Engineering and characterization of small-molecule transporters for cell factories.

Membrane transporters play crucial roles in metabolite exchange, cellular communication, and metabolic homeostasis and are attractive targets in metabolic engineering for the development of microbial cell factories. While transporter engineering has proven effective in enhancing nutrient uptake, improving product secretion, and optimizing metabolic flux, its broader application is limited by incomplete knowledge of membrane transport systems and the sometimes high promiscuity of transporters, often resulting in unpredictable outcomes. This review provides an overview of recent progress in transporter engineering and characterization methods, highlighting their potential to enhance the production of bio-based chemicals.

• Publication year

  2025

• Venue

  Trends in Biotechnology

• Publication date

  2025-12-01

• Fields of study

  Medicine, Chemistry, Engineering, Environmental Science

• Identifiers
  DOI 10.1016/j.tibtech.2025.11.019PMID 41419347
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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