Diverse Bacterial PKS Sequences Derived From Okadaic Acid-Producing Dinoflagellates

Okadaic acid (OA) and the related dinophysistoxins are isolated from dinoflagellates of the genus Prorocentrum and Dinophysis. Bacteria of the Roseobacter group have been associated with okadaic acid producing dinoflagellates and have been previously implicated in OA production. Analysis of 16S rRNA libraries reveals that Roseobacter are the most abundant bacteria associated with OA producing dinoflagellates of the genus Prorocentrum and are not found in association with non-toxic dinoflagellates. While some polyketide synthase (PKS) genes form a highly supported Prorocentrum clade, most appear to be bacterial, but unrelated to Roseobacter or Alpha-Proteobacterial PKSs or those derived from other Alveolates Karenia brevis or Crytosporidium parvum.

• Publication year

  2008

• Venue

  Marine Drugs

• Publication date

  2008-03-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/md20080009PMID 18728765PMCID 2525486
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Multiple simultaneous detection of Harmful Algal Blooms (HABs) through a high throughput bead array technology, with potential use in phytoplankton community analysis.

  2009cited by this paper
• Advances in polyketide synthase structure and function.

  2008cited by this paper
• Molecular Evolution and Phylogenetics

  2008cited by this paper
• Bacteria of the Roseobacter Clade Show Potential for Secondary Metabolite Production

  2007cited by this paper
• New targets in diarrhetic shellfish poisoning control.

  2005cited by this paper
• Discovery of a novel superfamily of type III polyketide synthases in Aspergillus oryzae.

  2005cited by this paper
• A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates.

  2005cited by this paper
• Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis.

  2005cited by this paper
• Discovery of okadaic acid esters in the toxic dinoflagellate Dinophysis acuta from New Zealand using liquid chromatography/tandem mass spectrometry.

  2004cited by this paper
• The dinoflagellate genus Prorocentrum along the coasts of the Mexican Pacific

  2004cited by this paper
• PAUP* [Phylogenetic Analysis Using Parsimony (and Other Methods)]

  2004cited by this paper
• Manual on Harmful Marine Microalgae

  2003cited by this paper
• Polyketide Synthase Genes from Marine Dinoflagellates

  2003cited by this paper
• Studies of the biosynthesis of DTX-5a and DTX-5b by the dinoflagellate Prorocentrum maculosum: regiospecificity of the putative Baeyer-Villigerase and insertion of a single amino acid in a polyketide chain.

  2003cited by this paper
• Identification of new okadaic acid derivatives from laboratory cultures of Prorocentrum lima.

  2003cited by this paper
• Marine Biotoxins of Algal Origin and Seafood Safety

  2003cited by this paper
• MORPHOLOGY AND TAXONOMY OF PROROCENTRUM MEXICANUM AND REINSTATEMENT OF PROROCENTRUM RHATHYMUM (DINOPHYCEAE) 1

  2003cited by this paper
• Okadaic acid: the archetypal serine/threonine protein phosphatase inhibitor.

  2002influential reference
• PAUP*: Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10

  2002cited by this paper
• Cryptosporidium parvum: the first protist known to encode a putative polyketide synthase.

  2002cited by this paper
• IDENTIFICATION OF BACTERIA ASSOCIATED WITH DINOFLAGELLATES (DINOPHYCEAE) ALEXANDRIUM SPP. USING TYRAMIDE SIGNAL AMPLIFICATION–FLUORESCENT IN SITU HYBRIDIZATION AND CONFOCAL MICROSCOPY1

  2002cited by this paper
• Relationships between intracellular bacteria and the bivalve killer dinoflagellate Heterocapsa circularisquama (Dinophyceae)

  2001cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Isolation and structural determination of DTX-6, a new okadaic acid derivative.

  2001cited by this paper
• Okadaic acid production from the marine benthic dinoflagellate Prorocentrum arenarium Faust (Dinophyceae) isolated from Europa Island coral reef ecosystem (SW Indian Ocean).

  2000cited by this paper
• PAUP* Phylogenetic analysis using parsimony (*and other methods) Version 4

  2000cited by this paper
• Isolation of a new okadaic acid analogue from phytoplankton implicated in diarrhetic shellfish poisoning.

  1998cited by this paper
• MODELTEST: testing the model of DNA substitution

  1998influential reference
• Morphology and Toxicology of Prorocentrum faustiae sp. nov., a Toxic Species of Non-Planktonic Dinoflagellate from Heron Island, Australia

  1998cited by this paper
• Okadaic acid production from the marine dinoflagellate Prorocentrum belizeanum Faust isolated from the Belizean coral reef ecosystem.

  1998cited by this paper
• Bacteria of the Genus Roseobacter Associated with the Toxic Dinoflagellate Prorocentrum lima.

  1998cited by this paper
• The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.

  1997cited by this paper
• Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

  1997cited by this paper
• A protein phosphatase 2A inhibition assay for a fast and sensitive assessment of okadaic acid contamination in mussels.

  1996cited by this paper
• Biosynthesis of DTX-4: Confirmation of a Polyketide Pathway, Proof of a Baeyer−Villiger Oxidation Step, and Evidence for an Unusual Carbon Deletion Process

  1996cited by this paper
• The toxic dinoflagellate Prorocentrum lima and its associated bacteria: I. An ultrastructural study

  1995cited by this paper
• Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima.

