Cloning and Characterization of a Calcium-binding, Histamine-releasing Protein from Schistosoma mansoni *

A homologue of the mammalian translationally controlled tumor protein (TCTP) was cloned from the human parasiteSchistosoma mansoni (SmTCTP). Sequence analysis showed that SmTCTP differed from other reported TCTPs in having only one signature sequence. Subsequently, SmTCTP was cloned in a T7 expression system and expressed as a histidine-tagged fusion protein. Recombinant SmTCTP (rSmTCTP) has a molecular mass of ∼23 kDa with the histidine tag. Further analysis showed that SmTCTP transcripts and protein are expressed in all life cycle stages of the parasite within the vertebrate hosts. Interestingly, antibodies to SmTCTP were present in the sera of mice 9 weeks after infection with S. mansoni. Characterization studies showed that rSmTCTP is a calcium-binding protein that can cause histamine release from basophil/mast cells and induce eosinophil infiltration. These findings suggest that SmTCTP may have an important role in the development of allergic inflammatory responses associated with schistosomiasis and may be a target for new drug development.

• Publication year

  2002

• Venue

  Journal of Biological Chemistry

• Publication date

  2002-08-23

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.M204114200PMID 12050167PMCID PMC2731418
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mechanisms of eosinophil accumulation around eggs ofSchistosoma japonicum: Role of two purified components, allergen and eosinophil chemotactic factor, from soluble egg antigens measured on sensitized guinea pig skin

  2004cited by this paper
• A novel Schistosoma mansoni G protein-coupled receptor is responsive to histamine.

  2002cited by this paper
• Molecular characterization of a calcium binding translationally controlled tumor protein homologue from the filarial parasites Brugia malayi and Wuchereria bancrofti.

  2002cited by this paper
• Recent investigations of artemether, a novel agent for the prevention of schistosomiasis japonica, mansoni and haematobia.

  2002cited by this paper
• Immunoprecipitation of [3H]Dihydroartemisinin Translationally Controlled Tumor Protein (TCTP) Adducts from Plasmodium falciparum-Infected Erythrocytes by Using Anti-TCTP Antibodies

  2001cited by this paper
• Immune mimicry in malaria: Plasmodium falciparum secretes a functional histamine-releasing factor homolog in vitro and in vivo

  2001cited by this paper
• Identification of some heat-induced genes of Trichinella spiralis

  2001cited by this paper
• Identification of the self-interaction of rat TCTP/IgE-dependent histamine-releasing factor using yeast two-hybrid system.

  2000cited by this paper
• Human recombinant histamine-releasing factor activates human eosinophils and the eosinophilic cell line, AML14-3D10.

  2000cited by this paper
• Identification of the calcium binding sites in translationally controlled tumor protein

  2000cited by this paper
• A cnidarian homologue of translationally controlled tumor protein (P23/TCTP)

  2000cited by this paper
• The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle.

  1999cited by this paper
• Identification of heavy metal induced changes in the expression patterns of the translationally controlled tumour protein (TCTP) in the earthworm Lumbricus rubellus1.

  1998cited by this paper
• Macrophage colony-stimulating factor stimulates synthesis and secretion of a mouse homolog of a human IgE-dependent histamine-releasing factor by macrophages in vitro and in vivo.

  1998cited by this paper
• The Plasmodium falciparum Translationally Controlled Tumor Protein Homolog and Its Reaction with the Antimalarial Drug Artemisinin*

  1998cited by this paper
• The PROSITE database, its status in 1997

  1997cited by this paper
• ICAM-1 and iNOS expression increased in the skin of mice after vaccination with gamma-irradiated cercariae of Schistosoma mansoni.

  1997cited by this paper
• Human recombinant histamine-releasing factor.

  1997cited by this paper
• Translationally controlled tumor protein: A protein identified in several nontumoral cells including erythrocytes

  1997cited by this paper
• Kinetic study of eosinophil chemotactic factor production with reference to eosinophilia and granuloma formation in mice infected with Schistosoma japonicum.

