Xenopus transcription factor A requires zinc for binding to the 5 S RNA gene.

Transcription factor A from immature Xenopus oocytes is found associated with 5 S RNA in a 7 S nucleoprotein complex. Atomic absorption analysis of EDTA-dialyzed 7 S particles reveals 2 mol of zinc/mol of particle. Factor A obtained from EDTA-dialyzed particles binds specifically to the 5 S RNA gene as determined by DNase I footprinting. Factor A alone, obtained by RNase digestion of the 7 S particle, contains zinc when dialyzed in the absence of EDTA. However, the zinc bound to free factor A is removed by dialysis against a buffer containing EDTA. The apoprotein does not bind to the 5 S RNA gene. Inhibition of footprinting is also effected by addition of EDTA to factor A without prolonged dialysis. Under these conditions, specific DNA binding ability is restored following addition of zinc. 1,10-Phenanthroline also inhibits binding of factor A to the intragenic control region of the 5 S RNA gene. In addition, this reagent specifically inhibits factor A-dependent synthesis of 5 S RNA but not factor A-independent tRNA synthesis in a HeLa cell in vitro transcription system.

• Publication year

  1983

• Venue

  Journal of Biological Chemistry

• Publication date

  1983-12-10

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(17)43831-2PMID 6196359
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cooperative model for the binding of Xenopus transcription factor A to the 5S RNA gene.

  1983cited by this paper
• Products of DNA cleavage by the 1,10-phenanthroline-copper complex. Inhibitors of Escherichia coli DNA polymerase I.

  1982cited by this paper
• Direct coordination of nucleotide with the intrinsic metal in Escherichia coli RNA polymerase. A nuclear magnetic resonance study with cobalt-substituted enzyme.

  1982cited by this paper
• Contact points between a positive transcription factor and the Xenopus 5S RNA gene.

  1982cited by this paper
• Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.

  1981cited by this paper
• The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.

  1981cited by this paper
• A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.

  1980cited by this paper
• A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.

  1980cited by this paper
• Degradation of deoxyribonucleic acid by a 1,10-phenanthroline-copper complex: the role of hydroxyl radicals.

  1980cited by this paper
• Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.

  1980cited by this paper
• A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.

  1980cited by this paper
• DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.

  1980cited by this paper
• Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex.

  1979cited by this paper
• Oxygen-dependent cleavage of DNA by the 1,10-phenanthroline . cuprous complex. Inhibition of Escherichia coli DNA polymerase I.

  1979cited by this paper
• The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.

  1979cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Metal ion-mediated specific interactions between nucleic acid bases of polynucleotides and amino acid side chains of polypeptides.

  1975cited by this paper

• Genome-Wide Analysis of the PLATZ Gene Family in Oryza Genus: Evolution, Expression During Inflorescence Development and Stress Responses

  2025cites this paper
• Precision gene editing: The power of CRISPR-Cas in modern genetics

  2025cites this paper
• The Arcana of Zinc

  2025cites this paper
• Comprehensive analysis of PLATZ family genes and their responses to abiotic stresses in Barley

  2024cites this paper
• Structure and RNA-binding of the helically extended Roquin CCCH-type zinc finger

  2024cites this paper
• Domain architecture and protein–protein interactions regulate KDM5A recruitment to the chromatin

  2023cites this paper
• Fe-S clusters masquerading as zinc finger proteins.

  2022cites this paper
• Quantitative elemental imaging in eukaryotic algae

  2022cites this paper
• Zn2+-Induced Conformational Change Affects the SAM Binding in a Mycobacterial SAM-Dependent Methyltransferase

  2022cites this paper
• Interactions of zinc- and redox-signaling pathways

  2021cites this paper
• Targeting Zinc Finger Proteins with Exogenous Metals and Molecules: Lessons learned from Tristetraprolin, a CCCH type Zinc Finger.

  2021cites this paper
• Theoretical, spectroscopic study about evaluation of trace elements (zinc and cadmium) µmol/L for pregnant women of age bearing during trimester

  2021cites this paper
• Ethical considerations of gene editing and genetic selection

  2020cites this paper
• Nutritional Intake Influences Zinc Levels in Preterm Newborns: An Observational Study

  2020cites this paper
• Zinc at the crossroads of exercise and proteostasis

  2020cites this paper
• Bioaccumulation of Metals/Metalloids and Histological and Immunohistochemical Changes in the Tissue of the European Hake, Merluccius merluccius (Linnaeus, 1758) (Pisces: Gadiformes: Merlucciidae), for Environmental Pollution Assessment

  2020cites this paper
• Sheep zinc finger proteins 395 (ZNF395): insertion/deletion variations, associations with growth traits, and mRNA expression

  2020cites this paper
• Step by step evolution of Indeterminate Domain (IDD) transcriptional regulators: from algae to angiosperms.

