Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli.

No abstract is available for this paper.

• Publication year

  1991

• Venue

  Gene

• Publication date

  1991-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0378-1119(91)90366-JPMID 2055470
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capability.

  1990cited by this paper
• Why Is Inequality Growing

  1990cited by this paper
• Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12

  1989cited by this paper
• Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

  1988cited by this paper
• Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.

  1988cited by this paper
• Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.

  1988cited by this paper
• [58] Direct sequencing of denatured plasmid DNA

  1987cited by this paper
• Direct sequencing of denatured plasmid DNA.

  1987cited by this paper
• XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection.

  1987cited by this paper
• Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering.

  1986cited by this paper
• A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

  1985cited by this paper
• Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

  1985cited by this paper
• Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.

  1984cited by this paper
• Complete sequence of pSC101.

  1984cited by this paper
• The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

  1982cited by this paper
• Versatile low-copy-number plasmid vectors for cloning in Escherichia coli.

  1982cited by this paper
• Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12

  1980cited by this paper
• Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome

  1980cited by this paper
• Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages λtna

  1979cited by this paper
• Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2.

  1979cited by this paper
• Characterization of lambdapolA transducing phages; effective expression of the E. coli polA gene.

  1979cited by this paper
• Construction of biologically functional bacterial plasmids in vitro.

  1973cited by this paper

• A prophage-encoded abortive infection protein preserves host and prophage spread.

  2026cites this paper
• YeiE regulates YeiH to implement sulfite stress resistance in Salmonella enterica serotype Typhimurium

  2026cites this paper
• LeuO fine-tunes the virulence attributable to Salmonella pathogenicity island-2 via repressing ssrA transcription in Salmonella Typhimurium

  2026cites this paper
• Bacterial species and surface structures shape gene transfer and the transcriptional landscape during active conjugation

  2025cites this paper
• Probiotic biogeography and sepsis prevention in the neonatal intestine

  2025cites this paper
• Substrate specificity of lipid A 4′-phosphatase in Bacteroides thetaiotaomicron

  2025cites this paper
• Intranasal vaccination with probiotic Escherichia coli membrane vesicles displaying pneumococcal capsular polysaccharides elicits mucosal immunity

  2025cites this paper
• The novel Salmonella effector STM2610 promotes epithelial cell invasion and in vivo virulence.

  2025cites this paper
• Macrolide resistance due to erm(55)

  2025influential citation
• A Gifsy prophage-encoded protein confers broad phage resistance in Salmonella enterica and is widely distributed across Enterobacteriaceae

  2025cites this paper
• Fatty acid metabolism governed by FadR is essential for Cronobacter sakazakii survival in dry food matrices and neonatal infection.

  2025cites this paper
• Mechanistic basis of antimicrobial resistance mediated by the phosphoethanolamine transferase MCR-1

  2025cites this paper
• O2-dependent incapacitation of the Salmonella pathogenicity island 1 repressor HilE

  2025influential citation
• Amino acid decarboxylation preserves Salmonella fitness during phagocyte-derived oxidative stress

  2025cites this paper
• SLC11A2 affects nutritional immunity in the gut epithelium

  2025cites this paper
• Smart culture medium optimization for recombinant protein production: Experimental, modeling, and AI/ML-driven strategies.

  2025cites this paper
• Pectobacterium atrosepticum SCRI1043 flagella mediate adherence to potato plants indirectly through motility

  2025cites this paper
• The two-component system CpxAR controls biofilm formation by directly regulating the T3SS needle tip protein EseB in Edwardsiella piscicida

  2025cites this paper
• YeiE regulates YeiH to implement sulfite stress resistance in Salmonella enterica serotype Typhimurium

  2025cites this paper
• Siderophore systems at play: system interactions as drivers of diversification in pathogenic Vibrio

  2025cites this paper
• Replicon family of Vibrionaceae plasmids as a reservoir of antimicrobial and phage resistance genes in marine ecosystems

  2025cites this paper
• The co-localizing Zorya II, Druantia III, and ARMADA II defense systems on O-island 172 confer synergistic anti-phage defense in enterohemorrhagic Escherichia coli

  2025cites this paper
• Microbes vary strategically in their metalation of mononuclear enzymes

  2024cites this paper
• Destruction of the brush border by Salmonella enterica sv. Typhimurium subverts resorption by polarized epithelial cells

  2024cites this paper
• Bacterial suppressor-of-copper-sensitivity proteins exhibit diverse thiol-disulfide oxidoreductase cellular functions

  2024cites this paper
• Generation of a plasmid series for rapid sub-cloning and use in various Enterobacteriaceae.

