Additive effects of bovine serum albumin, dithiothreitol and glycerolon PCR

No abstract is available for this paper.

• Publication year

  1998

• Venue

  Biochemistry and molecular biology international

• Publication date

  1998-01-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1080/15216549800201172PMID 9503159
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Substances interfering with direct detection of Mycobacterium tuberculosis in clinical specimens by PCR: effects of bovine serum albumin

  1996cited by this paper
• Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

  1996cited by this paper
• Simultaneous detection of microorganisms in soil suspension based on PCR amplification of bacterial 16S rRNA fragments.

  1996cited by this paper
• Sex determination by polymerase chain reaction on mummies discovered at Taklamakan desert in 1912.

  1995cited by this paper
• Improved sizing of fragile X CCG repeats by nested polymerase chain reaction.

  1994cited by this paper
• Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification.

  1994cited by this paper
• Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases.

  1994cited by this paper
• PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.

  1994cited by this paper
• Effective amplification of long targets from cloned inserts and human genomic DNA.

  1994cited by this paper
• The effect of polyols on the stability of duplex DNA.

  1993cited by this paper
• Use of glycerol for enhanced efficiency and specificity of PCR amplification.

  1993cited by this paper
• Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR.

  1993cited by this paper
• Activity and dimerization of human immunodeficiency virus protease as a function of solvent composition and enzyme concentration.

  1992cited by this paper
• Branch capture reactions: displacers derived from asymmetric PCR.

  1991cited by this paper
• Organic solvents as facilitators of polymerase chain reaction.

  1991cited by this paper
• Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.

  1989cited by this paper
• Amplification and analysis of DNA sequences in single human sperm and diploid cells

  1988cited by this paper
• DNA typing from single hairs

  1988cited by this paper
• Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction

  1988cited by this paper
• An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. Application to hemophilia A.

  1987cited by this paper
• Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides.

  1987cited by this paper
• Factors of activation and stabilization of DNA-methylases from Shigella sonnei 47 and Mycobacterium smegmatis butyricum cells.

  1987cited by this paper
• Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.

  1986cited by this paper
• Thermodynamic and kinetic examination of protein stabilization by glycerol.

  1981cited by this paper
• Increased thermal stability of proteins in the presence of sugars and polyols.

  1979cited by this paper
• A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

  1979cited by this paper
• DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS.

  1964cited by this paper

• Development of a multiplex ddPCR assay for simultaneous absolute quantification of bacterial, fungal, and human DNA.

  2026cites this paper
• Observation Bias in Metabarcoding

  2025cites this paper
• Development of multiplex PCR for detection of foodborne pathogens in fresh produce.

  2025cites this paper
• Inhibition of polymerase chain reaction by hydrogel monomers

  2025cites this paper
• The improvement of PCR amplification pattern by PCR enhancers and formulation novel PCR enhancer

  2024influential citation
• Assessment of Common Factors Associated with Droplet Digital PCR (ddPCR) Quantification of Paratrichodorus allius in Soil

  2024cites this paper
• Widespread establishment of adventive populations of Leptopilina japonica (Hymenoptera, Figitidae) in North America and development of a multiplex PCR assay to identify key parasitoids of Drosophila suzukii (Diptera, Drosophilidae)

  2024cites this paper
• Mid-Infrared Photons Enhance Polymerase Chain Reaction Efficiency by Strong Coupling with Vibrational DNA Molecules

  2023cites this paper
• PCR enhancers: Types, mechanisms, and applications in long-range PCR.

  2022cites this paper
• A molecular phylogeny of Thuridilla Bergh, 1872 sea slugs (Gastropoda, Sacoglossa) reveals a case of flamboyant and cryptic radiation in the marine realm

  2021cites this paper
• Microbiota and Biofertilizers: A Sustainable Continuum for Plant and Soil Health

  2021cites this paper
• PCR Optimization for Beginners: A Step by Step Guide

  2021cites this paper
• A chemical-enhanced system for CRISPR-Based nucleic acid detection

  2021cites this paper
• Long-read Sequencing of Eukaryotic rDNA Operon from the Singapore Marine Straits

  2020cites this paper
• Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis

  2020cites this paper
• A blueprint for academic labs to produce SARS-CoV-2 RT-qPCR test kits

  2020cites this paper
• Root-Associated Ectomycorrhizal Mycobionts as Forest Biofertilizers: Standardized Molecular Methods for Characterization of Ectomycorrhizal Wood Wide Web

  2020cites this paper
• DMSO and betaine significantly enhance the PCR amplification of ITS2 DNA barcodes from plants.

