A conserved flagella-associated protein in Chlamydomonas, FAP234, is essential for axonemal localization of tubulin polyglutamylase TTLL9

A novel axonemal protein, FAP234, of Chlamydomonas is found to form a complex with a tubulin-polyglutamylating enzyme, TTLL9, and function in the stabilization and intraflagellar transport of TTLL9. These proteins are conserved in most ciliated organisms and may be specialized for regulation of ciliary motility.

• Publication year

  2014

• Venue

  Molecular Biology of the Cell

• Publication date

  2014-01-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1091/mbc.E13-07-0424PMID 24196831PMCID 3873882
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dynein and intraflagellar transport.

  2015cited by this paper
• A Role for the Membrane in Regulating Chlamydomonas Flagellar Length

  2013cited by this paper
• Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase

  2013cited by this paper
• Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner‐arm dynein that interacts with the dynein regulatory complex

  2012cited by this paper
• 14 – Dynein and Intraflagellar Transport

  2012cited by this paper
• Ciliary and flagellar structure and function--their regulations by posttranslational modifications of axonemal tubulin.

  2012cited by this paper
• CEP41 is mutated in Joubert syndrome and is required for tubulin glutamylation at the cilium

  2011influential reference
• Tubulin Tyrosine Ligase-like Genes ttll3 and ttll6 Maintain Zebrafish Cilia Structure and Motility*

  2011cited by this paper
• Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions

  2011cited by this paper
• CPP-1 Regulates the Function and Stability of Sensory Cilia in C . elegans

  2011cited by this paper
• A family of protein-deglutamylating enzymes associated with neurodegeneration.

  2010cited by this paper
• Axonemal Motility by Modulating Activities of Inner-Arm Dyneins

  2010influential reference
• Chlamydomonas IFT70/CrDYF-1 Is a Core Component of IFT Particle Complex B and Is Required for Flagellar Assembly

  2010influential reference
• Tubulin glutamylation regulates ciliary motility by altering inner dynein arm activity.

  2010cited by this paper
• Tubulin polyglutamylation is essential for airway ciliary function through the regulation of beating asymmetry

  2010cited by this paper
• Identification of Tubulin Deglutamylase among Caenorhabditis elegans and Mammalian Cytosolic Carboxypeptidases (CCPs)*♦

  2010cited by this paper
• The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella

  2009cited by this paper
• Hyperglutamylation of Tubulin Can either Stabilize or Destabilize Microtubules in the Same Cell

  2009cited by this paper
• The Chlamydomonas sourcebook

  2009cited by this paper
• Polyglutamylation: a fine‐regulator of protein function?

  2008cited by this paper
• The expanding field of poly(ADP-ribosyl)ation reactions. ‘Protein Modifications: Beyond the Usual Suspects' Review Series

  2008cited by this paper
• A targeted multienzyme mechanism for selective microtubule polyglutamylation.

  2007influential reference
• Loss of α-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function

  2007cited by this paper
• The zebrafish fleer gene encodes an essential regulator of cilia tubulin polyglutamylation.

  2007cited by this paper
• The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions

  2007cited by this paper
• Axonemal localization of Chlamydomonas PACRG, a homologue of the human Parkin-coregulated gene product.

  2007cited by this paper
• Tubulin Polyglutamylase Enzymes Are Members of the TTL Domain Protein Family

  2005cited by this paper
• Proteomic analysis of a eukaryotic cilium

  2005influential reference
• A dynein light intermediate chain, D1bLIC, is required for retrograde intraflagellar transport.

  2004influential reference
• Molecular Map of the Chlamydomonas reinhardtii Nuclear Genome

  2003cited by this paper
• Intraflagellar transport

  2002cited by this paper
• Tetrahymena thermophila contains a conventional γ-tubulin that is differentially required for the maintenance of different microtubule-organizing centers

  2002cited by this paper
• Analysis of cell vibration for assessing axonemal motility in Chlamydomonas.

  2000cited by this paper
• Distribution of polyglutamylated tubulin in the flagellar apparatus of green flagellates.

  2000cited by this paper
• The DHC1b (DHC2) Isoform of Cytoplasmic Dynein Is Required for Flagellar Assembly

  1999cited by this paper
• Chlamydomonas Kinesin-II–dependent Intraflagellar Transport (IFT): IFT Particles Contain Proteins Required for Ciliary Assembly in Caenorhabditis elegans Sensory Neurons

  1998cited by this paper
• The role of preassembled cytoplasmic complexes in assembly of flagellar dynein subunits.

  1998cited by this paper
• Chlamydomonas Inner-Arm Dynein Mutant, ida5, Has a Mutation in an Actin-encoding Gene

  1997cited by this paper
• Immunofluorescence microscopy of cilia and flagella.

  1995cited by this paper
• The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane

  1995cited by this paper
• Molecular analysis of the gamma heavy chain of Chlamydomonas flagellar outer-arm dynein.

  1994cited by this paper
• The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein

  1994influential reference
• Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer-arm-missing axonemes.

  1992cited by this paper
• Mutations at twelve independent loci result in absence of outer dynein arms in Chylamydomonas reinhardtii

  1988cited by this paper
• Redistribution and shedding of flagellar membrane glycoproteins visualized using an anti-carbohydrate monoclonal antibody and concanavalin A

  1986cited by this paper
• A motile Chlamydomonas flagellar mutant that lacks outer dynein arms

  1985cited by this paper
• A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation

  1985cited by this paper
• A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.

  1984cited by this paper
• Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

  1981cited by this paper
• Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

  1979cited by this paper
• Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components

  1978cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970influential reference

• Tubulin glycylation controls ciliary motility through modulation of outer-arm dyneins

  2024cites this paper
• Structural basis for α-tubulin-specific and modification state-dependent glutamylation

  2024cites this paper
• TTLL12 is required for primary ciliary axoneme formation in polarized epithelial cells

  2023cites this paper
• TTLL12 is required for primary ciliary axoneme formation in polarized epithelial cells

  2023cites this paper
• Supplemental Materials

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  2020cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2017cites this paper
• Posttranslational Modifications of Tubulin and Cilia.

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  2017cites this paper
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  2016cites this paper
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  2016cites this paper
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