An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide.

No abstract is available for this paper.

• Publication year

  1989

• Venue

  Nucleic Acids Research

• Publication date

  1989-02-11

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1093/NAR/17.3.1266
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• No references are available for this paper.

• Modular universal rbcL primers for (meta)barcoding: a systematic case study clarifying the identity of the marine macroalga Corallina armata (Rhodophyta, Corallinales)

  2026cites this paper
• Fluorescent HIV-1 integrases for a suite of new user-friendly stability, nucleic acid binding and strand transfer activity assays.

  2025cites this paper
• Conception and Optimization of Extraction-Free Loop-Mediated Isothermal Amplification Detection of Dry Rot Fungus Serpula lacrymans

  2024cites this paper
• Protein folding, cellular stress and cancer.

  2024cites this paper
• Reducing COVID-19 diagnostic errors with dNTPαSe supplementation.

  2023cites this paper
• Near Quantitative Ligation Results in Resistance of DNA Origami Against Nuclease and Cell Lysate

  2023cites this paper
• Efecto de NUAK1 en el splicing de G6PD dependiente de hnRNPK en células de cáncer colorrectal.

  2023cites this paper
• Dimethyl sulfoxide (DMSO) is a stabilizing co-solvent for G-quadruplex DNA.

  2022cites this paper
• PCR enhancers: Types, mechanisms, and applications in long-range PCR.

  2022cites this paper
• Combining Direct PCR Technology and Capillary Electrophoresis for an Easy-to-Operate and Highly Sensitive Infectious Disease Detection System for Shrimp

  2022cites this paper
• Insight into the nanomaterials enhancement mechanism of nucleic acid amplification reactions

  2021cites this paper
• DNA detection by SERS: hybridisation parameters and the potential for asymmetric PCR.

  2020cites this paper
• Polymerase Chain Reaction (PCR) Amplification of GC-Rich Templates.

  2019cites this paper
• Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes

  2018cites this paper
• Investigations of LIMD1 in miRNA-mediated gene silencing and cancers

  2018cites this paper
• METHOD OF OPTIMIZING AMPLIFICATION IN PCR BACKGROUND OF THE PRESENT INVENTION

  2017cites this paper
• ASSOCIATED WITH STRUCTURAL ALTERATIONS OF THE EGF RECEPTOR GENE IN HUMAN TUMIORS

  2017cites this paper
• A predicted Hairpin Corresponds to Resistance of Chimeric 1F5 Heavy gene to DNA Polymerase

  2017cites this paper
• Catalytic Activities of Ribozymes and DNAzymes in Water and Mixed Aqueous Media

  2017cites this paper
• Improved PCR Amplification of Broad Spectrum GC DNA Templates

  2016cites this paper
• The structural stability and catalytic activity of DNA and RNA oligonucleotides in the presence of organic solvents

  2016cites this paper
• Guidelines for DNA taxonomy, with a focus on the meiofauna

  2015cites this paper
• Diversity of Baculoviruses isolated from cutworms ( Agrotis spp.)

  2015cites this paper
• The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases

  2015cites this paper
• Attempted Construction of Recombinant Vectors Designed to Study the Solubility of Overexpressed Proteinase Inhibitor 2 when Co-expressed with Thioredoxin

  2015cites this paper
• Identifikation und Charakterisierung von Perlucin Spleißvarianten der marinen Abalone Haliotis laevigata.

  2014cites this paper
• Reticulate evolution in North American black-fruited hawthorns ( Crataegus section Douglasia ; Rosaceae): evidence from nuclear ITS2 and plastid sequences

  2014cites this paper
• Identification of a novel mutation in the CLN6 gene (CLN6) in South Hampshire sheep affected with Neuronal Ceroid Lipofuscinosis

  2014influential citation
• Reticulate evolution in North American black-fruited hawthorns (Crataegus section Douglasia; Rosaceae): evidence from nuclear ITS2 and plastid sequences.

