Simulating mono- and multiprotein phosphorylation within nanoclusters.

Protein nanoclustering is a characteristic feature of their activated state and is essential for forming numerous subcellular structures. The formation of these nanoclusters is highly dependent on a series of posttranslational modifications, such as mono- and multiphosphorylation and dephosphorylation of residues. We theoretically simulate how a protein can be either mono- or multiphosphorylated on several residues in functional nanoclusters, depending on effective biophysical parameters (diffusion, dwell time, etc.). Moving beyond a binary view of phosphorylation, this approach highlights the interplay between mono- and multiphosphorylation, the cooperative effects generally associated with multiphosphorylation networks, and stresses the role of phosphatases in transforming graded phosphorylation signals into almost switchlike responses. The results are discussed in light of experiments that probe the distribution of phospho-residues.

• Publication year

  2025

• Venue

  Physical Review E

• Publication date

  2025-11-12

• Fields of study

  Biology, Medicine, Physics, Chemistry

• Identifiers
  DOI 10.1103/bb12-kz72arXiv 2511.09295PMID 41560242
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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