  1995cited by this paper
• Biosynthetic origin of C-37 and C-38 in the polyether toxins okadaic acid and dinophysistoxin-1

  1994cited by this paper
• Highly sensitive assay of okadaic acid using protein phosphatase and paranitrophenyl phosphate.

  1994influential reference
• Interactions entre un dinoflagellé toxique et sa microflore bactérienne associée: rôle des bactéries dans la toxicité de Prorocentrum lima Ehrenberg (Dodge)

  1993cited by this paper
• Ultrastructure of two toxic marine dinoflagellates, Prorocentrum lima and Prorocentrum maculosum

  1993cited by this paper
• New diol esters isolated from cultures of the dinoflagellates prorocentrum lima and prorocentrum concavum

  1992cited by this paper
• Identification of okadaic acid from a Caribbean dinoflagellate, Prorocentrum concavum.

  1990cited by this paper
• MORPHOLOGIC DETAILS OF SIX BENTHIC SPECIES OF PROROCENTRUM (PYRROPHYTA) FROM A MANGROVE ISLAND, TWIN CAYS, BELIZE, INCLUDING TWO NEW SPECIES 1

  1990cited by this paper
• THE FINE STRUCTURE OF TWO PHOTOSYNTHETIC SPECIES OF DINOPHYSIS (DINOPHYSIALES, DINOPHYCEAE) 1

  1990cited by this paper
• Bacteria Associated with a Photosynthetic Dinoflagellate in Culture

  1990cited by this paper
• TOXINS PRODUCED BY BENTHIC DINOFLAGELLATES

  1987cited by this paper
• CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP

  1985cited by this paper
• 渦鞭毛藻Prorocentrum limaの毒の主成分PL toxin-IIの単離と同定

  1982cited by this paper
• Isolation and structural elucidation of the causative toxin of the diarrhetic shellfish poisoning.

  1982cited by this paper
• Acanthifolicin, a new episulfide-containing polyether carboxylic acid from extracts of the marine sponge Pandaros acanthifolium

  1981cited by this paper
• Okadaic acid, a cytotoxic polyether from two marine sponges of the genus Halichondria

  1981cited by this paper
• On dinoflagellate evolution.

  1980cited by this paper
• Culture of Phytoplankton for Feeding Marine Invertebrates

  1975influential reference
• Culture methods.

  1967cited by this paper
• SOME CONSIDERATIONS OF THE GROWTH OF MARINE ALGAE IN ARTIFICIAL MEDIA.

  1964cited by this paper

• Multi-omics analysis reveals potential mechanisms of diarrhetic shellfish toxin and fatty acid synthesis in marine harmful Prorocentrum.

  2025cites this paper
• Functional diversity of bacterial microbiota associated with the toxigenic benthic dinoflagellate Prorocentrum

  2024cites this paper
• Microbiome Associated With Gambierdiscus balechii Cultures Under Different Toxicity Conditions

  2022cites this paper
• High Value Phycotoxins From the Dinoflagellate Prorocentrum

  2021cites this paper
• Associated Bacteria and Their Effects on Growth and Toxigenicity of the Dinoflagellate Prorocentrum lima Species Complex From Epibenthic Substrates Along Mexican Coasts

  2020cites this paper
• Nontarget screening and toxicity evaluation of diol esters of okadaic acid and dinophysistoxins reveal intraspecies difference of Prorocentrum lima.

  2020cites this paper
• Paralytic shellfish toxin producing Aphanizomenon gracile strains isolated from Lake Iznik, Turkey

  2018cites this paper
• Marine microorganisms as a promising and sustainable source of bioactive molecules.

  2017cites this paper
• Comparative Transcriptome Analysis of Four Prymnesiophyte Algae

  2014cites this paper
• Evaluating the performance of sampling plans for phycotoxins in shellfish: Improvement of an existing method

  2014cites this paper
• Toxigenic Pfiesteria species—Updates on biology, ecology, toxins, and impacts

  2012cites this paper
• Bioactives from microalgal dinoflagellates.

  2012cites this paper
• Biosynthetic Studies on Water-Soluble Derivative 5c (DTX5c)

  2012cites this paper
• Biomimetic Synthesis of Polyether Natural Products via Polyepoxide Opening

  2011cites this paper
• Biosynthesis and Molecular Genetics of Polyketides in Marine Dinoflagellates

  2010influential citation
• Identification of okadaic acid production in the marine dinoflagellate Prorocentrum rhathymum from Florida Bay.

  2010cites this paper
• Synthesis of Marine Polycyclic Polyethers via Endo-Selective Epoxide-Opening Cascades

  2010cites this paper
• Biosynthesis of toxic naturally-occurring seafood contaminants.

  2010cites this paper
• Prymnesins: Toxic Metabolites of the Golden Alga, Prymnesium parvum Carter (Haptophyta)

  2010cites this paper
• Special Issue on Marine Toxins

  2009influential citation
• Absolute configuration and biosynthesis of pahayokolide A from Lyngbya sp. Strain 15-2 of the Florida Everglades

  2009cites this paper
• Epoxide-opening cascades in the synthesis of polycyclic polyether natural products.

  2009cites this paper
• Epoxidöffnungskaskaden zur Synthese polycyclischer Polyether-Naturstoffe

  2009cites this paper
• MIT Open Access Articles Synthesis of Marine Polycyclic Polyethers via Endo-Selective Epoxide-Opening Cascades

  year unknowncites this paper