Organic carbon released from tire chips enhances pyrite particle-based autotrophic denitrification of water.

The incorporation of organic matter may enhance the efficacy of mixed-nutrient denitrification. In this study, we explored the effects of waste tire chips on the autotrophic denitrification of pyrite. The synergy between organic carbon and sulfur in mixed-nutrient denitrification resulted in exceptional efficiency. A high tire chip ratio increases the carbon supply to the mixed nutrient denitrification filter, enhances the relative contribution of heterotrophic denitrification, and improves the overall denitrification performance. After an empty bed reaction time (EBRT) of 5 h, the nitrate removal efficiencies of FeS2:Tire filler ratios of 1:0, 2:1, 1:1, and 1:2 were 29.20 %, 54.83 %, 71.13 %, and 76.44 %, respectively. A tire-derived organic carbon source increases the abundance of microbial communities and stimulates the activity of key enzymes involved in nitrogen and sulfur metabolism.

• Publication year

  2025

• Venue

  Journal of Environmental Management

• Publication date

  2025-08-18

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.jenvman.2025.127014PMID 40829229
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A mixotrophic denitrification composite for treatment of nitrate-contaminated water: Performance, microbial community and functional genes

  2024cited by this paper
• Sulfur-carbon loop enhanced efficient nitrogen removal mechanism from iron sulfide-mediated mixotrophic partial denitrification/anammox systems.

  2024cited by this paper
• Impact of organic carbon on sulfide-driven autotrophic denitrification: Insights from isotope fractionation and functional genes.

  2024cited by this paper
• High-rate iron sulfide and sulfur-coupled autotrophic denitrification system: Nutrients removal performance and microbial characterization.

  2023cited by this paper
• Interaction mechanisms of agricultural waste-driven denitrification and V(V) reduction: Internal electron competition, sequential transfer and activation of vanadium

  2023cited by this paper
• Introducing PHBV and controlling the pyrite sizes achieved the pyrite-based mixotrophic denitrification under natural aerobic conditions: Low sulfate production and functional microbe interaction

  2022cited by this paper
• Wastewater reuse for irrigation of produce: A review of research, regulations, and risks.

  2022cited by this paper
• Electron distribution in sulfur-driven autotrophic denitrification under different electron donor and acceptor feeding schemes

  2021cited by this paper
• Effects of micro(nano)plastics on higher plants and the rhizosphere environment.

  2021cited by this paper
• Fe(II) enhances simultaneous phosphorus removal and denitrification in heterotrophic denitrification by chemical precipitation and stimulating denitrifiers activity.

  2021cited by this paper
• Towards a standardized protocol for studying chemolithoautotrophic denitrification with pyrite at circumneutral pH

  2021cited by this paper
• Integrating sulfur, iron(II), and fixed organic carbon for mixotrophic denitrification in a composite filter bed reactor for decentralized wastewater treatment: Performance and microbial community.

  2021cited by this paper
• Specific and effective detection of anammox bacteria using PCR primers targeting the 16S rRNA gene and functional genes.

  2020cited by this paper
• Simultaneous denitrification, phosphorus recovery and low sulfate production in a recirculated pyrite-packed biofilter (RPPB).

  2020cited by this paper
• Response of denitrifying community, denitrification genes and antibiotic resistance genes to oxytetracycline stress in polycaprolactone supported solid-phase denitrification reactor.

  2020cited by this paper
• Assessing the public perceptions of treated wastewater reuse: opportunities and implications for urban communities in developing countries

  2020cited by this paper
• Insight into the mechanism of chemoautotrophic denitrification using pyrite (FeS2) as electron donor.

  2020cited by this paper
• Coupling of sulfur(thiosulfate)-driven denitratation and anammox process to treat nitrate and ammonium contained wastewater.

  2019cited by this paper
• Pilot-scale application of sulfur-limestone autotrophic denitrification biofilter for municipal tailwater treatment: Performance and microbial community structure.

  2019cited by this paper
• Natural pyrite to enhance simultaneous long-term nitrogen and phosphorus removal in constructed wetland: Three years of pilot study.

  2019cited by this paper
• Integrated sulfur- and iron-based autotrophic denitrification process and microbial profiling in an anoxic fluidized-bed membrane bioreactor.

  2019cited by this paper
• Electron donors for autotrophic denitrification

  2019cited by this paper
• Sulfur-siderite autotrophic denitrification system for simultaneous nitrate and phosphate removal: From feasibility to pilot experiments.