  2020cites this paper
• Phylogenetic Techniques in Geomicrobiology

  2019cites this paper
• Pullulan reduces the non-specific amplification of loop-mediated isothermal amplification (LAMP)

  2019cites this paper
• Direct multiplex allele-specific PCR amplification for ABO genotyping from whole blood, hair root and buccal cell

  2019cites this paper
• Transition Metal Dichalcogenide Nanosheets for Visual Monitoring PCR Rivaling a Real-Time PCR Instrument.

  2018cites this paper
• Circulating DNA for the in vivo detection and monitoring of lung cancer

  2018cites this paper
• Development of gold nanoparticles biosensor for ultrasensitive diagnosis of foot and mouth disease virus

  2018cites this paper
• A universal method for direct PCR amplification of plant tissues

  2017cites this paper
• Application of gold nanoparticle-assisted PCR for equine herpesvirus 1 diagnosis in field samples

  2017cites this paper
• METHOD OF OPTIMIZING AMPLIFICATION IN PCR BACKGROUND OF THE PRESENT INVENTION

  2017cites this paper
• One‐Pot Synthesis of Multiple Protein‐Encapsulated DNA Flowers and Their Application in Intracellular Protein Delivery

  2017cites this paper
• Scale samples from Temminck’s ground pangolin (Smutsia temminckii): a non-invasive source of DNA

  2017cites this paper
• Modyfikacje polimeraz DNA jako rozwiązania problemów w reakcjach PCR

  2017cites this paper
• PCR Technologies for Point of Care Testing: Progress and Perspectives.

  2017cites this paper
• Effect of surface functionalisation on the interaction of iron oxide nanoparticles with polymerase chain reaction.

  2017cites this paper
• Comparison of the Lipophosphoglycan 3 Gene of the Lizard and Mammalian Leishmania: A Homology Modeling

  2016cites this paper
• Modified DNA polymerases for PCR troubleshooting

  2016cites this paper
• Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time

  2016cites this paper
• Comprehensive evaluation of molecular enhancers of the isothermal exponential amplification reaction

  2016cites this paper
• Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification

  2016cites this paper
• A multilocus molecular phylogeny of Fasciolariidae (Neogastropoda: Buccinoidea).

  2016cites this paper
• Life in the Anoxic Sub-Seafloor Environment: Linking Microbial Metabolism and Mega Reserves of Methane Hydrate.

  2016cites this paper
• Basic Rules in Nucleic Acid-Mediated Amplification and Hybridization Methods in Food Safety Detection: A Review

  2016cites this paper
• Fidelity quantification of mercury(II) ion via circumventing biothiols-induced sequestration in enzymatic amplification system

  2016cites this paper
• The effect of gold nanoparticles on the diagnostic polymerase chain reaction technique for equine herpes virus 1 (EHV-1)

  2016cites this paper
• Gold nanoparticle-assisted polymerase chain reaction: effects of surface ligands, nanoparticle shape and material

  2016cites this paper
• Development of a real-time PCR assay (SYBR Green I) for rapid identification and quantification of scyphomedusae Aurelia sp.1 planulae

  2015cites this paper
• Cellulases and xylanase production by 'Aspergillus fumigatus' SK1 through solid state fermentation for ethanol fermentation

  2015cites this paper
• Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

  2015cites this paper
• Development of a Nucleic Acid Extraction Procedure for Simultaneous Recovery of DNA and RNA from Diverse Microbes in Water

  2015cites this paper
• Properties of targeted preamplification in DNA and cDNA quantification

  2015cites this paper
• Utility of amplification enhancers in low copy number DNA analysis

  2014cites this paper
• Development and evaluation of a novel combinatorial selective enrichment and multiplex PCR technique for molecular detection of major virulence‐associated genes of enterotoxigenic Staphylococcus aureus in food samples

  2014cites this paper
• Population genetic structure of the ground pangolin based on mitochondrial genomes

  2014cites this paper
• Bovine thrombin enhances the efficiency and specificity of polymerase chain reaction.

  2014cites this paper
• Amplification of GC-rich DNA for High-Throughput Family-Based Genetic Studies

  2013cites this paper
• Enhancement of PCR Amplification of Moderate GC-Containing and Highly GC-Rich DNA Sequences

  2013cites this paper
• Application of Mitochondrial DNA Analysis in Contemporary and Historical Samples

  2013cites this paper
• Effect of surface charge of PDDA-protected gold nanoparticles on the specificity and efficiency of DNA polymerase chain reaction.

  2013cites this paper
• Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 950 Application of Mitochondrial DNA Analysis in Contemporary and Historical Samples

  2013cites this paper
• Optimization of DNA isolation process and enhancement of RAPD PCR for low quality genomic DNA of Terminalia arjuna

  2013cites this paper
• Mechanism studies on nanoPCR and applications of gold nanoparticles in genetic analysis.

