobulin E Binding and Improved Oral Tolerability.

  2026cites this paper
• Dual-configuration arabinogalactan-II synergizes with rhamnogalacturonan-I in Lycium barbarum pectin to construct an intestinal anti-inflammatory glycan scaffold

  2026cites this paper
• Pectic polysaccharides from tea residues: Structural characterization and molecular evolution throughout in vitro fecal fermentation.

  2026cites this paper
• An enzyme-based enhanced extraction and structural characterization of pectic polysaccharides from Panax notoginseng marc

  2026cites this paper
• Rhizophagus irregularis inoculation reshapes root exudates, metabolomes and rhizosphere microbiomes to enhance perennial ryegrass adaptation under heat and cold stress

  2026cites this paper
• Lomitapide reduces viability and clonogenicity in hepatocellular carcinoma cells but enhances xenograft growth: The importance of the tumor microenvironment.

  2026cites this paper
• Solvatochromic Study of Brilliant Blue G: Estimation of the Electric Dipole Moment in the Excited State

  2026cites this paper
• Effects of nitrogen fertilizer on protein accumulation in basal-middle and apical kernels of different low nitrogen tolerant maize hybrids

  2025cites this paper
• Optimisation of nutritional value, fibre degradation, and microbial diversity in hybrid Pennisetum silage via white-rot fungal fermentation for improved bioresource utilisation.

  2025cites this paper
• Boosting volatile fatty acids (VFAs) production during anaerobic digestion of rich lignocellulose pulp mill excess sludge by inoculating rumen fluid and the study on the microbial community structures and functions.

  2025cites this paper
• Decrease in egg quality due to abnormal ovulation in Atlantic salmon (Salmo salar)

  2025cites this paper
• Effects of prolonged fasting on substrate metabolism in female Japanese quails.

  2025cites this paper
• Preliminary Investigation Towards a Safety Tool for Swine Brucellosis Diagnosis by a Proteomic Approach Within the One-Health Framework

  2025cites this paper
• Synergistic effect of small molecule oligopeptides and rhamnolipids on enhancing cold stress tolerance in tea plants: insights from physiological and transcriptome analyses

  2025cites this paper
• Selective Sensing of Human Serum Albumin by a D···π···A-Based Self-Assembled Nanoprobe via a Disassembly Mechanism: Quantification and Live Cell Imaging Applications.

  2025cites this paper
• Asparagopsis taxiformis mitigates ruminant methane emissions via microbial modulation and inhibition of methyl-coenzyme M reductase

  2025cites this paper
• Effective role and mechanism of scrap iron filings in controlling hydrogen sulfide production in septic tanks.

  2025cites this paper
• High-quality carrots in relation to the superior phloem parenchyma cells and proportion of xylem vessel in coordinated water-fertilizer management

  2025cites this paper
• Transcriptomic and metabonomic profiling unveils the mechanism of Tartary buckwheat and kiwi co-fermentation products in hyperlipidemia treatment

  2025cites this paper
• Enhancing sweet sorghum emergence and stress resilience in saline-alkaline soils through ABA seed priming: insights into hormonal and metabolic reprogramming

  2025cites this paper
• One-Pot Microwave-Assisted Synthesis of Fluorescent Carbon Dots from Tomato Industry Residues with Antioxidant and Antibacterial Activities

  2025cites this paper
• Effects of seed priming with La2O3 nanoparticles on seed vigor of alfalfa (Medicago sativa) under salt stress

  2025cites this paper
• Agronomic and Quality Traits of 30 Eggplant Germplasm Resources from China

  2025cites this paper
• Assessment of the growth and quality of pepper seedlings under the combinations of daily light integral and nitrogen concentration

  2025cites this paper
• Enhancement of total ammonia and nitrite removal, growth, and vibrio tolerance in whiteleg shrimp (Penaeus vannamei) by Paracoccus marcusii ND07 administration in rearing water

  2025cites this paper
• Comparative physiological and co-expression network analysis reveals potential hub genes and adaptive mechanisms responsive to NaCl stress in peanut (Arachis hypogaea L.)

  2025cites this paper
• Enhanced moving bed biofilm reactor (MBBR) and an upflow anaerobic sludge blanket (UASB) reactor for landfill leachate treatment: Two-stage partial nitrification-anammox process

  2025cites this paper
• Allelopathic effects and mechanism of kaempferol on controlling Microcystis aeruginosa blooms.

  2025cites this paper
• Metabolic Activity of Invasive Apple Snails Negatively Affects the Survival of Native Benthic Snail in Mangrove

  2025cites this paper
• Innovative dual-contrast nanocoating for central venous catheters: prolonged infection resistance and enhanced imaging.

  2025cites this paper
• Leaf photosynthesis and antioxidant capacity are rapidly restored by Mn3O4 nanoparticles in rice plants under Cd stress without affecting plant Cd concentration.

