Different Regulatory Mechanisms Modulate the Expression of a Dinoflagellate Iron-Superoxide Dismutase*

Regulation of antioxidant enzymes is critical to control the levels of reactive oxygen species in cell compartments highly susceptible to oxidative stress. In this work, we studied the regulation of a chloroplastic iron superoxide dismutase (Fe-SOD) fromLingulodinium polyedrum (formerly Gonyaulax polyedra) under different physiological conditions. A cDNA-encoding Fe-SOD was isolated from this dinoflagellate, showing high sequence similarity to cyanobacterial, algal, and plant Fe-SODs. Under standard growth conditions, on a 12:12-h light-dark cycle,Lingulodinium polyedrum Fe-SOD exhibited a daily rhythm of activity and cellular abundance with the maximum occurring during the middle of the light phase. Northern analyses showed that this rhythmicity is not related to changes in Fe-SOD mRNA levels, indicative of translational regulation. By contrast, conditions of metal-induced oxidative stress resulted in higher levels of Fe-SOD transcripts, suggesting that transcriptional control is responsible for increased protein and activity levels. Daily (circadian) and metal-induced up-regulation of Fe-SOD expression in L. polyedrum are thus mediated by different regulatory pathways, allowing biochemically distinct changes appropriate to oxidative challenges.

• Publication year

  2001

• Venue

  Journal of Biological Chemistry

• Publication date

  2001-06-08

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1074/jbc.M101169200PMID 11264289
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The structure and organization of the luciferase gene in the photosynthetic dinoflagellate Gonyaulax polyedra

  2004cited by this paper
• Antioxidant Modulation in Response to Metal-Induced Oxidative Stress in Algal Chloroplasts

  2001cited by this paper
• A region in the 3' UTR of MnSOD RNA enhances translation of a heterologous RNA.

  2000cited by this paper
• Identification of cis site involved in nickel-responsive transcriptional repression of sodF gene coding for Fe- and Zn-containing superoxide dismutase of Streptomyces griseus.

  2000cited by this paper
• Identification and Characterization of Three Differentially Expressed Genes, Encoding S-Adenosylhomocysteine Hydrolase, Methionine Aminopeptidase, and a Histone-Like Protein, in the Toxic Dinoflagellate Alexandrium fundyense

  2000cited by this paper
• Heavy metal-mediated activation of the rat Cu/Zn superoxide dismutase gene via a metal-responsive element

  1999cited by this paper
• The Activation of the Rat Copper/Zinc Superoxide Dismutase Gene by Hydrogen Peroxide through the Hydrogen Peroxide-responsive Element and by Paraquat and Heat Shock through the Same Heat Shock Element*

  1999cited by this paper
• Acute and chronic effects of toxic metals on viability, encystment and bioluminescence in the dinoflagellate Gonyaulax polyedra.

  1999cited by this paper
• Circadian synthesis of a nuclear-encoded chloroplast glyceraldehyde-3-phosphate dehydrogenase in the dinoflagellate Gonyaulax polyedra is translationally controlled.

  1999cited by this paper
• Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization.

  1998cited by this paper
• Response of superoxide dismutase to pollutant metal stress in the marine dinoflagellate Gonyaulax polyedra.

  1998cited by this paper
• The mRNA level of the circadian regulated Gonyaulax luciferase remains constant over the cycle.

  1998cited by this paper
• The 3' untranslated region of manganese superoxide dismutase RNA contains a translational enhancer element.

  1998cited by this paper
• Light-regulated transcription of genes encoding peridinin chlorophyll a proteins and the major intrinsic light-harvesting complex proteins in the dinoflagellate amphidinium carterae hulburt (Dinophycae). Changes In cytosine methylation accompany photoadaptation

  1998cited by this paper
• In vivo kinetics of a redox-regulated transcriptional switch.

  1997cited by this paper
• Superoxide Anion Radical (O·̄2), Superoxide Dismutases, and Related Matters*

  1997cited by this paper
• Redox regulation of NF-kappa B activation.

  1997cited by this paper
• The Manganese Superoxide Dismutase Gene of Drosophila: Structure, Expression, and Evidence for Regulation by MAP Kinase

  1997cited by this paper
• Oxidants as stimulators of signal transduction.

