A high-yielding, generic fed-batch cell culture process for production of recombinant antibodies.

No abstract is available for this paper.

• Publication year

  2000

• Venue

  Biotechnology and Bioengineering

• Publication date

  2000-03-05

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/(SICI)1097-0290(20000305)67:5<585::AID-BIT9>3.0.CO;2-HPMID 10649233
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1997cited by this paper
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  1997cited by this paper
• High density fed-batch cultures for hybridoma cells performed with the aid of a kinetic model

  1996cited by this paper
• The importance of ammonia in mammalian cell culture.

  1996cited by this paper
• High viable cell concentration fed‐batch cultures of hybridoma cells through on‐line nutrient feeding

  1995cited by this paper
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  1995cited by this paper
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  1995cited by this paper
• Monoclonal Antibody Process Development Using Medium Concentrates

  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1992cited by this paper
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  1991cited by this paper
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  1991cited by this paper
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  1990cited by this paper
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  1989cited by this paper
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  1988cited by this paper
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  1986cited by this paper
• [1] Preparation of monoclonal antibodies: Strategies and procedures

  1981cited by this paper
• A better cell line for making hybridomas secreting specific antibodies

  1978cited by this paper
• The effects of adapting human diploid cells to grow in glutamic acid media on cell morphology, growth and metabolism.

  1973cited by this paper

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