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Metallothionein III Mediates Ca2+-Dependent Zn2+ Spikes to Inhibit Dendritic Arborization

Lyndsie A Salvagio,Chen Zhang,Braden E. Rue,Nicole Doris,Ci Koehring,Isabella Tyler,Raul Vargas,W. Oh,Yan Qin

Published 2025 in Journal of Neuroscience

ABSTRACT

Zinc is crucial for neuron function, but whether and how labile zinc ion (Zn2+) acts as an intracellular signaling molecule remains unclear. In this work, we investigate the relationship between Ca2+ and Zn2+ dynamics using fluorescence imaging. Our findings reveal that manipulating Ca2+ influx through various pathways induces intracellular acidification, which subsequently elicits Zn2+ spikes that reflect transient increases in cytosolic Zn2+ levels. These Ca2+-dependent Zn2+ spikes have been recorded in both rat (Rattus norvegicus) primary neuron cultures and organotypic mouse (Mus musculus) hippocampal slice cultures prepared from both males and females. They are specific to neurons and astrocytes but are absent in other cell types we tested including HeLa cells, COS-7 cells, and fibroblasts. We further identify Metallothionein III (MT3), a Zn2+ buffering protein specifically expressed in brain cells, as the source of these Zn2+ spikes. Reduction in MT3 expression by knockdown with shRNAmiR techniques significantly decreases the amplitude of Zn2+ spikes, while overexpression of MT3 in HeLa and COS-7 cells is sufficient to induce Ca2+-dependent Zn2+ spikes, demonstrating the crucial roles of MT3 in Zn2+ release. Lastly, we explore the biological roles of MT3-mediated Zn2+ spikes in neurons. Suppressing Zn2+ spikes with either MT3 knockdown or mild Zn2+ chelation results in increased dendritic branching in primary rat hippocampal neurons. These results suggest that Zn2+ release from endogenous MT3 acts as a regulatory signal to inhibit dendrite branching and growth, establishing a critical role for Zn2+ spikes in neurite outgrowth and neuronal development.

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