  1997cited by this paper
• In vivo kinetics of a redox-regulated transcriptional switch

  1997cited by this paper
• Oxidative damage and defense mechanisms in primary leaves of Phaseolus vulgaris as a result of root assimilation of toxic amounts of copper

  1996cited by this paper
• EFFECTS OF CADMIUM ON GROWTH AND SUPEROXIDE DISMUTASE ACTIVITY OF THE MARINE MICROALGA TETRASELMIS GRACILIS (PRASINOPHYCEAE) 1

  1996cited by this paper
• The effect of light on the biosynthesis of beta-carotene and superoxide dismutase activity in the photosynthetic alga Gonyaulax polyedra.

  1996cited by this paper
• Phased Protein Synthesis at Several Circadian Times Does Not Change Protein Levels in Gonyaulax

  1996cited by this paper
• Oxygen processing in photosynthesis.

  1996cited by this paper
• Isolation and Expression of Three Gibberellin 20-Oxidase cDNA Clones from Arabidopsis

  1995cited by this paper
• Dissection of Oxidative Stress Tolerance Using Transgenic Plants

  1995cited by this paper
• Oxidative mechanisms in the toxicity of metal ions.

  1995cited by this paper
• Reactive oxygen species as cellular messengers.

  1995cited by this paper
• Metal uptake of recombinant cambialistic superoxide dismutase from Propionibacterium shermanii is affected by growth conditions of host Escherichia coli cells.

  1995cited by this paper
• Circadian expression of the luciferin-binding protein correlates with the binding of a protein to the 3' untranslated region of its mRNA.

  1994cited by this paper
• The polypeptide components of scintillons, the bioluminescence organelles of the dinoflagellate Gonyaulax polyedra.

  1993cited by this paper
• SUPEROXIDE DISMUTASE AND STRESS TOLERANCE

  1992cited by this paper
• Too much of a good thing: light can be bad for photosynthesis.

  1992cited by this paper
• Glutathione Depletion Due to Copper-Induced Phytochelatin Synthesis Causes Oxidative Stress in Silene cucubalus.

  1992cited by this paper
• A circadian rhythm in the activity of superoxide dismutase in the photosynthetic alga Gonyaulax polyedra.

  1992cited by this paper
• Circadian control over synthesis of manyGonyaulax proteins is at a translational level

  1990cited by this paper
• Circadian regulation of bioluminescence in Gonyaulax involves translational control.

  1989cited by this paper
• Free Radicals in Biology and Medicine

  1986influential reference
• Free radicals in biology and medicine

  1985cited by this paper
• Superoxide‐dependent formation of hydroxyl radicals in the presence of iron salts

  1982cited by this paper
• Comparative aspects of basic chromatin proteins in dinoflagellates.

  1981cited by this paper
• Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

  1979cited by this paper
• Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.

  1971cited by this paper
• Algae: Amounts of DNA and Organic Carbon in Single Cells

  1969cited by this paper
• The reduction of cytochrome c by milk xanthine oxidase.

  1968cited by this paper
• Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran.

  1962cited by this paper

• Two chloroplast superoxide dismutase genes (MnSOD and CuZnSOD) in the freshwater dinoflagellate Palatinus apiculatus and their high-level gene expression in response to algicidal agents

  2025cites this paper
• The cryo-EM structure of Photosystem I from Chromera velia with a bound superoxide dismutase heterodimer

  2025cites this paper
• Potential siderophore-dependent mutualism in the harmful dinoflagellate Alexandrium pacificum (Group IV) and bacterium Photobacterium sp. TY1-4 under iron-limited conditions.

  2024cites this paper
• Antioxidant enzymes of Pseudochlorella pringsheimii under two stressors: variation of SOD Isoforms activity

  2023influential citation
• Heavy metal–induced stress in eukaryotic algae—mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response

  2022cites this paper
• The enigmatic clock of dinoflagellates, is it unique?

  2022cites this paper
• Two novel superoxide dismutase genes (CuZnSOD and MnSOD) in the toxic marine dinoflagellate Alexandrium pacificum and their differential responses to metal stressors.