  2019cited by this paper
• Simultaneous reduction of perchlorate and nitrate in a combined heterotrophic-sulfur-autotrophic system: Secondary pollution control, pH balance and microbial community analysis.

  2019cited by this paper
• Enrichment and characterization of autotrophic Thiobacillus denitrifiers from anaerobic sludge for nitrate removal

  2018cited by this paper
• Description of Immundisolibacter cernigliae gen. nov., sp. nov., a high-molecular-weight polycyclic aromatic hydrocarbon-degrading bacterium within the class Gammaproteobacteria, and proposal of Immundisolibacterales ord. nov. and Immundisolibacteraceae fam. nov.

  2017cited by this paper
• Nitrite accumulation in continuous-flow partial autotrophic denitrification reactor using sulfide as electron donor.

  2017cited by this paper
• Comparison of particulate pyrite autotrophic denitrification (PPAD) and sulfur oxidizing denitrification (SOD) for treatment of nitrified wastewater.

  2017cited by this paper
• Simultaneous bio-autotrophic reduction of perchlorate and nitrate in a sulfur packed bed reactor: Kinetics and bacterial community structure.

  2017cited by this paper
• Effect of oyster shell medium and organic substrate on the performance of a particulate pyrite autotrophic denitrification (PPAD) process.

  2017cited by this paper
• Fe(II)-mediated autotrophic denitrification: A new bioprocess for iron bioprecipitation/biorecovery and simultaneous treatment of nitrate-containing wastewaters

  2017cited by this paper
• Simultaneous nitrate and phosphate removal from wastewater lacking organic matter through microbial oxidation of pyrrhotite coupled to nitrate reduction.

  2016cited by this paper
• The effects of fulvic acid on microbial denitrification: promotion of NADH generation, electron transfer, and consumption

  2016cited by this paper
• Biological denitrification process based on the Fe(0)-carbon micro-electrolysis for simultaneous ammonia and nitrate removal from low organic carbon water under a microaerobic condition.

  2016cited by this paper
• Soil infiltration bioreactor incorporated with pyrite-based (mixotrophic) denitrification for domestic wastewater treatment.

  2015cited by this paper
• Optimization of C/N and current density in a heterotrophic/biofilm-electrode autotrophic denitrification reactor (HAD-BER).

  2014cited by this paper
• Heterotrophic and elemental-sulfur-based autotrophic denitrification processes for simultaneous nitrate and Cr(VI) reduction.

  2014cited by this paper
• Sulfur-oxidizing autotrophic and mixotrophic denitrification processes for drinking water treatment: elimination of excess sulfate production and alkalinity requirement.

  2012cited by this paper
• Microbial community analysis in the autotrophic denitrification process using spent sulfidic caustic by denaturing gradient gel electrophoresis of PCR-amplified genes.

  2011cited by this paper
• Toxicity assessment of sequential leachates of tire powder using a battery of toxicity tests and toxicity identification evaluations.

  2009cited by this paper
• Biochemistry and molecular biology of lithotrophic sulfur oxidation by taxonomically and ecologically diverse bacteria and archaea.

  2009cited by this paper
• Onsite Wastewater Denitrification Using a Hydrogenotrophic Hollow‐Fiber Membrane Bioreactor

  2009cited by this paper
• Membrane-attached biofilm as a mean to facilitate nitrification in activated sludge process and its effect on the microfaunal population

  2008cited by this paper
• Removal of phosphate species from solution by adsorption onto calcite used as natural adsorbent.

  2007cited by this paper
• Sustainable nitrogen elimination biotechnologies: A review

  2006cited by this paper
• Workgroup Report: Drinking-Water Nitrate and Health—Recent Findings and Research Needs

  2005cited by this paper
• Mitigating nutrient leaching with a sub-surface drainage layer of granulated tires.

  2004cited by this paper
• The sulfane sulfur of persulfides is the actual substrate of the sulfur-oxidizing enzymes from Acidithiobacillus and Acidiphilium spp.

  2003cited by this paper
• Mechanism of sulfide-quinone reductase investigated using site-directed mutagenesis and sulfur analysis.

  2002cited by this paper
• Nitrite Reductase Genes (nirK andnirS) as Functional Markers To Investigate Diversity of Denitrifying Bacteria in Pacific Northwest Marine Sediment Communities

  2000cited by this paper
• Sulfide-Quinone Reductase from Rhodobacter capsulatus: Requirement for Growth, Periplasmic Localization, and Extension of Gene Sequence Analysis

  1999cited by this paper

• No citing papers are available for this paper.