  2013cites this paper
• Development and evaluation of molecular-based assays for detecting Salmonella serovars in various food commodities

  2013cites this paper
• Development of an isothermal nucleic acid amplification protocol for high-throughput monitoring of Plum pox virus infection in stone fruit production

  2013cites this paper
• Rapid direct PCR for forensic genotyping in under 25 min

  2013cites this paper
• The development of direct ultra-fast PCR for forensic genotyping using short channel microfluidic systems with enhanced sieving matrices

  2012cites this paper
• Identification of algal strains by PCR amplification and evaluation of their fatty acid profiles for biodiesel production

  2012cites this paper
• Elucidation of colonization time and prevalence of thermophilic Campylobacter species during turkey rearing using multiplex polymerase chain reaction.

  2012cites this paper
• Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART

  2012cites this paper
• Development of species-specific polymerase chain reaction (PCR), real-time PCR and loop mediated isothermal amplifications (LAMP) assays for detection of Anaplamsa marginale strains in South Africa

  2012cites this paper
• Sequencing and detection of a new strain of grapevine leafroll-associated virus 3 in South Africa

  2012cites this paper
• Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI

  2012cites this paper
• DIMETHYL SULFOXIDE IMPROVES SENSITIVITY AND SPECIFICITY OF RT-PCR AND QRT-PCR AMPLIFICATION OF LOW-EXPRESSED TRANSGENES

  2012influential citation
• Molecular identification of Penicillium marneffei using rolling circle amplification

  2011cites this paper
• Taxonomy, biodiversity, and ecology of Apusozoa (Protozoa)

  2011cites this paper
• Regulation of PCR efficiency with magnetic nanoparticles in a rotating magnetic field

  2011cites this paper
• Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA)

  2011cites this paper
• Nanomaterials-based Polymerase Chain Reactions for DNA Detection

  2011cites this paper
• Rapid identification and characterization of Penicillium marneffei using multiplex ligation-dependent probe amplification (MLPA) in paraffin-embedded tissue samples.

  2011cites this paper
• 用于核酸的合成和/或检测的组合物、试剂盒及方法

  2011cites this paper
• Optimierung der DNA-Analyse für degradierte biologische Spuren

  2010cites this paper
• Búsqueda de genes codificadores de lipasas en una biblioteca metagenómica de suelo de bosque alto andino mediante PCR

  2010cites this paper
• Optimization of PCR in application of hot start Taq DNA polymerase for detection of Erwinia amylovora with primers FER1-F and FER1-R1

  2010cites this paper
• An ALE-Index Based Algorithm Facilitated Prediction of Success for Polymerase Chain Reactions

  2009cites this paper
• Qualitative analysis of Adenomatous Polyposis Coli promoter: Hypermethylation, engagement and effects on survival of patients with esophageal cancer in a high risk region of the world, a potential molecular marker

  2009cites this paper
• Identificación y clonación de nuevas celulasas para la producción de bioetanol de segunda generación

  2009cites this paper
• The Human Vaginal Bacterial Biota and Bacterial Vaginosis

  2009cites this paper
• An Effective Combined-Enhancer for the PCR Amplification of GC-Rich Longer DNA Sequences

  2009cites this paper
• IDENTIFICACIÓN Y CLONACIÓN DE NUEVAS CELULASAS PARA LA PRODUCCIÓN DE BIOETANOL DE SEGUNDA GENERACIÓN

  2009cites this paper
• Development and Optimization of Quantitative PCR Assays to Aid Ostrea lurida Carpenter 1864 Restoration Efforts

  2009cites this paper
• Assessing the Mycorrhizal Diversity of Soils and Identification of Fungus Fruiting Bodies and Axenic Cultures

  2009influential citation
• SIZE AND SURFACE EFFECT OF GOLD NANOPARTICLES (AuNPs) IN NANOGOLD-ASSISTED PCR

  2008cites this paper
• Phylogeography of three Southern African endemic elephant-shrews and a supermatrix approach to the Macroscelidea

  2008cites this paper
• Verfahren und testkit zum schnellen nachweis spezifischer nukleinsäuresequenzen, insbesondere zum nachweis von mutationen oder snp's

  2008cites this paper
• Detection and Discrimination of Pratylenchus neglectus and P. thornei in DNA Extracts from Soil.

  2008cites this paper
• Nanogold-assisted multi-round polymerase chain reaction (PCR).

  2007cites this paper
• Detection of Erwinia amylovora by novel chromosomal polymerase chain reaction primers

  2007cites this paper
• An efficient and economic enhancer mix for PCR.

  2006influential citation
• The inhibitory effect of pentobarbitone on reverse transcription-PCR.

  2005cites this paper
• Strategies to Generate PCR-Compatible Samples

  2004cites this paper
• Pre-PCR processing

  2004cites this paper
• Pre-PCR processing of samples.

  2003cites this paper
• Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark.

  2003cites this paper