  2024cites this paper
• T3SS protein EsrC binds to the lacI-like operator of type 1 fimbrial operon to suppress adhesion of Edwardsiella piscicida

  2024cites this paper
• Mucosal adjuvanticity and mucosal booster effect of colibactin-depleted probiotic Escherichia coli membrane vesicles

  2024cites this paper
• A bacterial methyltransferase that initiates biotin synthesis, an attractive anti-ESKAPE druggable pathway

  2024cites this paper
• High fat intake sustains sorbitol intolerance after antibiotic-mediated Clostridia depletion from the gut microbiota.

  2024cites this paper
• Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria

  2024cites this paper
• The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate

  2024cites this paper
• Subversion of a family of antimicrobial proteins by Salmonella enterica

  2024cites this paper
• In-patient evolution of a high-persister Escherichia coli strain with reduced in vivo antibiotic susceptibility

  2024cites this paper
• Effector Translocation Assay: Differential Solubilization.

  2024cites this paper
• Non‐canonical amino acid bioincorporation into antimicrobial peptides and its challenges

  2024cites this paper
• Engineering Escherichia coli for constitutive production of monophosphoryl lipid A vaccine adjuvant

  2024cites this paper
• Anaerobic respiration of host-derived methionine sulfoxide protects intracellular Salmonella from the phagocyte NADPH oxidase.

  2024cites this paper
• The Sigma Factor AsbI Is Required for the Expression of Acinetobactin Siderophore Transport Genes in Aeromonas salmonicida

  2023cites this paper
• Shigella flexneri Adapts to Niche-Specific Stresses through Modifications in Cell Envelope Composition and Decoration.

  2023cites this paper
• Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection

  2023cites this paper
• Apoptotic signaling clears engineered Salmonella in an organ-specific manner

  2023cites this paper
• (p)ppGpp is required for virulence of Shigella flexneri

  2023cites this paper
• Arginine Metabolism Powers Salmonella Resistance to Oxidative Stress

  2023cites this paper
• Manipulation of microvillar proteins during Salmonella enterica invasion results in brush border effacement and actin remodeling

  2023cites this paper
• Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid

  2023cites this paper
• Gre factors help Salmonella adapt to oxidative stress by improving transcription elongation and fidelity of metabolic genes

  2023cites this paper
• Bacterial suppressor-of-copper-sensitivity (Scs) proteins exhibit diverse thiol-disulfide oxidoreductase cellular functions

  2023cites this paper
• The methylglyoxal pathway is a sink for glutathione in Salmonella experiencing oxidative stress

  2023cites this paper
• Nonredundant Dimethyl Sulfoxide Reductases Influence Salmonella enterica Serotype Typhimurium Anaerobic Growth and Virulence

  2023cites this paper
• Metabolic engineering of Escherichia coli to efficiently produce monophosphoryl lipid A

  2023cites this paper
• Modulation of Salmonella virulence by a novel SPI-2 injectisome effector that interacts with the dystrophin-associated protein complex

  2023cites this paper
• Overexpression of T3SS translocation signals in Salmonella causes delayed attenuation

  2023cites this paper
• Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection.

  2023cites this paper
• Remodulation of bacterial transcriptome after acquisition of foreign DNA: the case of irp-HPI high-pathogenicity island in Vibrio anguillarum

  2023cites this paper
• A ToxIN homolog from Salmonella enterica serotype Enteritidis impairs bacteriophage infection.

  2023cites this paper
• Silencing Transcription from an Influenza Reverse Genetics Plasmid in E. coli Enhances Gene Stability

  2023cites this paper
• Apoptotic signaling clears engineered Salmonella in an organ-specific manner

  2023cites this paper
• Two Polyketides Intertwined in Complex Regulation: Posttranscriptional CsrA-Mediated Control of Colibactin and Yersiniabactin Synthesis in Escherichia coli

  2022cites this paper
• rumAB genes from SXT/R391 ICEs confer UV-induced mutability to Proteus mirabilis hosts and improve conjugation after UV irradiation.

  2022cites this paper
• Detection of a disulphide bond and conformational changes in Shigella flexneri Wzy, and the role of cysteine residues in polymerase activity.

  2022cites this paper
• The C nucleotide at the mature 5′ end of the Escherichia coli proline tRNAs is required for the RNase E cleavage specificity at the 3′ terminus as well as functionality

  2022cites this paper
• Defects in DNA double‐strand break repair resensitize antibiotic‐resistant Escherichia coli to multiple bactericidal antibiotics

  2022cites this paper
• Salmonella Typhimurium lacking phoBR as a live vaccine candidate against poultry infection.

  2022cites this paper
• Integration of the Salmonella Typhimurium Methylome and Transcriptome Reveals That DNA Methylation and Transcriptional Regulation Are Largely Decoupled under Virulence-Related Conditions

  2022cites this paper
• Colonocyte-derived lactate promotes E. coli fitness in the context of inflammation-associated gut microbiota dysbiosis

  2022cites this paper
• A highly immunogenic vaccine platform against encapsulated pathogens using chimeric probiotic Escherichia coli membrane vesicles

  2022cites this paper
• Capsular polysaccharide inhibits vaccine-induced O-antigen antibody binding and function across both classical and hypervirulent K2:O1 strains of Klebsiella pneumoniae

  2022cites this paper
• Transcriptional insight into the effect of benzalkonium chloride on resistance and virulence potential in Salmonella Typhimurium.