  2025cites this paper
• Salicylic Acid Alleviates Cold Stress in Rice via Regulating Nutrient Absorption, Osmotic Material Content, Antioxidation System, and Expression of Cold Tolerance Genes

  2025cites this paper
• Digestion Characteristics and Probiotic Activity of An Tea Polysaccharides: Promoting Lactobacillus and Bifidobacterium In Vitro and In Vivo

  2025cites this paper
• Influence of bacterial load on the development and performance of a photoheterotrophic biofilm community for wastewater polishing

  2025cites this paper
• Moderate salt stress aids in the enhancement of nutritional and flavor quality in tomato (Solanum lycopersicum L.) fruits

  2025cites this paper
• Preparation, characterization and anti‑iron deficiency anemia activity of Armillaria mellea polysaccharide-Fe(III) complex.

  2025cites this paper
• Development of a Mixotrophic Strategy for Efficient Cultivation of a Coccolithophore, Chrysotila roscoffensis

  2025cites this paper
• Leaf Plasticity Responses of Four Urban Garden Plants to Low-Light Environments Under Viaducts

  2025cites this paper
• A robust method for quantitative detection of β-1,3-glucan using Limulus polyphemus factor Gα fragment.

  2025cites this paper
• Effects of quercetin on in vitro rumen fermentation parameters, gas production and microflora of beef cattle

  2025cites this paper
• Nutrient-mediated changes in growth, biochemical composition, and biosilica deposition in Cyclotella cryptica

  2025cites this paper
• Structure characterization, antioxidant and β-cell protective effects of the glutaredoxin 2/glutathione system and glutathione metabolism-related Lonicerae Japonicae Caulis pectin.

  2025cites this paper
• Potential function of plant-growth-promoting endophytic Serratia fonticola CPSE11 from Codonopsis pilosula in phytoremediation of Cadmium ion (Cd2+).

  2025cites this paper
• Valorization of Channa striatus waste skin for developing marine collagen peptide based hydrogels for potential wound dressings

  2025cites this paper
• Evaluation of the Concentration of Ethanol as a Vehicle for the Administration of Resveratrol Measured Through Its Antioxidant Effect in the Hippocampus of Wistar Rats

  2025cites this paper
• Ultrasonic-microwave assisted extraction of Auricularia cornea var. Li. polysaccharides: Hydrogel formation, interactions, and antioxidant activity.

  2025cites this paper
• 基于聚丙烯酰胺凝胶电泳结合在线荧光成像的蛋白质特异性定量分析方法

  2025cites this paper
• Acetonitrile-induced modulation of alkaline protease as a biological macromolecule: Micro- and macro-scale effects on catalysis and stability.

  2025cites this paper
• Effect of Grifola frondosa polysaccharide on immune function and gut microbiota in mice

  2025cites this paper
• Effect of medium temperature-activated potassium persulfate pretreatment on sludge anaerobic digestion efficiency and substance transformation processes

  2025cites this paper
• Pharmacological Effect of Water-Extractable (Poly)Phenolic Polysaccharide–Protein Complexes from Prunus spinosa L. Wild Fruits

  2025cites this paper
• Ultrasound‐Assisted Enzymatic Extraction of Pinus pumila Nut Protein: Effects on Yield, Physicochemical and Functional Properties

  2025cites this paper
• Colchicine- and oryzalin-induced hexaploidy enhances nutrient levels and is a promising way to create new germplasms for Chinese chives.

  2025cites this paper
• Unraveling the Role of Leukotriene A4 Hydrolase in Hepatocellular Carcinoma: Implications for Targeted Therapy and Tumor Progression

  2025cites this paper
• Effects of different light intensity on the growth, physiological and biochemical properties, and stomatal ultrastructure of Rhododendron micranthum saplings

  2025cites this paper
• Effects of pectin with distinct structural characteristics on gut microbiota composition and metabolite profiles in naive mice.

  2025cites this paper
• Upstream Proteolysis by Ste24 does not Require a C-Terminal Methyl Ester as Revealed Using 33-Residue a-Factor Precursor Peptide Substrates Synthesized via Epimerization-Free Methods

  2025cites this paper
• Procedures for determining the antimicrobial activity of maggot excretions and secretions of the green bottle fly: a narrative literature review

  2025cites this paper
• Inhibition effect and mechanism of p-tert-butylcatechol on Raphidiopsis raciborskii.

  2025cites this paper
• Molecular Weight–Dependent Pyroptosis Induction by Yeast Mannans Through Dectin‐2/Syk/NLRP3 Axis Enhances Cisplatin Efficacy in Melanoma Therapy

  2025cites this paper
• Insight into the rutin biosynthesis in the unique flavonol synthesis pathway of Tartary buckwheat based on the enzymatic functions of FLSs

  2025cites this paper
• Comparative Study on Growth, Physiological and Biotic Stress Resistance of Cucumber (Cucumis sativus L.) with Jasmonic Acid and Methyl Jasmonate under Greenhouse Conditions

  2025cites this paper
• Regulation of Pseudomonas sp. PSC001 on the Artificial Rumen Environment Contaminated by Zearalenone

  2025cites this paper
• The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection

  2025cites this paper
• Physiological and transcriptomic analysis provides novel insight into growth in Kam sweet rice under different nitrogen supplies

  2024cites this paper
• Role of KOH-activated biochar on promoting anaerobic digestion of biomass from Pennisetumgianteum.

