SCIENCE@home

Endogenous Metabolic Incorporation of Inhaled Soot Particles Revealed by Bottom-up 13C Labeling.

Weican Zhang,Zhanyu Su,Shaowei Yan,Peng Zhang,Yue Lin,Hongyan He,Qian S Liu,Guibin Jiang

Published 2026 in Environmental Science and Technology

ABSTRACT

Inhalation of soot particles generated from both wildfires and incomplete combustion of fossil fuels poses a severe threat to global human health and is associated with many diseases. However, the metabolic transformation of soot particles, a key link between soot exposure and health outcomes, remains poorly understood. Here, we develop a novel bottom-up method to rapidly synthesize 13C-labeled soot, enabling in vivo tracking of soot metabolism by mass spectrometry. In vitro metabolic experiments utilizing human liver postmitochondrial liver supernatant (S9) fractions show the potential involvement of soot in human endogenous metabolism. Using animal exposure models combined with nontargeted metabolic flux analysis, we further demonstrate that soot particles can be utilized as a carbon source by the organism and directly integrated into biological metabolic processes, subsequently affecting metabolites and potential pathways associated with diseases. Our findings suggest that no clear-cut boundary exists between exogenous inert soot particles and endogenous metabolites, offering novel insights into the "black box" of internal exposure of air pollution and their associated pathogenic mechanisms.

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-85 of 85 references · Page 1 of 1

CITED BY

  • No citing papers are available for this paper.

Showing 0-0 of 0 citing papers · Page 1 of 1