Research insights and clinical opportunities of functional organoid-based platforms

Stem cell- or tissue-derived three-dimensional organ-like formations, known as organoids, are emerging as effective tools in biomedicine. Since they may be useful in developing customized therapeutic solutions and efficient drug screening protocols, organoids can deepen our understanding of novel disease mechanisms. In doing so, they can facilitate advancements in drug discovery platforms, pharmacological safety, and clinical trials. This review explores various biomedical applications of organoids, including drug development and disease modeling, and highlights specific tools and analytical techniques that can be employed to investigate organoids and their microenvironments. Finally, we review recent clinical trials and patents related to organoids that show great promise for future clinical translation.

• Publication year

  2025

• Venue

  Bio-Design and Manufacturing

• Publication date

  2025-11-01

• Fields of study

  Not labeled

• Identifiers
  DOI 10.1631/bdm.2400519
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Human fetal brain self-organizes into long-term expanding organoids.

  2024cited by this paper
• Acoustic virtual 3D scaffold for direct-interacting tumor organoid–immune cell coculture systems

  2024cited by this paper
• A Phase III Randomized Trial of Integrated Genomics and Avatar Models for Personalized Treatment of Pancreatic Cancer: The AVATAR Trial

  2024cited by this paper
• Genetically modified organoids for tissue engineering and regenerative medicine.

  2024cited by this paper
• A prospective study of neoadjuvant pembrolizumab plus chemotherapy for resectable esophageal squamous cell carcinoma: The Keystone-001 trial.

  2024cited by this paper
• Lessons Learned From Clinical Trials of CRT: What We Need to Know.

  2024cited by this paper
• Role of Wnt5a in modulation of osteoporotic adipose‐derived stem cells and osteogenesis

  2024cited by this paper
• Abnormal Cellular Populations Shape Thymic Epithelial Tumor Heterogeneity and Anti‐Tumor by Blocking Metabolic Interactions in Organoids

  2024cited by this paper
• Functional status and quality of life in older patients with advanced esophageal squamous cell cancer receiving second-line nivolumab ± ipilimumab therapy: A post hoc analysis of the phase 2, multicenter RAMONA study.

  2024cited by this paper
• Cancer organoids 2.0: modelling the complexity of the tumour immune microenvironment

  2024cited by this paper
• Integrating 3D Bioprinting and Organoids to Better Recapitulate the Complexity of Cellular Microenvironments for Tissue Engineering

  2024cited by this paper
• Identification and validation of tumor-specific T cell receptors from tumor infiltrating lymphocytes using tumor organoid co-cultures

  2024cited by this paper
• A Decade of Organoid Research: Progress and Challenges in the Field of Organoid Technology

  2024cited by this paper
• Revitalizing liver function in mice with liver failure through transplantation of 3D-bioprinted liver with expanded primary hepatocytes

  2024cited by this paper
• Organoid bioinks: construction and application

  2024cited by this paper
• An integrated view of cisplatin-induced nephrotoxicity, hepatotoxicity, and cardiotoxicity: characteristics, common molecular mechanisms, and current clinical management

  2024cited by this paper
• Clinical, experimental and pathophysiological effects of Yaq-001: a non-absorbable, gut-restricted adsorbent in models and patients with cirrhosis

  2024cited by this paper
• Derivation of functional thymic epithelial organoid lines from adult murine thymus.

  2024cited by this paper
• Closing in on cancer heterogeneity with organoids

  2024cited by this paper
• Circulating microRNA analysis in a prospective co-clinical trial identifies MIR652-3p as a response biomarker and driver of regorafenib resistance mechanisms in colorectal cancer.

  2024cited by this paper
• Engineering the 3D structure of organoids

  2024cited by this paper
• Biomaterial-assisted organoid technology for disease modeling and drug screening

  2024cited by this paper
• Long-term in vitro expansion of a human fetal pancreas stem cell that generates all three pancreatic cell lineages.

  2024cited by this paper
• A dual-functional microfluidic chip for guiding personalized lung cancer medicine: combining EGFR mutation detection and organoid-based drug response test.