  1996cited by this paper
• Use of a new glass microfibre histamine release method to study the modulation of the host response in human schistosomiasis mansoni. Individuals with different degrees of exposure to the disease show differing antibody biological function

  1996cited by this paper
• Schistosoma mansoni egg-induced early IL-4 production is dependent upon IL-5 and eosinophils

  1996cited by this paper
• Human basophils release interleukin‐4 after stimulation with Schistosoma mansoni egg antigen

  1996cited by this paper
• An immunoglobulin E-dependent recombinant histamine-releasing factor induces interleukin-4 secretion from human basophils

  1996cited by this paper
• Secretion of an anti-inflammatory, immunomodulatory factor by Schistosomulae of Schistosoma mansoni.

  1996cited by this paper
• Molecular identification of an IgE-dependent histamine-releasing factor.

  1995cited by this paper
• Characterization and localization of Schistosoma mansoni calreticulin Sm58

  1994cited by this paper
• Immunochemical studies on the cercarial-specific calcium binding protein of Schistosoma mansoni

  1994cited by this paper
• Effector functions of eosinophils in schistosomiasis.

  1992cited by this paper
• An alfalfa cDNA encodes a protein with homology to translationally controlled human tumor protein

  1992cited by this paper
• The growth-related protein P23 of the Ehrlich ascites tumor: translational control, cloning and primary structure.

  1989cited by this paper
• Schistosoma mansoni: neurotransmitters and the mobility of cercariae and schistosomules.

  1985cited by this paper
• Immune responses and immunoregulation in relation to human schistosomiasis in Egypt. II. Cimetidine reversal of histamine-mediated suppression of antigen-induced blastogenesis.

  1984cited by this paper
• Recovery of schistosomula of Schistosoma mansoni from mouse skin: involvement of mast cells and vasoactive amines.

  1984cited by this paper
• Modulation of granulomatous hypersensitivity. V. Participation of histamine receptor positive and negative lymphocytes in the granulomatous response of Schistosoma mansoni-infected mice.

  1983cited by this paper
• Immunologic studies of human schistosomiasis. II. Interrelationship of serum and ascitic fluid histamine, IgE and total eosinophils in acute and chronic human schistosomiasis.

  1983cited by this paper
• Cercaria-induced histamine release: a factor in the pathogenesis of schistosome dermatitis?

  1980cited by this paper
• Modulation of the host response in human schistosomiasis. IV. Parasite antigen induces release of histamine that inhibits lymphocyte responsiveness in vitro.

  1979cited by this paper
• Eosinophil-enriched inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni.

  1976cited by this paper
• The allergens of Schistosoma mansoni. I. Initial separation and comparative assay.

  1973cited by this paper
• Plasma histamine level and its relation to blood eosinophilia in bilharzialcases.

  1970cited by this paper
• In Vitro Histamine Release from Blood Cellular Elements of Rabbits Infected with Schistosoma mansoni

  1968cited by this paper

• The TCTP is essential for ovarian development and oviposition of Rhipicephalus haemaphysaloides.

  2024cites this paper
• The multifaceted potential of TPT1 as biomarker and therapeutic target

  2024cites this paper
• How to train your myeloid cells: a way forward for helminth vaccines?

  2023cites this paper
• Sequence Analysis and Comparison of TCTP Proteins from Human Protozoan Parasites

  2022cites this paper
• Schistosome Secretomes: Running title: Blood fluke protein secretions.

  2022cites this paper
• A nematode effector inhibits plant immunity by preventing cytosolic free Ca2+ rise.

