Exploring the Regional Diversity of Eukaryotic Phytoplankton in the English Channel by Combining High‐Throughput Approaches

Monitoring marine phytoplankton is essential to understanding marine ecosystems functioning, especially in productive regions like the English Channel. This study applied high‐throughput sequencing (HTS) and automated pulse shape‐recording flow cytometry (PSR FCM) to investigate the spatial and seasonal variability of phytoplankton diversity in French waters of the English Channel during the ECOPEL cruises in April (spring) and July (summer) 2018. Our findings revealed significant seasonal shifts in size, structure, total red fluorescence (FLR, a biomass proxy) and community composition. PSR FCM provided high‐resolution size class discrimination, revealing an increase in picoeukaryote abundance and lower FLR in summer compared to spring. HTS enabled detailed taxonomic insights: in spring, picoeukaryotes (e.g., Ostreococcus) dominated in the Western English Channel, except in Finistère/Celtic Seas, where microphytoplankton represented the majority of reads. Nanoeukaryotes (Phaeocystis) dominated in the Eastern English Channel. In summer, diversity increased, with co‐dominance of picoeukaryotes (Micromonas, Bathycoccus, Ostreococcus), microphytoplankton (Chaetoceros, Leptocylindrus, Guinardia) and nanoeucaryotes (Teleaulax, Gephyrocapsa) in the Bay of Seine. Beyond a pronounced west‐east disparity, the Bay of Seine exhibited remarkable taxonomic and functional diversity, with high local contribution to beta diversity (LCBD) values in both seasons. Diversity patterns were strongly influenced by temperature and nutrient concentrations (phosphate, nitrogen), with secondary influences from salinity and turbidity. PSR FCM further revealed sub‐mesoscale variability in abundance and size structure, complementing the mesoscale patterns observed through HTS. This study highlights the importance of integrating both methods to capture fine‐scale phytoplankton dynamics and high‐resolution diversity, thereby enhancing ecosystem management, espcecially in nutrient‐sensitive, productive marine regions. The preprint of the manuscript is available on Authorea (DOI: 10.22541/au.174523338.82769110/v1) and relayed by Archimer (https://archimer.ifremer.fr/doc/00951/106275/).

• Publication year

  2025

• Venue

  MicrobiologyOpen

• Publication date

  2025-11-09

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1002/mbo3.70097PMID 41208061PMCID 12597793
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2023influential reference
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  2023cited by this paper
• Feeding by common heterotrophic protists on the mixotrophic dinoflagellate Ansanella granifera (Suessiaceae, Dinophyceae)

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• The importance of niches in defining phytoplankton functional beta diversity during a spring bloom

  2023influential reference
• Evaluation of sequential filtration and centrifugation to capture environmental DNA and survey microbial eukaryotic communities in aquatic environments

  2023cited by this paper
• OUP accepted manuscript

  2022cited by this paper
• Interoperable vocabulary for marine microbial flow cytometry

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• Multiple phytoplankton community responses to environmental change in a temperate coastal system: A trait-based approach

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• Towards quantitative metabarcoding of eukaryotic plankton: an approach to improve 18S rRNA gene copy number bias

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• Harmful Algal Blooms and their impacts on shellfish mariculture follow regionally distinct patterns of water circulation in the western English Channel during the 2018 heatwave.

  2022cited by this paper
• Environmentally induced functional shifts in phytoplankton and their potential consequences for ecosystem functioning

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• Elemental and macromolecular composition of the marine Chloropicophyceae, a major group of oceanic photosynthetic picoeukaryotes

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• Grazing by the Copepod Parvocalanus crassirostris on Picochlorum sp. at Harmful Bloom Densities and the Role of Particle Size

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• Phytoplankton distribution from Western to Central English Channel, revealed by automated flow cytometry during the summer-fall transition

  2020influential reference
• Microscopy and phylogeny of Pyramimonas tatianae sp. nov. (Pyramimonadales, Chlorophyta), a scaly quadriflagellate from Golden Horn Bay (eastern Russia) and formal description of Pyramimonadophyceae classis nova

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• Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea

  2020influential reference
• Three decades of data on phytoplankton and phycotoxins on the French coast: Lessons from REPHY and REPHYTOX.

  2020cited by this paper
• Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer

  2019cited by this paper
• Changes in marine phytoplankton diversity: Assessment under the Marine Strategy Framework Directive

  2019cited by this paper
• Global pattern of phytoplankton diversity driven by temperature and environmental variability

  2019cited by this paper
• Weekly dynamics of abundance and size structure of specific nanophytoplankton lineages in coastal waters (Baltic Sea)

  2019cited by this paper
• Marked changes in diversity and relative activity of picoeukaryotes with depth in the global ocean

  2019cited by this paper
• The New Tree of Eukaryotes.

  2019cited by this paper
• Disentangling the correlates of species and site contributions to beta diversity in dung beetle assemblages

  2018cited by this paper
• Re‐investigation of Gymnodinium natalense (Dinophyceae), a tidal pool dinoflagellate from South Africa and the proposal of a new combination Ansanella natalensis

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• Bolidophyceae, a Sister Picoplanktonic Group of Diatoms – A Review

  2018cited by this paper
• Phytoplankton defence mechanisms: traits and trade‐offs

  2018cited by this paper
• Realized niche analysis of phytoplankton communities involving HAB: Phaeocystis spp. as a case study.

