Identification of Candidate Genes Controlling Resistance to Verticillium dahliae Using CCRI70 Recombinant Inbred Line Population in Gossypium hirsutum.

Verticillium wilt (VW) severely limits the cotton yield and fiber quality. Marker-assisted selection is an efficient strategy for breeding resistant varieties. In this study, a high-density genetic map was constructed by using an F8:9 recombinant inbred line (RIL) population derived from CCRI70. Phenotypic data on disease incidence (DINC) and disease index (DI) were collected across six environments. A total of 59 QTLs for DINC and 60 QTLs for DI were identified, with three and six stable across multiple environments, respectively. These QTLs formed 18 clusters across 13 chromosomes, showing consistent additive effects. Transcriptome analysis revealed eight differentially expressed candidate genes within stable QTL regions. Among them, GH_D05G1495, GH_A09G1013, and GH_D05G1683 were further validated by virus-induced gene silencing as key genes conferring V. dahliae resistance in cotton. This study provides valuable genetic resources for improving Verticillium wilt resistance in cotton breeding.

• Publication year

  2025

• Venue

  Journal of Agricultural and Food Chemistry

• Publication date

  2025-12-30

• Fields of study

  Agricultural and Food Sciences, Medicine

• Identifiers
  DOI 10.1021/acs.jafc.5c10468PMID 41469895
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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