  2022cites this paper
• Transcriptomic and metatranscriptomic approaches in phytoplankton: insights and advances

  2022cites this paper
• Dynamics of cell death across growth stages and the diel cycle in the dinoflagellate Karenia brevis

  2021cites this paper
• Species-specific oxidative stress responses and cellular energy allocation after coral shipping

  2021cites this paper
• Is It Good to Have a Stiff Aorta with Aging? Causes and Consequences.

  2021cites this paper
• Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

  2020cites this paper
• Molecular identification, differential expression and protective roles of iron/manganese superoxide dismutases in the green algae Closterium ehrenbergii against metal stress.

  2020cites this paper
• Cudratricusxanthone O Inhibits H2O2-Induced Cell Damage by Activating Nrf2/HO-1 Pathway in Human Chondrocytes

  2020cites this paper
• Impacts of ZnO nanoparticles on growth and antioxidant enzymes of the green alga Scenedesmus obliquus

  2020cites this paper
• Exogenous β-cyclocitral treatment primes tomato plants against drought by inducing tolerance traits, independent of abscisic acid.

  2020cites this paper
• Transcriptomic analysis reveals the roles of 2 detoxification systems in response to mercury in 3 Chromera velia 4

  2019cites this paper
• Green algal molecular responses to temperature stress

  2019cites this paper
• Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in Chromera velia

  2019cites this paper
• Molecular cloning and oxidative-stress responses of a novel manganese superoxide dismutase (MnSOD) gene in the dinoflagellate Prorocentrum minimum

  2019cites this paper
• The impact of thermal stress and nutrient availability on the physiology and proteome of symbiotic dinoflagellates

  2019cites this paper
• Diel transcriptional response of a California Current plankton microbiome to light, low iron, and enduring viral infection

  2019cites this paper
• Origin and roles of a novel copper-zinc superoxide dismutase (CuZnSOD) gene from the harmful dinoflagellate Prorocentrum minimum.

  2019cites this paper
• Oleic Acid Alleviates Cadmium-Induced Oxidative Damage in Rat by Its Radicals Scavenging Activity

  2018cites this paper
• Exploring dinoflagellate biology with high-throughput proteomics.

  2018cites this paper
• Mechanisms of Dysfunction in the Aging Vasculature and Role in Age-Related Disease

  2018cites this paper
• Neuregulin1 Attenuates H2O2-Induced Reductions in EAAC1 Protein Levels and Reduces H2O2-Induced Oxidative Stress

  2018cites this paper
• The Impact of Temperature Stress on DNA and RNA Synthesis in Potentially Toxic Dinoflagellates Prorocentrum minimum

  2018cites this paper
• Translation and Translational Control in Dinoflagellates

  2018cites this paper
• Cadmium induced oxidative stress and biochemical responses in cyanobacterium Nostoc muscorum

  2017cites this paper
• Garcinia xanthochymus extract protects PC12 cells from H2O2-induced apoptosis through modulation of PI3K/AKT and NRF2/HO-1 pathways.

  2017cites this paper
• Étude de la transcription par des techniques à haut-débit chez le dinoflagellé Lingulodinium polyedrum

  2017cites this paper
• Investigating physiological variability across different algal and cnidarian symbioses: Possible implications for climate change

  2016cites this paper
• The Mechanism of Diarrhetic Shellfish Poisoning Toxin Production in Prorocentrum spp.: Physiological and Molecular Perspectives

  2016cites this paper
• Dinoflagellate genomic organization and phylogenetic marker discovery utilizing deep sequencing data

  2016cites this paper
• Transcriptomic profiles reveal the genome-wide responses of the harmful dinoflagellate Cochlodinium polykrikoides when exposed to the algicide copper sulfate

  2016cites this paper
• Transcriptomic characterization of the enzymatic antioxidants FeSOD, MnSOD, APX and KatG in the dinoflagellate genus Symbiodinium

  2015cites this paper
• Sources of marine superoxide dismutases: Characteristics and applications.

