Gene Expression of MicroRNA-205, FGF2 and CARMA3 in Colorectal Cancer in Iraqi Patients.

BACKGROUND Colorectal cancer (CRC) is a growing global health concern. This study focuses on evaluating the gene expression of FGF2, CARMA3, and MicroRNA205 (miR205) as potential blood-based biomarkers for early CRC diagnosis by distinguishing patients from healthy controls. MATERIALS AND METHODS Blood samples from 80 colorectal cancer (CRC) patients and 40 healthy controls were analyzed using qRT- PCR to measure target gene expression. ROC curve analysis was performed to evaluate diagnostic accuracy, including sensitivity, specificity, and Area Under the Curve (AUC) values. RESULTS MicroRNA-205 revealed downregulation in CRC patients, with an AUC of 0.7, sensitivity of 91%, and specificity of 58%. AUC values for Fibroblast Growth Factor 2 (FGF2) and Caspase Recruitment Domain Family Member 3 (CARMA3) were 0.74 and 0.77, respectively, indicating differential expression. All markers displayed modest specificity, but CARMA3 showed the highest diagnostic accuracy with 95% sensitivity. Gene expression fold change for miR-205 showed downregulation 0.3, FGF2 also showed downregulation 0.4 and CARMA3 showed slight upregulation 1.2. CONCLUSION The results suggest that miR-205, FGF2, and CARMA3 may serve as potential biomarkers for the identification of colorectal cancer (CRC), particularly when used in multi-marker panels to improve diagnostic accuracy. Their clinical utility should be confirmed through additional validation in larger cohorts.

• Publication year

  2026

• Venue

  Asian Pacific Journal of Cancer Prevention

• Publication date

  2026-02-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.31557/APJCP.2026.27.2.509PMID 41660908
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Expression of Protamine-1 Gene and Association with Interleukin-12 gene in Infertile Men: A Case-Control Study in Iraqi Patients

  2023cited by this paper
• pyBrick-DNA: A Python-Based Environment for Automated Genetic Component Assembly

  2023cited by this paper
• microRNA-205 in prostate cancer: Overview to clinical translation.

  2022cited by this paper
• A review on breast cancer in Iraq and future therapies insights

  2022cited by this paper
• Assessment of Interleukin-8 in Bronchial Asthma in Iraq.

  2021cited by this paper
• Trends in Breast Cancer Incidence in Iraq During the Period 2000-2019

  2021influential reference
• CARMA3 Promotes Colorectal Cancer Cell Motility and Cancer Stemness via YAP-Mediated NF-κB Activation

  2021cited by this paper
• Discovering cooperative biomarkers for heterogeneous complex disease diagnoses

  2019cited by this paper
• FGF Signalling in the Self-Renewal of Colon Cancer Organoids

  2019cited by this paper
• HiLight-PTM: an online application to aid matching peptide pairs with isotopically labelled PTMs

  2019cited by this paper
• Targeting the Oncogenic FGF-FGFR Axis in Gastric Carcinogenesis

  2019cited by this paper
• NF-kappaB-inducing kinase in cancer.

  2019cited by this paper
• Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries

  2018cited by this paper
• Bevacizumab for malignant gliomas: current indications, mechanisms of action and resistance, and markers of response

  2017cited by this paper
• Systematic identification of human housekeeping genes possibly useful as references in gene expression studies

  2017cited by this paper
• Noncanonical NF-κB Signaling in Health and Disease.

  2016cited by this paper
• Prognostic role of microRNA-205 in multiple human malignant neoplasms: a meta-analysis of 17 studies

  2015cited by this paper
• The important roles of miR-205 in normal physiology, cancers and as a potential therapeutic target.

  2014cited by this paper
• Diagnostic and prognostic value of miR-205 in colorectal cancer.

  2014cited by this paper
• miRNA as markers for the diagnostic screening of colon cancer

  2014cited by this paper
• Receiver Operating Characteristic (ROC) Curve Analysis for Medical Diagnostic Test Evaluation.

  2013influential reference
• MicroRNA dysregulation in cancer: diagnostics, monitoring and therapeutics. A comprehensive review

  2012influential reference
• Fibroblast growth factor signalling: from development to cancer

  2010cited by this paper
• Antigen receptor signaling to NF-kappaB via CARMA1, BCL10, and MALT1.

  2010cited by this paper
• Body-mass index and incidence of cancer: a systematic review and meta-analysis of prospective observational studies.

  2008cited by this paper
• Inflammation and cancer: Role of nuclear factor‐kappaB activation

  2008cited by this paper
• The global health burden of infection‐associated cancers in the year 2002

  2006cited by this paper
• Nuclear factor-kappaB in cancer development and progression.

  2006cited by this paper
• MicroRNA signatures in human cancers

  2006cited by this paper
• GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues.

  2005cited by this paper
• Intensified Cloning Efforts Have Revealed Numer

  2004cited by this paper
• Faculty Opinions recommendation of IFNgamma and lymphocytes prevent primary tumour development and shape tumour immunogenicity.

  2001cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Primer3 on the WWW for general users and for biologist programmers.

  2000cited by this paper
• Fibroblast growth factors, their receptors and signaling.

  2000influential reference
• Molecular classification of cancer: class discovery and class prediction by gene expression monitoring.

  1999cited by this paper
• A genetic model for colorectal tumorigenesis.

  1990cited by this paper

• No citing papers are available for this paper.