  2022cites this paper
• Effects of Sarcoptes scabiei Translationally Controlled Tumor Protein (TCTP) on Histamine Release and Degranulation of KU812 Cells

  2022cites this paper
• Schistosome immunomodulators

  2021cites this paper
• Advances in vaccine development for human trichuriasis

  2021cites this paper
• Behind Enemy Lines: Immunomodulatory Armamentarium of the Schistosome Parasite

  2020cites this paper
• Developing novel ways of studying motility in Schistosoma mansoni and its potential contribution towards inhibiting Schistosomiasis.

  2019cites this paper
• Homology modeling and molecular dynamics simulations of a cassava translationally controlled tumor protein (MeTCTP)

  2019cites this paper
• TCTP from Loxosceles Intermedia (Brown Spider) Venom Contributes to the Allergic and Inflammatory Response of Cutaneous Loxoscelism

  2019cites this paper
• Vaccination Against Whipworm: Identification of Potential Immunogenic Proteins in Trichuris muris Excretory/Secretory Material

  2018cites this paper
• Structural Insights into TCTP and Its Interactions with Ligands and Proteins.

  2017cites this paper
• A novel Meloidogyne enterolobii effector MeTCTP promotes parasitism by suppressing programmed cell death in host plants.

  2017influential citation
• The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation.

  2017cites this paper
• Synergistic Actions of Histamine-Releasing Factor and Histamine Releasing Factor-Reactive IgE in Chronic Urticaria

  2017cites this paper
• History of Histamine-Releasing Factor (HRF)/Translationally Controlled Tumor Protein (TCTP) Including a Potential Therapeutic Target in Asthma and Allergy.

  2017cites this paper
• In silico modeling and characterization of phytoparasitic nematodes translationally-controlled tumor proteins.

  2017cites this paper
• Expression of Translationally Controlled Tumor Protein in Human Kidney and in Renal Cell Carcinoma

  2015cites this paper
• Histamine Immunoreactive Elements in the Central and Peripheral Nervous Systems of the Snail, Biomphalaria spp., Intermediate Host for Schistosoma mansoni

  2015cites this paper
• Aberrant expression profile of translationally controlled tumor protein and tumor-suppressive microRNAs in cervical cancer.

  2015cites this paper
• Immunization of mice with Plasmodium TCTP delays establishment of Plasmodium infection

  2015cites this paper
• TCTP as therapeutic target in cancers.

  2014cites this paper
• Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening

  2014cites this paper
• Secretion of Translationally Controlled Tumor Protein/Histamine Releasing Factor and Its Relevance to Parasitic Infections

  2014influential citation
• Mechanisms of allergic diseases

  2013cites this paper
• Dimerization of TCTP and its clinical implications for allergy.

  2013cites this paper
• An EF-handed Ca2+-binding protein of Chinese liver fluke Clonorchis sinensis

  2013cites this paper
• Lymphocytes Histamine-releasing Factor in Human T the Human Recombinant Inhibition of Cytokine Gene Transcription By

  2013cites this paper
• The effect of Translationally Controlled Tumour Protein (TCTP) on programmed cell death in plants

  2013influential citation
• Molecular cloning, expression profiles and characterization of a novel translationally controlled tumor protein in rubber tree (Hevea brasiliensis)

  2013cites this paper
• Molecular cloning, expression profiles and characterization of a novel translationally controlled tumor protein in rubber tree (Hevea brasiliensis).

  2013cites this paper
• Genes Regulated in Plants under Salt Stress

  2013cites this paper
• Molecular identification, immunolocalization, and characterization of Clonorchis sinensis calmodulin

  2013cites this paper
• Molecular and biochemical characterization of tomato (Solanum lycopersicum L.) plants cv. Micro-Tom under lead (Pb)-induced stress

  2013cites this paper
• STEAP Proteins: From Structure to Applications in Cancer Therapy

  2012cites this paper
• Enhanced Basophil Reactivities during Severe Malaria and Their Relationship with the Plasmodium falciparum Histamine-Releasing Factor Translationally Controlled Tumor Protein

