Identification of the Key Protein for Zinc Uptake in Hemophilus influenzae *

Very little is known about specific mechanisms for zinc accumulation and transport in bacteria. In this study a putative adhesin B in Hemophilus influenzae, the product of gene HI0119, has been identified as a periplasmic zinc-binding protein (PZP1). A pzp1-deficient mutant has been constructed which is defective for growth under aerobic conditions and grows poorly under anaerobic conditions. The growth defect is specifically rescued by supplementing the growth medium with high concentrations of zinc. Subcellular fractionation was used to localize PZP1 to the periplasmic region in a nontypeable H. influenzae strain and in a transfected recombinant Escherichia coli strain (TApzp1). Recombinant PZP1, purified from a periplasmic extract of E. coli strain TApzp1, contained ∼two zinc atoms/protein molecule as determined by neutron activation analysis and atomic absorption spectroscopy. The zinc atoms could be removed by incubation with EDTA, and, by further addition of zinc, a total of five zinc atoms/PZP1 could be bound. Direct binding of 65Zn to the recombinant protein by Western blot was demonstrated. Taken together, these results provide direct evidence that PZP1 plays a key role in zinc uptake by H. influenzae.

• Publication year

  1997

• Venue

  Journal of Biological Chemistry

• Publication date

  1997-11-14

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.272.46.29033PMID 9360976
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• The yeast ZRT1 gene encodes the zinc transporter protein of a high-affinity uptake system induced by zinc limitation.

  1996cited by this paper
• A novel iron-regulated metal transporter from plants identified by functional expression in yeast.

  1996cited by this paper
• The ZRT2 Gene Encodes the Low Affinity Zinc Transporter in Saccharomyces cerevisiae*

  1996cited by this paper
• Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae

  1996cited by this paper
• Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.

  1995cited by this paper
• The fimA locus of Streptococcus parasanguis encodes an ATP‐binding membrane transport system

  1995cited by this paper
• FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis

  1995cited by this paper
• Zinc finger diversity

  1994cited by this paper
• Isolation and purification of periplasmic binding proteins.

  1994cited by this paper
• The biochemical basis of zinc physiology.

  1993cited by this paper
• Overexpression and purification of a fimbria-associated adhesin of Streptococcus parasanguis

  1993cited by this paper
• Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria

  1993cited by this paper
• Gene regulation of plasmid- and chromosome-determined inorganic ion transport in bacteria.

  1992cited by this paper
• ABC transporters: from microorganisms to man.

  1992cited by this paper
• Genetic systems in Haemophilus influenzae.

  1991cited by this paper
• Molecular characterization of the pathogen-specific, 34-kilodalton membrane immunogen of Treponema pallidum

  1989cited by this paper
• Nucleotide sequence analysis of a type 1 fimbrial gene of Streptococcus sanguis FW213

  1989cited by this paper
• Characterization of a zinc blotting technique: evidence that a retroviral gag protein binds zinc.

  1988cited by this paper
• Bacterial periplasmic transport systems: structure, mechanism, and evolution.

  1986cited by this paper
• The pathogenicity of Haemophilus influenzae.

  1984cited by this paper
• Phase separation of integral membrane proteins in Triton X-114 solution.

  1981cited by this paper
• Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

  1979cited by this paper
• Energy-dependent zinc transport by escherichia coli.

  1974cited by this paper
• Sporulation of Bacillus megaterium: roles of metal ions.

  1971cited by this paper
• Sporulation of Bacillus megaterium: roles of metal ions.

  1971cited by this paper
• Interrelationships between the utilization of magnesium and the uptake of other bivalent cations by bacteria.

  1970cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• Structural and biochemical characterization of Acinetobacter baumannii ZnuA.

  2022cites this paper
• Conformational flexibility in the zinc solute-binding protein ZnuA

  2022cites this paper
• Structure and Function of the Zinc Binding Protein ZrgA from Vibrio cholerae

  2022cites this paper
• Mechanisms of Kwashiorkor-Associated Immune Suppression: Insights From Human, Mouse, and Pig Studies

  2022cites this paper
• Periplasmic-binding protein-based biosensors and bioanalytical assay platforms: Advances, considerations, and strategies for optimal utility

  2021cites this paper
• Structural basis for zinc-induced activation of a zinc uptake transcriptional regulator

  2021cites this paper
• Specificity of Interactions between Components of Two Zinc ABC Transporters in Paracoccus denitrificans

  2020cites this paper
• The structure and metal binding properties of Chlamydia trachomatis YtgA 1

  2019cites this paper
• Structure and Metal Binding Properties of Chlamydia trachomatis YtgA

  2019cites this paper
• Resistance to Metals Used in Agricultural Production

  2019cites this paper
• Two ABC Transporters and a Periplasmic Metallochaperone Participate in Zinc Acquisition in Paracoccus denitrificans

  2018cites this paper
• Resistance to Metals Used in Agricultural Production

  2018cites this paper
• Control de la homeostasis de zinc y su rol en la virulencia de Salmonella

  2017cites this paper
• Relationships between zinc and meropenem resistance in the natural environment and experimental bioreactors

  2016cites this paper
• High-Throughput Detection of Thiamine Using Periplasmic Binding Protein-Based Biorecognition.