  2015cites this paper
• Physiological response to elevated temperature and pCO2 varies across four Pacific coral species: Understanding the unique host+symbiont response

  2015cites this paper
• Melatonin regulates antioxidative mechanisms in microalgae Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)

  2015cites this paper
• Dinoflagellate Gene Structure and Intron Splice Sites in a Genomic Tandem Array

  2015influential citation
• Molecular cloning and functional characterization of MnSOD from Dunaliella salina

  2014cites this paper
• Cytoprotection of methionine on hyperthermia‐induced damage in bovine mammary epithelial cells

  2014cites this paper
• Transcription and Maturation of mRNA in Dinoflagellates

  2013cites this paper
• “Eco-omics”: A Review of the Application of Genomics, Transcriptomics, and Proteomics for the Study of the Ecology of Harmful Algae

  2013cites this paper
• Global Analysis of mRNA Half-Lives and de novo Transcription in a Dinoflagellate, Karenia brevis

  2013cites this paper
• Effect of temperature on oxidative stress in fish: Lipid peroxidation and catalase activity in the muscle of juvenile seabass, Dicentrarchus labrax

  2012cites this paper
• Transcriptional Response of Two Core Photosystem Genes in Symbiodinium spp. Exposed to Thermal Stress

  2012cites this paper
• Chromium (VI) induced oxidative stress in Hapalosiphon fontinalis

  2012cites this paper
• Two Fe-superoxide dismutase families respond differently to stress and senescence in legumes.

  2012cites this paper
• Coral Bleaching Resistance vs Susceptibility: The Role of Antioxidant Activity in Symbiotic Dinoflagellates

  2012cites this paper
• The influence of stress conditions on intracellular dimethylsulphoniopropionate (DMSP) and dimethylsulphide (DMS) release in Emiliania huxleyi

  2012cites this paper
• Expression of superoxide dismutase isoforms in Desmodesmus subspicatus cells exposed to anthropogenic contaminants

  2011cites this paper
• Physiological responses of Fucus serratus (Phaeophyceae) to high doses of cadmium exposure

  2011cites this paper
• Oxidative Stress in Tropical Marine Ecosystems

  2011cites this paper
• Recent progress in Symbiodinium transcriptomics

  2011cites this paper
• Differential Responses of the Coral Host and Their Algal Symbiont to Thermal Stress

  2011cites this paper
• RESPONSE OF ANTIOXIDANT SYSTEM IN SOME MARINE ALGAE UNDER CADMIUM STRESS

  2010cites this paper
• Cytoprotection of vitamin E on hyperthermia-induced damage in bovine mammary epithelial cells

  2010cites this paper
• Metal-specific and NADPH oxidase dependent changes in lipoxygenase and NADPH oxidase gene expression in Arabidopsis thaliana exposed to cadmium or excess copper.

  2010cites this paper
• Aspectos oxidativos e de biotransformação do poluente fenol na microalga Minutocellus polymorphus

  2009cites this paper
• Effect of Cu and Cd on Oxidative Enzymes and Chlorophyll Content of Moss Racomitrium crispulum

  2009cites this paper
• Physiological and biochemical responses to cadmium exposure in Fucus serratus (Phaeophyceae)

  2009cites this paper
• Cadmium-induced transcriptional and enzymatic alterations related to oxidative stress

  2008cites this paper
• Changes in electron transport, superoxide dismutase and ascorbate peroxidase isoenzymes in chloroplasts and mitochondria of cucumber leaves as influenced by chilling

  2008cites this paper
• Biochemical biomarkers in algae and marine pollution: a review.

  2008cites this paper
• Excess copper induces anoxygenic photosynthesis in Anabaena doliolum: a homology based proteomic assessment of its survival strategy

  2008cites this paper
• Molecular characterization of iron-containing superoxide dismutases in the heterotrophic dinoflagellate Crypthecodinium cohnii.

  2008cites this paper
• Molecular approaches to diagnosing nutritional physiology in harmful algae: Implications for studying the effects of eutrophication

  2008cites this paper
• Regulation of activity and gene expression of antioxidant enzymes in Ulva fasciata Delile (Ulvales, Chlorophyta) in response to excess copper

  2008cites this paper
• Regulation of activity and gene expression of antioxidant enzymes in Ulva fasciata Delile ( Ulvales , Chlorophyta ) in response to excess copper

  2008cites this paper
• Biodata of Shauna Murray, author of the chapter "Phylogenetics, Molecular Biology and Ecological Impacts of a Group of Highly Unusual Protists: The Dinoflagellates"

  2007cites this paper
• Toxic effects of anthraquinone and phenanthrenequinone upon Scenedesmus strains (green algae) at low and elevated concentration of CO2.