  2020cites this paper
• Regulation of the Central Dogma through Bioinorganic Events with Metal Coordination for Specific Interactions

  2020cites this paper
• CHAPTER 2 C H Zinc Fingers As DNA Binding Domains

  2020cites this paper
• EXPANDING THE SCOPE OF HETEROGENEOUS BACKBONE DESIGNS TO INCLUDE SINGLE- AND MULTI-DOMAIN ZINC FINGER PROTEINS

  2019cites this paper
• The redox biology of redox-inert zinc ions.

  2019cites this paper
• Human adenovirus – host cell interplay The role of the cellular zinc finger proteins and mitochondrial DNA

  2019cites this paper
• A novel SWIM domain protein ZSWIM5 inhibits the malignant progression of non-small-cell lung cancer

  2018cites this paper
• Lipidome of plasma lipoproteins and liver is zinc- modulated in High fat diet treated mice.

  2018cites this paper
• Promoters of Dengue Virus Complete Genome

  2018cites this paper
• A single C4 Zinc finger‐containing protein from Litopenaeus vannamei involved in antibacterial responses

  2018cites this paper
• Functional characterization of the biological significance of the ZBED6/ZC3H11A locus in placental mammals

  2017cites this paper
• Zinc Finger-Containing Cellular Transcription Corepressor ZBTB25 Promotes Influenza Virus RNA Transcription and Is a Target for Zinc Ejector Drugs

  2017cites this paper
• From Sequence to Function: Coevolving Amino Acids Encode Structural and Functional Domains

  2017cites this paper
• Patterns of coevolving amino acids unveil structural and dynamical domains

  2017cites this paper
• Structural Analyses of Zinc Finger Domains for Specific Interactions with DNA.

  2016cites this paper
• Effects of zinc fortification on the plasma fatty acid composition of beninese school children: A randomised, double-blind controlled trial

  2016cites this paper
• Revisiting and re-engineering the classical zinc finger peptide: consensus peptide-1 (CP-1).

  2016cites this paper
• EFFECT OF CHELATING AGENTS AND ARBUSCULAR MYCORRHIZA ON USE EFFICIENCY OF ZINC IN WINTER MAIZE - DIRECT SEEDED RICE SEQUENCE

  2016cites this paper
• Potassium Versus Sodium Selectivity in Monovalent Ion Channel Selectivity Filters.

  2016cites this paper
• Establishing the Relationship Between Function and Dynamics Within the Gated Mechanism of D-arginine Dehydrogenase

  2016cites this paper
• Oxidation Protection in Metal-Binding Peptide Motif and Its Application to Antibody for Site-Selective Conjugation

  2016cites this paper
• Functional Genomics of Symbiotic Nitrogen Fixation in Legumes with a Focus on Transcription Factors and Membrane Transporters

  2015cites this paper
• Characterisation of cello : an ENU-induced mouse model of early-onset hearing loss

  2015cites this paper
• Zinc Binding Activity of Human Metapneumovirus M2-1 Protein Is Indispensable for Viral Replication and Pathogenesis In Vivo

  2015cites this paper
• Analytical Techniques Using ICP-MS for Clinical and Biological Analysis

  2015cites this paper
• Zinc in Early Life: A Key Element in the Fetus and Preterm Neonate

  2015cites this paper
• Artificial Zinc Finger DNA Binding Domains: Versatile Tools for Genome Engineering and Modulation of Gene Expression

  2015cites this paper
• Fishy Business: Effect of Omega-3 Fatty Acids on Zinc Transporters and Free Zinc Availability in Human Neuronal Cells

  2014cites this paper
• Comparative thermodynamic analysis of zinc binding to the His/Cys motif in virion infectivity factor.

  2014cites this paper
• Zinc and the zinc proteome.

  2013cites this paper
• Emerging roles of zinc finger proteins in regulating adipogenesis

  2013cites this paper
• Criteria for an updated Classification of Human transcription factor DNA-binding Domains

  2013cites this paper
• A recessive X-linked mutation causes a threefold reduction of total body zinc accumulation in Drosophila melanogaster laboratory strains☆

  2013cites this paper
• Reactions of cadmium(II) nitrate with 4-(trimethylammonio)benzenethiolate in the presence of N-donor ligands.

  2012cites this paper
• Zinc and skin health: an overview

  2012cites this paper
• Thermodynamics of Zn2+ binding to Cys2His2 and Cys2HisCys zinc fingers and a Cys4 transcription factor site.

  2012cites this paper
• Zinc complexes developed as metallopharmaceutics for treating diabetes mellitus based on the bio-medicinal inorganic chemistry.

  2012cites this paper
• Group 12 metal zwitterionic thiolate compounds: preparation and structural characterization.

  2012cites this paper
• Getting a Tight Grip on DNA: Optimizing Zinc Fingers for Efficient ZFN-Mediated Gene Editing: A Dissertation

  2012cites this paper
• Nutritional Deficiencies and Phospholipid Metabolism

  2011cites this paper
• Escherichia coli topoisomerase I is an iron and zinc binding protein

  2011cites this paper
• Estudo do efeito de radiação ionizante em metaloproteínas que ligam DNA

  2011cites this paper
• Cysteine and histidine shuffling: mixing and matching cysteine and histidine residues in zinc finger proteins to afford different folds and function.