  2024cited by this paper
• Alveolar Organoids in Lung Disease Modeling

  2024cited by this paper
• Xenotransplantation: Current Challenges and Emerging Solutions

  2023cited by this paper
• Advances in Microfluidic Technologies in Organoid Research

  2023cited by this paper
• Cancer organoid-based diagnosis reactivity prediction (CODRP) index-based anticancer drug sensitivity test in ALK-rearrangement positive non-small cell lung cancer (NSCLC)

  2023cited by this paper
• Recent advances in lung organoid development and applications in disease modeling

  2023cited by this paper
• Engineered organoids for biomedical applications.

  2023cited by this paper
• Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application

  2023cited by this paper
• Assessment of doxorubicin toxicity using human cardiac organoids: A novel model for evaluating drug cardiotoxicity.

  2023cited by this paper
• Design and realization of lung organoid cultures for COVID-19 applications

  2023cited by this paper
• Cytotoxic innate intraepithelial lymphocytes control early stages of Cryptosporidium infection

  2023cited by this paper
• Low-dose of polystyrene microplastics induce cardiotoxicity in mice and human-originated cardiac organoids.

  2023cited by this paper
• Drug repurposing screening and mechanism analysis based on human colorectal cancer organoids

  2023cited by this paper
• Advanced human iPSC-based preclinical model for Parkinson's disease with optogenetic alpha-synuclein aggregation.

  2023cited by this paper
• Organoid cultures for cancer modeling.

  2023cited by this paper
• A review on current brain organoid technologies from a biomedical engineering perspective.

  2023cited by this paper
• Organoids: The current status and biomedical applications

  2023cited by this paper
• Guidance of adjuvant instillation in intermediate-risk non-muscle invasive bladder cancer by drug screens in patient derived organoids: a single center, open-label, phase II trial

  2023cited by this paper
• Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells

  2023cited by this paper
• Precision medicine applied to metastatic colorectal cancer using tumor-derived organoids and in-vitro sensitivity testing: a phase 2, single-center, open-label, and non-comparative study

  2023cited by this paper
• Stomach-derived human insulin-secreting organoids restore glucose homeostasis

  2023cited by this paper
• Subcutaneous Infliximab Monotherapy Versus Combination Therapy with Immunosuppressants in Inflammatory Bowel Disease: A Post Hoc Analysis of a Randomised Clinical Trial

  2023cited by this paper
• Generation of functional thymic organoids from human pluripotent stem cells

  2023cited by this paper
• Study on drug screening multicellular model for colorectal cancer constructed by three-dimensional bioprinting technology

  2023cited by this paper
• Combined PD-1, BRAF and MEK inhibition in BRAFV600E colorectal cancer: a phase 2 trial

  2023cited by this paper
• Organoids revealed: morphological analysis of the profound next generation in-vitro model with artificial intelligence

  2023cited by this paper
• Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity

  2022cited by this paper
• Recapitulating hepatitis E virus–host interactions and facilitating antiviral drug discovery in human liver–derived organoids

  2022cited by this paper
• Type-I-interferon signaling drives microglial dysfunction and senescence in human iPSC models of Down syndrome and Alzheimer's disease.

  2022cited by this paper
• Patient-specific modeling of stroma-mediated chemoresistance of pancreatic cancer using a three-dimensional organoid-fibroblast co-culture system

  2022cited by this paper
• Progress in xenotransplantation: overcoming immune barriers

  2022cited by this paper
• The landscape of aging

  2022cited by this paper
• De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids

  2022cited by this paper
• Single-cell genomic and transcriptomic landscapes of primary and metastatic colorectal cancer tumors

  2022cited by this paper
• Downregulation of DNA methyltransferase-3a ameliorates the osteogenic differentiation ability of adipose-derived stem cells in diabetic osteoporosis via Wnt/β-catenin signaling pathway

  2022cited by this paper
• Organoids: a systematic review of ethical issues

  2022cited by this paper
• Organoids in gastrointestinal diseases: from experimental models to clinical translation

  2022cited by this paper
• Assay establishment and validation of a high-throughput organoid-based drug screening platform

  2022cited by this paper
• Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis

  2022cited by this paper
• Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research

  2021cited by this paper
• Modeling Sporadic Alzheimer's Disease in Human Brain Organoids under Serum Exposure

  2021cited by this paper
• Next Generation in vitro Tumor Models Guiding Cancer Therapy.