  2018cited by this paper
• Mantoniella Beaufortii and Mantoniella Baffinensis sp. nov. (Mamiellales, Mamiellophyceae), Two New Green Algal Species from the High Arctic

  2018cited by this paper
• Sizes of the centric diatom Actinocyclus normanii as salinity function, a new tool for the assessment of paleoenvironments

  2018cited by this paper
• Monitoring natural phytoplankton communities: a comparison between traditional methods and pulse-shape recording flow cytometry

  2017cited by this paper
• Haptophyte Diversity and Vertical Distribution Explored by 18S and 28S Ribosomal RNA Gene Metabarcoding and Scanning Electron Microscopy

  2017cited by this paper
• Ardissonea crystallina has a type of sexual reproduction that is unusual for centric diatoms

  2017cited by this paper
• Functional trait variation and nitrogen use efficiency in temperate coastal phytoplankton

  2017cited by this paper
• Do marine phytoplankton follow Bergmann's rule sensu lato?

  2017cited by this paper
• Chloropicophyceae, a new class of picophytoplanktonic prasinophytes

  2017cited by this paper
• Nutrient ratios influence variability in Pseudo-nitzschia species diversity and particulate domoic acid production in the Bay of Seine (France).

  2017cited by this paper
• Exploring species and site contributions to beta diversity in stream insect assemblages

  2016influential reference
• Diversity and distribution of haptophytes revealed by environmental sequencing and metabarcoding – a review

  2016cited by this paper
• Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii1[OPEN]

  2016cited by this paper
• Functional Traits

  2016cited by this paper
• Eutrophication modelling chain for improved management strategies to prevent algal blooms in the Bay of Seine

  2016cited by this paper
• Small-scale variability of protistan planktonic communities relative to environmental pressures and biotic interactions at two adjacent coastal stations

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• Spatio-temporal patterns in phytoplankton assemblages in inshore–offshore gradients using flow cytometry: A case study in the eastern English Channel

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• Is metabarcoding suitable for estuarine plankton monitoring? A comparative study with microscopy

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• Using community metabolomics as a new approach to discriminate marine microbial particulate organic matter in the western English Channel

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• Cell size as a key determinant of phytoplankton metabolism and community structure.

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• Growth and diel vertical migration patterns of the toxic dinoflagellate Protoceratium reticulatum in a water column with salinity stratification: the role of bioconvection and light

  2015cited by this paper
• Nanoplankton and picoplankton in the Western English Channel: abundance and seasonality from 2007–2013

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• Free-living marine planktonic unarmoured dinoflagellates from the Gulf of Mexico and the Mexican Pacific

  2015cited by this paper
• High spatial variability of phytoplankton assessed by flow cytometry, in a dynamic productive coastal area, in spring:the eastern English Channel

  2015cited by this paper
• Seasonal variations of marine protist community structure based on taxon-specific traits using the eastern English Channel as a model coastal system.

  2015cited by this paper
• High-resolution analysis of a North Sea phytoplankton community structure based on in situ flow cytometry observations and potential implication for remote sensing

  2015cited by this paper
• Interactions between temperature and nutrients across levels of ecological organization

  2015cited by this paper
• Global patterns in phytoplankton community size structure—evidence for a direct temperature effect

  2014cited by this paper
• Feeding by the newly described mixotrophic dinoflagellate Gymnodinium smaydae: Feeding mechanism, prey species, and effect of prey concentration

  2014cited by this paper
• Phytoplankton Diversity and Community Composition along the Estuarine Gradient of a Temperate Macrotidal Ecosystem: Combined Morphological and Molecular Approaches

  2014cited by this paper
• Temporal changes in the phytoplankton community along the French coast of the eastern English Channel and the southern Bight of the North Sea

  2014cited by this paper
• Dynamics of phytoplankton diversity structure and primary productivity in the English Channel

  2014cited by this paper
• Beta diversity as the variance of community data: dissimilarity coefficients and partitioning.

  2013cited by this paper
• Size and biomechanic properties of diatom frustules influence food uptake by copepods

  2013cited by this paper
• Study of dynamics of phytoplankton and photosynthetic parameters using opportunity ships in the western English Channel

  2013cited by this paper
• Characterization of Phaeocystis globosa (Prymnesiophyceae), the blooming species in the Southern North Sea

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• Phytoplankton primary production in the world's estuarine-coastal ecosystems

  2013cited by this paper
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• Functional traits explain phytoplankton community structure and seasonal dynamics in a marine ecosystem.

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• A global diatom database – abundance, biovolume and biomass in the world ocean

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• Are the eastern and western basins of the English Channel two separate ecosystems?

  2012influential reference

• Multiscale phytoplankton dynamics in a coastal system of the eastern English Channel: the Boulogne-sur-Mer coastal area

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• Phytoplankton coastal-offshore monitoring by the Strait of Dover at high spatial resolution: the DYPHYRAD surveys – Reply to Referee Report

  year unknowninfluential citation