  2016cites this paper
• Zinc acquisition via ZnuABC in Yersinia pseudotuberculosis facilitates resistance to oxidative stress

  2016cites this paper
• Characterisation of the ATP-binding cassette transporters of Pseudomonas aeruginosa

  2015influential citation
• Dual Zinc Transporter Systems in Vibrio cholerae Promote Competitive Advantages over Gut Microbiome

  2015cites this paper
• ZnuA and zinc homeostasis in Pseudomonas aeruginosa

  2015cites this paper
• Manganese uptake and streptococcal virulence

  2015cites this paper
• Mechanism of zinc resistance in a plant growth promoting Pseudomonas fluorescens strain

  2014cites this paper
• Control of zinc homeostasis in Agrobacterium tumefaciens via zur and the zinc uptake genes znuABC and zinT.

  2014cites this paper
• Mechanism of zinc resistance in a plant growth promoting Pseudomonas fluorescens strain

  2014influential citation
• Periplasmic binding protein-based detection of maltose using liposomes: a new class of biorecognition elements in competitive assays.

  2013cites this paper
• Zinc Competition among the Intestinal Microbiota

  2012cites this paper
• Evaluación de la capacidad de biorremoción de zinc en agua sintética de dos consorcios microbianos nativos, a escala de laboratorio

  2011cites this paper
• A Novel Zinc Binding System, ZevAB, Is Critical for Survival of Nontypeable Haemophilus influenzae in a Murine Lung Infection Model

  2011cites this paper
• Roles of Secreted Virulence Factors in Pathogenicity of Haemophilus Influenzae: A Dissertation

  2011influential citation
• The Zur-Regulated ZinT Protein Is an Auxiliary Component of the High-Affinity ZnuABC Zinc Transporter That Facilitates Metal Recruitment during Severe Zinc Shortage

  2010cites this paper
• AdcAII, a new pneumococcal Zn-binding protein homologous with ABC transporters: biochemical and structural analysis.

  2008cites this paper
• A Campylobacter jejuni znuA Orthologue Is Essential for Growth in Low-Zinc Environments and Chick Colonization

  2008cites this paper
• Attenuated Salmonella enterica serovar Typhimurium lacking the ZnuABC transporter confers immune-based protection against challenge infections in mice.

  2008cites this paper
• The Periplasmic Disulfide Oxidoreductase DsbA Contributes to Haemophilus influenzae Pathogenesis

  2008cites this paper
• High-Affinity Zn2+ Uptake System ZnuABC Is Required for Bacterial Zinc Homeostasis in Intracellular Environments and Contributes to the Virulence of Salmonella enterica

  2007cites this paper
• Identification and Characterization of Zn(II)-responsive Genes and Proteins in E. coli

  2007cites this paper
• The general transition metal (Tro) and Zn2+ (Znu) transporters in Treponema pallidum: analysis of metal specificities and expression profiles

  2007cites this paper
• Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli

  2007cites this paper
• Crystal structure of the zinc-binding transport protein ZnuA from Escherichia coli reveals an unexpected variation in metal coordination.

  2007cites this paper
• Microbial Genomics as an Integrated Tool for Developing Biosensors for Toxic Trace Elements in the Environment

  2005cites this paper
• Periplasmic competition for zinc uptake between the metallochaperone ZnuA and Cu,Zn superoxide dismutase

  2004cites this paper
• Identification of Actinobacillus pleuropneumoniae Genes Important for Survival during Infection in Its Natural Host

  2003cites this paper
• Issues underlying use of biosensors to measure metal bioavailability.

  2003cites this paper
• Zn(II) metabolism in prokaryotes.

  2003cites this paper
• Microbial Growth and Nutrition in the Host

  2002cites this paper
• A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae

  2002cites this paper
• Role of the High-Affinity Zinc Uptake znuABC System in Salmonella enterica Serovar Typhimurium Virulence

  2002cites this paper
• A new family of high-affinity ABC manganese and zinc permeases.

  2001cites this paper
• Identification and characterization of a high-affinity zinc uptake system in Neisseria gonorrhoeae.

  2001cites this paper
• Bacterial zinc transporters and regulators

  2001influential citation
• A Genomic Sample Sequence of the Entomopathogenic Bacterium Photorhabdus luminescens W14: Potential Implications for Virulence

  2000cites this paper
• Evidence for the transport of zinc(II) ions via the pit inorganic phosphate transport system in Escherichia coli.

  2000cites this paper
• Characterization of P1-deficient isogenic mutant of Haemophilus influenzae biogroup aegyptius associated with Brazilian purpuric fever.

  2000cites this paper
• The distribution of elements in cells

  2000cites this paper
• ATPases Families of Soft-Metal-Ion-Transporting

  1999cites this paper
• Characterisation of a new operon encoding a Zur-like protein and an associated ABC zinc permease in Listeria monocytogenes.

  1999cites this paper
• Families of Soft-Metal-Ion-Transporting ATPases

  1999cites this paper
• Identification and characterization of a Streptococcus pyogenes ABC transporter with multiple specificity for metal cations

  1999cites this paper
• Identification of the znuA-Encoded Periplasmic Zinc Transport Protein of Haemophilus ducreyi

  1999cites this paper
• Physicochemical Evidence that Treponema pallidum TroA Is a Zinc-Containing Metalloprotein That Lacks Porin-Like Structure

  1999cites this paper
• Microbial heavy-metal resistance

  1999cites this paper
• Identification of a Zinc-Specific Metalloregulatory Protein, Zur, Controlling Zinc Transport Operons inBacillus subtilis

  1998cites this paper
• An SmtB-like repressor from Synechocystis PCC 6803 regulates a zinc exporter.

  1998cites this paper
• The ZnuABC high‐affinity zinc uptake system and its regulator Zur in Escherichia coli

  1998cites this paper