  2012cites this paper
• Sumoylation of Human Translationally Controlled Tumor Protein Is Important for Its Nuclear Transport

  2012cites this paper
• Histamine releasing factor/translationally controlled tumor protein: History, functions and clinical implications

  2012cites this paper
• Potential role of histamine releasing factor (HRF) as a therapeutic target for treating asthma and allergy

  2012cites this paper
• Translationally Controlled Tumour Protein (TCTP) is present in human cornea and increases in herpetic keratitis

  2012cites this paper
• Brown Spider (Loxosceles genus) Venom Toxins: Tools for Biological Purposes

  2011cites this paper
• Cloning and prokaryotic expression of translationally controlled tumor protein (TaTCTP) gene from wheat and preparation of antiserum

  2011cites this paper
• CARACTERIZAÇÃO MOLECULAR DO GENE QUE CODIFICA A TCTP (Translationally Controlled Tumor Protein) EM TOMATE (Solanum lycopersicum cv. Santa Clara)

  2011cites this paper
• Preparation and characterization of monoclonal antibody against translationally controlled tumor protein.

  2011cites this paper
• Physical and Functional Antagonism between Tumor Suppressor Protein p53 and Fortilin, an Anti-apoptotic Protein*

  2011cites this paper
• Manipulation of vascular function by blood flukes?

  2011cites this paper
• The Effects of Overexpression of Histamine Releasing Factor (HRF) in a Transgenic Mouse Model

  2010cites this paper
• A controlled trial to assess the effect of quinine, chloroquine, amodiaquine, and artesunate on Loa loa microfilaremia.

  2010cites this paper
• Molecular cloning and expression characterization of translationally controlled tumor protein in silkworm pupae

  2010cites this paper
• Neuronal signaling in schistosomes: current status and prospects for postgenomics

  2010cites this paper
• Basophils and Eosinophils

  2010cites this paper
• Identification of the housekeeping genes using cross experiments via in silico analysis.

  2010cites this paper
• Molecular cloning and expression of EgTCTP, encoding a calcium binding protein, enhances the growth of callus in oil palm (Elaeis guineensis, Jacq).

  2010cites this paper
• Characterization of novel biogenic amine receptors in the human bloodfluke «Schistosoma mansoni»

  2010cites this paper
• Estudo do papel da TCTP (Translationally Controlled Tumour Protein) na resposta ao estresses bióticos e abióticos em plantas

  2010cites this paper
• Histamine signalling in Schistosoma mansoni: immunolocalisation and characterisation of a new histamine-responsive receptor (SmGPR-2).

  2010cites this paper
• Histamine release factor from Dermanyssus gallinae (De Geer): characterization and in vitro assessment as a protective antigen.

  2009cites this paper
• Gene silencing of translationally controlled tumor protein (TCTP) by siRNA inhibits cell growth and induces apoptosis of human prostate cancer cells.

  2009cites this paper
• Analysis of the translationally controlled tumour protein in the nematodes Ostertagia ostertagi and Caenorhabditis elegans suggests a pivotal role in egg production.

  2009cites this paper
• Chapter 4. Peptidases of trematodes.

  2009cites this paper
• Expression profile, localization of an 8-kDa calcium-binding protein from Schistosoma japonicum (SjCa8), and vaccine potential of recombinant SjCa8 (rSjCa8) against infections in mice

  2009influential citation
• Alternaria alternata TCTP, a novel cross-reactive ascomycete allergen.

  2009cites this paper
• Translationally controlled tumor protein is a novel heat shock protein with chaperone-like activity.

  2009cites this paper
• Developmental expression analysis and immunolocalization of a biogenic amine receptor in Schistosoma mansoni.

  2009cites this paper
• Distinct characteristics of signal transduction events by histamine-releasing factor/translationally controlled tumor protein (HRF/TCTP)-induced priming and activation of human basophils.

