Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution

Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.

• Publication year

  2010

• Venue

  Nature Methods

• Publication date

  2010-01-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/nmeth.1414PMID 20062050PMCID 2860480
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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