  2014cites this paper
• A method for the genetically encoded incorporation of FRET pairs into proteins

  2014cites this paper
• Quantum dots trigger hot-start effects for pfu-based polymerase chain reaction

  2014cites this paper
• Primer Based Approach for PCR Amplification of High GC Content Gene: Mycobacterium Gene as a Model

  2014cites this paper
• Analysis of mRNA Expression by Real-time PCR

  2013influential citation
• Similar Levels of mRNA from the W 1282 Xand the AF 508 Cystic Fibrosis Alleles , in Nasal Epithelial Cells

  2013cites this paper
• Angiotensin-converting enzyme insertion/deletion polymorphism genotyping error: the cause and a possible solution to the problem

  2013cites this paper
• Endothelin-1 mRNAIs Widely Expressed in Porcine and HumanTissues

  2013cites this paper
• DIMETHYL SULFOXIDE IMPROVES SENSITIVITY AND SPECIFICITY OF RT-PCR AND QRT-PCR AMPLIFICATION OF LOW-EXPRESSED TRANSGENES

  2012cites this paper
• Detection of Human Rotavirus in Southern Ontario Source Waters

  2012cites this paper
• Quantum dots induce hot-start effects for Taq-based polymerase chain reaction

  2012cites this paper
• Gene Assembly from Chip‐Synthesized Oligonucleotides

  2012cites this paper
• A predicted hairpin cluster correlates with barriers to PCR, sequencing and possibly BAC recombineering

  2011cites this paper
• Developing Somatic Hypermutation as a Protein Engineering Tool to Study Angiopoietin Binding

  2011cites this paper
• Molecular systematics of the Lomandra Labill. Complex (Asparagales: Laxmanniaceae).

  2010cites this paper
• DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis

  2010cites this paper
• Optimierung der DNA-Analyse für degradierte biologische Spuren

  2010cites this paper
• Entwicklung eines retroviralen shRNA-basierten Vektorsystems zur Untersuchung BCR-ABL-abhängiger Signalkaskaden in vitro und in vivo

  2010cites this paper
• The its Region of Nuclear Ribosomal DNA: A Valuable Source of Evidence on Angiosperm Phylogeny

  2010cites this paper
• Hybrid Origin and Parentage of Dendrochilum acuiferum (Orchidaceae) Inferred in a Phylogenetic Context Using Nuclear and Plastid DNA Sequence Data

  2009cites this paper
• Designing of a Biochemical Kit Compatible with low-cost Real-Time PCR Instrument for Global Clinic 2008 / 2009

  2009cites this paper
• Molecular systematics of Gagea and Lloydia (Liliaceae; Liliales): implications of analyses of nuclear ribosomal and plastid DNA sequences for infrageneric classification.

  2009cites this paper
• Annals of Internal Medicine Transmission of Hepatitis A to Patients with Hemophilia by Factor VIII Concentrates Treated with Organic Solvent and Detergent To Inactivate Viruses

  2009cites this paper
• The Product of the CYP 11 B 2 Gene Is Required for Aldosterone Biosynthesis in the Human Adrenal Cortex

  2008cites this paper
• Human papillomavirus genotypes and HPV16 variants in penile carcinoma

  2008influential citation
• Human papillomavirus (HPV) genotypes and HPV16 variants in human immunodeficiency virus-positive Italian women.

  2008cites this paper
• A pilot study on the distribution of human papillomavirus genotypes and HPV-16 variants in cervical neoplastic lesions from Ecuadorian women

  2008cites this paper
• Functional Characterization by Genetic Complementation of aroB-Encoded Dehydroquinate Synthase from Mycobacterium tuberculosis H37Rv and Its Heterologous Expression and Purification

  2007influential citation
• Sea ice occurrence predicts genetic isolation in the Arctic fox

  2007influential citation
• New Buffers to Improve the Quantitative Real-Time Polymerase Chain Reaction

  2007cites this paper
• Phylogenetics of Chloridoideae (Gramineae): a Preliminary Study Based on Nuclear Ribosomal Internal Transcribed Spacer and Chloroplast trnL–F Sequences

  2007cites this paper
• Prevalence of alpha‐papillomavirus genotypes in cervical squamous intraepithelial lesions and invasive cervical carcinoma in the Italian population

  2006cites this paper
• A heteroduplex-preferential Tm depressor for the specificity-enhanced DNA polymerase chain reactions.

  2005cites this paper
• Molecular phylogenetic evidence for the monophyly of Fritillaria and Lilium (Liliaceae; Liliales) and the infrageneric classification of Fritillaria.

  2005cites this paper
• SEQUENCE ANALYSIS OF AMPLIFIED t ( 14 ; 18 ) LYMPHOMAS CHROMOSOMAL BREAKPOINTS

  2005cites this paper
• Fungi at the edge of life: cryptendolithic black fungi from Antarctic desert

  2005cites this paper
• Interindividual variability and parent of origin DNA methylation differences at specific human Alu elements.

