Global genome analysis identifies glycolipids and lipoteichoic acid alanylation as contributors to group B streptococcal diabetic wound infection.

Diabetic individuals frequently develop chronic, hyper-inflammatory, non-healing wounds, which are the leading cause of all non-traumatic amputations. Group B Streptococcus (GBS) is a prominent bacterium isolated from diabetic wound infections, and in a murine model of diabetic wound infection, GBS stimulates an influx of neutrophils into the wound. Utilizing a transposon mutant screen, we identified 291 bacterial genes required for fitness during diabetic wound infection, including enzymes involved in glycolipid biosynthesis and lipoteichoic acid (LTA) alanylation. GBS mutants lacking either LTA alanylation (ΔdltA) or all glycolipids (ΔiagB) are attenuated in murine diabetic wound infection. GBS induces primary and secondary degranulation in primary human neutrophils, and the ΔiagB mutant is significantly more susceptible to neutrophil killing by cationic antimicrobial peptides and reactive oxygen species than ΔdltA. Finally, we found that depletion of neutrophils led to significantly greater bacterial recovery, highlighting the importance of neutrophil defense during diabetic wound infection.

• Publication year

  2025

• Venue

  Cell Reports

• Publication date

  2025-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.celrep.2025.116510PMID 41191485PMCID PMC12664321
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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