  2007cites this paper
• Phylogenetics, Molecular Biology and Ecological Impacts of a Group of Highly Unusual Protists

  2007cites this paper
• Spliced leader RNA trans-splicing in dinoflagellates

  2007cites this paper
• An unusual S-adenosylmethionine synthetase gene from dinoflagellate is methylated

  2007cites this paper
• Analysis of the targeting sequences of an iron-containing superoxide dismutase (SOD) of the dinoflagellate Lingulodinium polyedrum suggests function in multiple cellular compartments

  2007cites this paper
• MICROARRAY ANALYSIS OF DIURNAL‐ AND CIRCADIAN‐REGULATED GENES IN THE FLORIDA RED‐TIDE DINOFLAGELLATE KARENIA BREVIS (DINOPHYCEAE) 1

  2007cites this paper
• Circadian tempo: a paradigm for genome stability?

  2007cites this paper
• Global transcriptional profiling of the toxic dinoflagellate Alexandrium fundyense using Massively Parallel Signature Sequencing

  2006cites this paper
• Estrogenic and antioxidant effects of a phytoestrogen daidzein on ovarian germ cells in embryonic chickens.

  2006cites this paper
• Parallel analysis of transcript levels and physiological key parameters allows the identification of stress phase gene markers in Chlamydomonas reinhardtii under copper excess.

  2006cites this paper
• 1 STUDY ON THE EFFECT OF SOME SELECTED HEAVY METALS ON THE PRIMARY PRODUCTION IN FRESH WATER POND AT TIRUVANNAMALAI , TAMIL NADU

  2006cites this paper
• Life time—circadian clocks, mitochondria and metabolism

  2006cites this paper
• Localization and Role of Manganese Superoxide Dismutase in a Marine Diatom1[OA]

  2006cites this paper
• Oxidative stress in marine environments: biochemistry and physiological ecology.

  2006cites this paper
• Sesbania drummondii cell cultures: ICP-MS determination of the accumulation of Pb and Cu

  2005cites this paper
• Morphological Variation of Lingulodinium polyedrum (Dinophyceae) in Culture Specimens and Reinterpretation of the Thecal Formula

  2005cites this paper
• Rhythmicity and oxidative/nitrosative stress in algae

  2005cites this paper
• Biochemical characterization of a cambialistic superoxide dismutase isozyme from diatom Thallassiosira weissflogii: cloning, expression, and enzyme stability.

  2005cites this paper
• A novel rhythm of microcystin biosynthesis is described in the cyanobacterium Microcystis panniformis Komárek et al.

  2005cites this paper
• Circadian Rhythm of a TCA Cycle Enzyme Is Apparently Regulated at the Translational Level in the Dinoflagellate Lingulodinium polyedrum

  2005cites this paper
• Characterization of Fe/Mn-superoxide dismutase from diatom Thallassiosira weissflogii: cloning, expression, and property.

  2005cites this paper
• THE ROLE AND EVOLUTION OF SUPEROXIDE DISMUTASES IN ALGAE 1

  2005influential citation
• Identification of a mitochondrial superoxide dismutase with an unusual targeting sequence in Plasmodium falciparum.

  2004cites this paper
• Dinoflagellates: a remarkable evolutionary experiment.

  2004cites this paper
• Iron-superoxide dismutase and monodehydroascorbate reductase transcripts accumulate in response to internode rubbing in tomato.

  2004cites this paper
• Differential responses of stress proteins, antioxidant enzymes, and photosynthetic efficiency to physiological stresses in the Florida red tide dinoflagellate, Karenia brevis.

  2004cites this paper
• CIRCADIAN PROTECTION AGAINST OXIDATIVE STRESS IN MARINE ALGAE

  2004cites this paper
• Gene organization and homology modeling of two iron superoxide dismutases of the early branching protist Perkinsus marinus.

  2003cites this paper