  2005cites this paper
• Cloning, sequencing, and expression inEscherichia coli of the coat protein gene of a new potyvirus infecting South AfricanPassiflora

  2005cites this paper
• Investigation of the relationship between osteoporosis and the collagenase gene by means of polymorphism of the 5′upstream region of this gene

  2004cites this paper
• Ten HLA-DR4 alleles defined by sequence polymorphisms within the DRB1 first domain

  2004cites this paper
• Identification des conditions optimales d'utilisation de l'ADN polymérase Vent exo- dans la technologie LMPCR

  2004cites this paper
• Determination of dopamine receptor (D2) -141C Ins/Del polymorphism in whole- blood and buccal swabs by PCR-RFLP genotyping using GC-Rich ® solution

  2004cites this paper
• Analysis of human papillomavirus type‐16 variants in Italian women with cervical intraepithelial neoplasia and cervical cancer

  2004cites this paper
• A biolistic approach towards producing transgenic Pinus patula embryonal suspensor masses

  2004cites this paper
• Nucleic acid sequences of the H-2Ksand H-2Ksm1genes

  2004cites this paper
• Unique sequences for two HLA-DRB1 genes expressed on distinct DRw6 haplotypes

  2004cites this paper
• Population screening of lactate dehydrogenase deficiencies in Fukuoka Prefecture in Japan and molecular characterization of three independent mutations in the lactate dehydrogenase-B(H) gene

  2004cites this paper
• Amplification efficiency of thermostable DNA polymerases.

  2003influential citation
• Ribosomal ITS sequences and plant phylogenetic inference.

  2003cites this paper
• Determination of HCV genotype by direct sequence analysis of quantitative PCR products

  2003cites this paper
• Mutation detection for genotype/phenotype correlation studies in autosomal dominant polycystic kidney disease

  2002cites this paper
• Phylogenetic relationships within Plantago (Plantaginaceae): evidence from nuclear ribosomal ITS and plastid trnL-F sequence data

  2002cites this paper
• A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation

  2001cites this paper
• The enhancement of PCR amplification by low molecular weight amides.

  2001cites this paper
• Can sucrose affect polymerase chain reaction product formation?

  2001influential citation
• Genetic heterogeneity of SAT-1 type foot-and-mouth disease viruses in southern Africa

  2001cites this paper
• Pilot studies on the p53 gene in nipple aspirate fluid from patients with breast cancer

  2000cites this paper
• Clinical Evaluation of the Automated COBAS AMPLICOR HCV MONITOR Test Version 2.0 for Quantifying Serum Hepatitis C Virus RNA and Comparison to the Quantiplex HCV Version 2.0 Test

  2000cites this paper
• Phylogenetic relationships of the durians (Bombacaceae-Durioneae or /Malvaceae/Helicteroideae/Durioneae) based on chloroplast and nuclear ribosomal DNA sequences

  2000cites this paper
• Characterization and analysis of genes involved in colorectal and endometrial cancer

  2000cites this paper
• Identification and functional analysis of sequence rearrangements in the long control region of human papillomavirus type 16 Af-1 variants isolated from Ugandan penile carcinomas.

  2000cites this paper
• High hopanoid/total lipids ratio in Frankia mycelia is not related to the nitrogen status.

  2000cites this paper
• Internal transcribed spacer sequence phylogeny of Crambe L. (Brassicaceae): molecular data reveal two Old World disjunctions.

  1999cites this paper
• Hemophilia B mutational analysis.

  1999cites this paper
• Detection of a normally rare transcript in propionic acidemia patients with mRNA destabilizing mutations in the PCCA gene.

  1999cites this paper
• Characterisation of a gene trap integration marking hepatic specification

  1999cites this paper
• Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis

  1999cites this paper
• Molecular analysis of methylmalonyl-CoA mutase deficiency: identification of three missense mutations in mut0 patients*

  1999cites this paper
• Analysis of Frankia evolutionary radiation using glnII sequences.

  1999cites this paper
• p53 mutations as a marker of malignancy in bladder washing samples from patients with bladder cancer

  1999cites this paper
• Mutation rates in humans. I. Overall and sex-specific rates obtained from a population study of hemophilia B.

  1999cites this paper
• Deleted HTLV provirus in peripheral blood cells of a patient with T‐cell prolymphocytic leukaemia

  1998cites this paper