  2008cites this paper
• Cladosporium herbarum translationally controlled tumor protein (TCTP) is an IgE-binding antigen and is associated with disease severity.

  2008cites this paper
• Identification of differentially expressed proteins in the heart of translationally controlled tumor protein over-expressing transgenic mice.

  2008cites this paper
• Praziquantel Affects the Regulatory Myosin Light Chain of Schistosoma mansoni

  2008cites this paper
• Messenger RNA expression of translationally controlled tumor protein (TCTP) in liver regeneration and cancer.

  2008cites this paper
• T(H)2 adjuvants: implications for food allergy.

  2008cites this paper
• Cloning, expression and characterization of translationally controlled tumor protein (TCTP) gene from flatfish turbot (Scophthalmus maximus)

  2008cites this paper
• Study of the physiological function of three putative modifiers of the p53 pathway: Nucleostemin, Ptprv and TCTP

  2008cites this paper
• Advances in the discovery and development of trematocidal drugs

  2007cites this paper
• Genetic Variability, Antigenicity and Antifungal Susceptibility of Madurella Mycetomatis

  2007cites this paper
• Translationally controlled tumor protein of Brugia malayi functions as an antioxidant protein

  2007cites this paper
• Immunological cross-reactivity analysis on recombinant histamine-releasing factors from Schistosoma japonicum, Clonorchis sinensis, and Wistar rat

  2007influential citation
• Fortilin binds Ca2+ and blocks Ca2+-dependent apoptosis in vivo.

  2007cites this paper
• The salivary glands and saliva of Anopheles gambiae as an essential step in the Plasmodium life cycle: A global proteomic study

  2007cites this paper
• Solution structure and mapping of a very weak calcium-binding site of human translationally controlled tumor protein by NMR.

  2007cites this paper
• Schistosomiasis—a century searching for chemotherapeutic drugs

  2006cites this paper
• Staying alive in adversity: transcriptome dynamics in the stress-resistant dauer larva

  2006influential citation
• Cloning and characterization of a high mobility group box 1 (HMGB1) homologue protein from Schistosoma mansoni.

  2006cites this paper
• Basophils, basophilia and helminth infections.

  2006cites this paper
• Translationally Controlled Tumor Protein from Madurella mycetomatis, a Marker for Tumorous Mycetoma Progression

  2006cites this paper
• Calcium homeostasis in human placenta: role of calcium-handling proteins.

  2006cites this paper
• The Molecular and Biological Analysis of Ixodid Ticks Histamine Release Factors

  2005cites this paper
• Stage-specifically expressed schistosome proteins as potential chemotherapeutic targets.

  2005cites this paper
• The Translationally Controlled Tumor Protein Is a Novel Calcium Binding Protein of the Human Placenta and Regulates Calcium Handling in Trophoblast Cells1

  2005cites this paper
• SCHISTOSOMA MANSONI DERMASEPTIN-LIKE PEPTIDE: STRUCTURAL AND FUNCTIONAL CHARACTERIZATION

  2005cites this paper
• Modulation of the host's immune response by schistosome larvae

  2005cites this paper
• Classical transmitters and their receptors in flatworms

  2005cites this paper
• Molecular and cellular profiles of insect bacteriocytes: mutualism and harm at the initial evolutionary step of symbiogenesis

  2004cites this paper
• cDNA Cloning of Translationally Controlled Tumor Protein/Histamine Releasing Factor (TCTP/HRF) from the Intertidal Harpacticoid Copepod Tigriopus japonicus

  2004cites this paper
• Molecular cloning and expression of a mammalian homologue of a translationally controlled tumor protein (TCTP) gene from Penaeus monodon shrimp.

  2004cites this paper
• The translationally controlled tumour protein (TCTP).

  2004cites this paper
• Parasite Antigen-Driven Basophils Are a Major Source of IL-4 in Human Filarial Infections

  2004cites this paper
• Basophils and immunity to parasites: an update

  2004cites this paper