  2021cited by this paper
• Patient-Derived Organoid Models of Human Neuroendocrine Carcinoma

  2021cited by this paper
• Human kidney organoids reveal the role of glutathione in Fabry disease

  2021cited by this paper
• Cardioids reveal self-organizing principles of human cardiogenesis

  2020cited by this paper
• Snake Venom Gland Organoids.

  2020cited by this paper
• Commercialization of Organoids.

  2020cited by this paper
• Drug compound screening in single and integrated multi-organoid body-on-a-chip systems

  2020cited by this paper
• [Organoids: new perspectives and ethical issues].

  2020cited by this paper
• Human cardiac organoids for the modelling of myocardial infarction and drug cardiotoxicity

  2020cited by this paper
• Generation and Characterization of Patient-Derived Head and Neck, Oral, and Esophageal Cancer Organoids.

  2020cited by this paper
• Modeling Human Cytomegalovirus-Induced Microcephaly in Human iPSC-Derived Brain Organoids

  2020cited by this paper
• Heterogeneity

  2020cited by this paper
• Antitumor Activity of Amivantamab (JNJ-61186372), an EGFR-cMet Bispecific Antibody, in Diverse Models of EGFR Exon 20 Insertion-Driven NSCLC.

  2020cited by this paper
• A brief history of organoids

  2020cited by this paper
• COVID-19: organoids go viral

  2020cited by this paper
• Characterization of Human Colon Organoids From Inflammatory Bowel Disease Patients

  2020cited by this paper
• Abstract CT111: Naproxen chemoprevention promotes immune activation in Lynch syndrome colorectal mucosa

  2020cited by this paper
• Intrinsic Endocardial Defects Contribute to Hypoplastic Left Heart Syndrome.

  2020cited by this paper
• SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids

  2020cited by this paper
• Organoid culture system for patient-derived lung metastatic osteosarcoma

  2020cited by this paper
• Identification of SARS-CoV-2 Inhibitors using Lung and Colonic Organoids

  2020cited by this paper
• Rethinking organoid technology through bioengineering

  2020cited by this paper
• EGFR amplification and outcome in a randomised phase III trial of chemotherapy alone or chemotherapy plus panitumumab for advanced gastro-oesophageal cancers

  2020cited by this paper
• Progenitor identification and SARS-CoV-2 infection in human distal lung organoids

  2020cited by this paper
• The potential toxic effects of magnesium oxide nanoparticles and valproate on liver tissue

  2020cited by this paper
• An Automated Organoid Platform with Inter-organoid Homogeneity and Inter-patient Heterogeneity

  2020cited by this paper
• A rectal cancer organoid platform to study individual responses to chemoradiation

  2019cited by this paper
• Emerging organoid models: leaping forward in cancer research

  2019cited by this paper
• Tumor organoid–T-cell coculture systems

  2019cited by this paper
• Patient-Derived Organoids Predict Chemoradiation Responses of Locally Advanced Rectal Cancer.

  2019cited by this paper
• Organoid Cultures as Preclinical Models of Non–Small Cell Lung Cancer

  2019cited by this paper
• Organoids reveal that inherent radiosensitivity of small and large intestinal stem cells determines organ sensitivity.

  2019cited by this paper
• Development of organoid-based drug metabolism model.

  2019cited by this paper
• HER2 exon 20 insertions in non-small-cell lung cancer are sensitive to the irreversible pan-HER receptor tyrosine kinase inhibitor pyrotinib

  2019cited by this paper
• Breakthrough Moments: Yoshiki Sasai's Discoveries in the Third Dimension.

  2019cited by this paper
• Patient-Derived Cancer Organoid Cultures to Predict Sensitivity to Chemotherapy and Radiation

  2019cited by this paper

• No citing papers are available for this paper.