  2011cites this paper
• Serum Zinc Concentrations in Cystic Fibrosis Patients With Ages Above Four Years : A cross-sectional evaluation

  2011cites this paper
• The discovery of zinc fingers and their applications in gene regulation and genome manipulation.

  2010cites this paper
• Functional characterization of iron-substituted neural zinc finger factor 1: metal and DNA binding

  2010cites this paper
• Zinc Finger Proteins: From Atomic Contact to Cellular Function

  2010cites this paper
• The discovery of zinc fingers and their development for practical applications in gene regulation and genome manipulation

  2010cites this paper
• Cytosolic zinc buffering and muffling: their role in intracellular zinc homeostasis.

  2010cites this paper
• Ethanol-related teratogenicity and neurobehavioural impairments: influence of dietary zinc supplementation during pregnancy.

  2009cites this paper
• The role of zinc in preventing fetal dysmorphology and brain injury mediated by maternal exposure to infection in pregnancy.

  2009cites this paper
• Mechanisms of inhibition of zinc-finger transcription factors by selenium compounds ebselen and selenite.

  2009cites this paper
• Thyroid Autoimmune Antibodies For Enzyme Peroxidase, Thyroglobulin and Se, Zn Levels in Elderly Patients with Hypothyroidism in Iraq

  2009cites this paper
• Metal-coupled folding of Cys2His2 zinc-finger.

  2008cites this paper
• Le domaine THAP de THAP1 : structure par RMN en solution et interaction avec l'ADN

  2008influential citation
• New redesigned zinc-finger proteins: design strategy and its application.

  2008cites this paper
• Zinc binding of Tim10: Evidence for existence of an unstructured binding intermediate for a zinc finger protein

  2008cites this paper
• The regulation of RNA polymerase III transcription by protein kinase CK2

  2008cites this paper
• Acrodermatitis enteropathica and an overview of zinc metabolism.

  2007cites this paper
• Metal-Binding Domains in Nucleic Acid-Binding and Gene-Regulatory Proteins

  2007cites this paper
• Analysis of the structural consensus of the zinc coordination centers of metalloprotein structures.

  2007cites this paper
• Dual Role for Zn2+ in Maintaining Structural Integrity and Inducing DNA Sequence Specificity in a Promiscuous Endonuclease*

  2007cites this paper
• Serum Zinc Concentrations in Cystic Fibrosis Patients Aged Above 4 Years: A Cross-sectional Evaluation

  2007cites this paper
• Functional characterisation of three zinc transporters in Thlaspi caerulescens

  2007cites this paper
• All-electron calculations of the nucleation structures in metal-induced zinc-finger folding: role of the Peptide backbone.

  2007cites this paper
• Zinc fingers 1 and 7 of yeast TFIIIA are essential for assembly of a functional transcription complex on the 5 S RNA gene

  2007cites this paper
• Identification of transcriptional regulators for the Ustilago maydis mig genes

  2007cites this paper
• β‐Peptidic Secondary Structures Fortified and Enforced by Zn2+ Complexation – On the Way to β‐Peptidic Zinc Fingers?

  2006cites this paper
• Kinetic and structural studies of wild-type and recombinant aromatic amine dehydrogenase.

  2006cites this paper
• Functional characterization of iron-substituted tristetraprolin-2D (TTP-2D, NUP475-2D): RNA binding affinity and selectivity.

  2006cites this paper
• Zinc Coordination Environments in Proteins as Redox Sensors and Signal Transducers

  2006cites this paper
• Identification of a Zn2+-binding site on the dopamine D2 receptor.

  2006cites this paper
• Functional domains of the IS 1 transposase : analysis in vivo and in vitro

  2006influential citation
• La ribonucléotide réductase anaérobie d'Escherichia coli, une protéine à zinc : importance du site métallique pour la structure et l'activation de l'enzyme

  2006cites this paper
• Drosophila RNA binding proteins.

  2006cites this paper
• Chemistry and Biochemistry of Insulin-Mimetic Vanadium and Zinc Complexes. Trial for Treatment of Diabetes Mellitus

  2006cites this paper
• Nuclear translocation of alphaN-catenin by the novel zinc finger transcriptional repressor ZASC1.

  2005cites this paper
• Regulation of RNA polymerase III transcription during differentiation.

  2005cites this paper
• Transcriptome and proteome analysis identifies the pathways that increase hepatic lipid accumulation in zinc-deficient rats.

  2005cites this paper
• A comparison of the coordination geometries of some 4-methylimidazole-5-carbaldehyde complexes with Zn(II), Cd(II) and Co(II) ions in the solid state and aqueous solution

  2005cites this paper
• TFIIIA: A Sophisticated Zinc Finger Protein

  2005cites this paper
• C 2 H 2 Zinc Fingers As DNA Binding Domains

  2005cites this paper
• Zinc, oxidant-triggered cell signaling, and human health.

  2005cites this paper