  2024cites this paper
• Oral administration of resveratrol reduces oxidative stress generated in the hippocampus of Wistar rats in response to consumption of ethanol

  2024cites this paper
• Biochar leachate reduces primary nitrogen assimilation by inhibiting nitrogen fixation and microbial nitrate assimilation.

  2024cites this paper
• An in vitro protocol to assay mRNA translation inhibitors using the fluorescent assembly of the split-GFP

  2024cites this paper
• A pectic polysaccharide from Typhonii Rhizoma: Characterization and antiproliferative activity in K562 cells through regulating mitochondrial function and energy metabolism.

  2024cites this paper
• Catalytic properties characterization and degradation mode elucidation of a polyG-specific alginate lyase OUC-FaAly7.

  2024cites this paper
• Knockdown of SlYTHDF2 Accelerates Dark–Induced Tomato Leaf Senescence by Affecting the ABA Pathway

  2024cites this paper
• Effects of salinity and temperature on growth performance, biochemical composition, and biosilification process of Cyclotella cryptica

  2024cites this paper
• Arbuscular mycorrhizal fungi and exogenous Ca2+ application synergistically enhance salt and alkali resistance in perennial ryegrass through diverse adaptive strategies.

  2024cites this paper
• The dietary supplementation of Sargassum fusiforme can effectively alleviate high-fat diet induced metabolic abnormalities

  2024cites this paper
• Neutral oligosaccharides from ginseng (Panax ginseng) residues vs. neutral ginseng polysaccharides: A comparative study of structure elucidation and biological activity.

  2024cites this paper
• Overexpression of SlALC Increases Drought and Salt Tolerance and Affects Fruit Dehiscence in Tomatoes

  2024cites this paper
• Mycorrhizal and non-mycorrhizal perennial ryegrass roots exhibit differential regulation of lipid and Ca2+ signaling pathways in response to low and high temperature stresses.

  2024cites this paper
• Salinity mediates the damage caused by acute and chronic ammonia stress in largemouth bass (Micropterus salmoides).

  2024cites this paper
• Comparative analysis of volatile compounds and functional components in fresh and dried monk fruit (Siraitia grosvenorii)

  2024cites this paper
• Improved the physicochemical properties and bioactivities of oligosaccharides by degrading self-extracting/commercial ginseng polysaccharides.

  2024cites this paper
• Exploring the relationship between pollen viability and inclusion in Paeonia lactiflora after cryopreservation.

  2024cites this paper
• Common structural features in some of the sequentially distant neurotransmitter transporters N-termini.

  2024cites this paper
• A time-course analysis of Aspergillus terreus secretomes reveals the importance of pectin-degrading enzymes to increase the digestibility of soybean meal

  2024cites this paper
• Rational Design, Synthesis, and Computational Investigation of Dihydropyridine [2,3-d] Pyrimidines as Polyphenol Oxidase Inhibitors with Improved Potency

  2024cites this paper
• Phosphorus and selenium compounding mitigates Cr stress in peanut seedlings by enhancing growth homeostasis and antioxidant properties

  2024cites this paper
• Valorizing Arthrospira cell residues into polysaccharides: Characterization and application in yogurt.

  2024cites this paper
• Design and synthesis of a TICT-based red-emissive fluorescent probe for the rapid and selective detection of HSA in human biofluids and live cell imaging.

  2024cites this paper
• Effects of ultrasound treatment on the structure, function properties and in vitro digestion of Sipunculus nudus protein.

  2024cites this paper
• Reduction in Chemical Fertilizer Rates by Applying Bio-Organic Fertilizer for Optimization Yield and Quality of Hemerocallis citrina Baroni

  2024cites this paper
• Exploratory studies of the antidepressant effect of Cordyceps sinensis polysaccharide and its potential mechanism.

  2024cites this paper
• CqNLP1 promotes Chenopodium quinoa Willd. seed germination regulated by NO 3 -

  2024cites this paper
• Evaluation of Bauhinia ungulata Essential Oil as a New Acetylcholinesterase Inhibitor from an in silico and in vitro Perspective in the Northern Amazon of Brazil.

  2024cites this paper
• Expression, Characterization, and Immobilization of a Novel D-Lactate Dehydrogenase from Salinispirillum sp. LH 10-3-1

  2024cites this paper
• NaHS immersion alleviates the stress effect of chromium(III) on alfalfa seeds by affecting active oxygen metabolism

  2024cites this paper