Lithium Chloride rescues Monensin-potentiated Wnt signaling inhibition in inflammatory bone loss in rats

Wnt signaling has been related to inflammatory bone loss. The study aimed to evaluate the effect of Lithium Chloride (LiCl) on animals submitted to Monensin-potentiated periodontal bone loss. For that, Wistar rats were submitted to experimental periodontitis (EP) and received either 0.1ml/200g corn oil (vehicle) daily; or Monensin (Mon), receiving 10 mg/kg of Mon daily; or LiCl, receiving 150mg/kg of LiCl on alternate days; or Mon+LiCl. The Naïve (N) group was not submitted to any treatment. After euthanasia, maxillae were collected for macroscopic and histological analyses, and bone markers and Wnt signaling were expressed. Molecular docking assays were used to assess the affinity between Mon and WNT pathway components. It was seen that EP caused bone loss and inflammation in the periodontium, which was potentiated by Mon. LiCl protected bone and rescued the deleterious effects of Mon marked by a reduction in bone loss, an increase in osteoblasts number (39%), and Runx2 (70%) and OPG (68%) gene expressions, concomitant with a reduction of osteoclasts in number (32%) and function (27%). LiCL increased beta-catenin's gene and protein expressions in animals with EP receiving Mon. In summary, LiCl rescued the deleterious effect of Mon on bone in animals with EP and stands as a pharmacological tool to counteract the potent inhibition of the Wnt pathway.

• Publication year

  2025

• Venue

  Brazilian Dental Journal

• Publication date

  2025-11-21

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1590/0103-644020256475PMID 41294202PMCID 12641581
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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