  2022cites this paper
• Transposon-Directed Insertion-Site Sequencing Reveals Glycolysis Gene gpmA as Part of the H2O2 Defense Mechanisms in Escherichia coli

  2022cites this paper
• Bacteroides thetaiotaomicron Outer Membrane Vesicles Modulate Virulence of Shigella flexneri

  2022cites this paper
• Identification of the Shigella flexneri Wzy Domain Modulating WzzpHS-2 Interaction and Detection of the Wzy/Wzz/Oag Complex

  2022cites this paper
• Cryo-EM Structure of the Type IV Pilus Extension ATPase from Enteropathogenic Escherichia coli

  2022cites this paper
• Processing of the alaW alaX operon encoding the Ala2 tRNAs in Escherichia coli requires both RNase E and RNase P

  2022cites this paper
• A Robust One-Step Recombineering System for Enterohemorrhagic Escherichia coli

  2022cites this paper
• Oxidative stress activates transcription of Salmonella pathogenicity island-2 genes in macrophages

  2022cites this paper
• Persistent Salmonella infections in humans are associated with mutations in the BarA/SirA regulatory pathway

  2022cites this paper
• Analysis of Salmonella Typhi Pathogenesis in a Humanized Mouse Model.

  2022cites this paper
• Maturation of the E. coli Glu2, Ile1, and Ala1B tRNAs utilizes a complex processing pathway

  2022cites this paper
• Rescue of transcriptional pausing in metabolic genes jumpstarts Salmonella antioxidant defenses

  2022cites this paper
• Identification of Key Functions Required for Production and Utilization of the Siderophore Piscibactin Encoded by the High-Pathogenicity Island irp-HPI in Vibrionaceae

  2022cites this paper
• Human variation impacting MCOLN2 restricts Salmonella Typhi replication by magnesium deprivation

  2022cites this paper
• Non-redundant dimethyl sulfoxide reductases influence Salmonella enterica serotype Typhimurium anaerobic growth and virulence

  2022cites this paper
• Interdependence of Shigella flexneri O Antigen and Enterobacterial Common Antigen Biosynthetic Pathways

  2022cites this paper
• Subcellular localization of the enterobacterial common antigen GT‐E‐like glycosyltransferase, WecG

  2022cites this paper
• Escherichia coli Uses a Dedicated Importer and Desulfidase To Ferment Cysteine

  2022cites this paper
• Co-production of Tet(X) and MCR-1, Two Resistance Enzymes by A Single Plasmid.

  2021cites this paper
• Natural Transformation in a Classical-Biotype Vibrio cholerae Strain

  2021cites this paper
• The ABC-Type Efflux Pump MacAB Is Involved in Protection of Serratia marcescens against Aminoglycoside Antibiotics, Polymyxins, and Oxidative Stress

  2021cites this paper
• Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC

  2021cites this paper
• Genomic and phenotypic analysis of SspH1 identifies a new Salmonella effector, SspH3

  2021cites this paper
• The Temperature-Dependent Expression of the High-Pathogenicity Island Encoding Piscibactin in Vibrionaceae Results From the Combined Effect of the AraC-Like Transcriptional Activator PbtA and Regulatory Factors From the Recipient Genome

  2021cites this paper
• Integration of the Salmonella Typhimurium methylome and transcriptome following environmental or metabolic perturbation reveals DNA methylation and transcriptional regulation are largely decoupled

  2021cites this paper
• Diacylglycerol Kinase A Is Essential for Polymyxin Resistance Provided by EptA, MCR-1, and Other Lipid A Phosphoethanolamine Transferases

  2021cites this paper
• Engineering Escherichia coli to produce Bordetella pertussis oligosaccharide with multiple trisaccharide units.

  2021cites this paper
• The vulnerable versatility of Salmonella antibiotic persisters during infection.

  2021cites this paper
• C4-dicarboxylates and L-aspartate utilization by Escherichia coli K-12 in the mouse intestine: L-aspartate as a major substrate for fumarate respiration and as a nitrogen source.

  2021cites this paper
• The Mutant βE202K Sliding Clamp Protein Impairs DNA Polymerase III Replication Activity

  2021cites this paper
• Elevated Levels of the Escherichia coli nrdAB-Encoded Ribonucleotide Reductase Counteract the Toxicity Caused by an Increased Abundance of the β Clamp

  2021cites this paper
• Vibrio neptunius Produces Piscibactin and Amphibactin and Both Siderophores Contribute Significantly to Virulence for Clams

  2021cites this paper