Prognosis and Gene Expression Profiling of 20q13-Amplified Breast Cancers

Purpose: Amplification of chromosomal region 20q13 occurs in breast cancer but remains poorly characterized. Experimental Design: To establish the frequency of 20q13 amplification and select the amplified cases to be studied, we used fluorescence in situ hybridization of bacterial artificial chromosome probes for three 20q13 loci (MYBL2, STK6, ZNF217) on sections of tissue microarrays containing 466 primary carcinoma samples. We used Affymetryx whole-genome DNA microarrays to establish the gene expression profiles of 20q13-amplified tumors and quantitative reverse transcription-PCR to validate the results. Results: We found 36 (8%) 20q13-amplified samples. They were distributed in two types: type 1 tumors showed ZNF217 amplification only, whereas type 2 tumors showed amplification at two or three loci. Examination of the histoclinical features of the amplified tumors showed two strikingly opposite data. First, type 1 tumors were more frequently lymph node–negative tumors but were paradoxically associated with a poor prognosis. Second, type 2 tumors were more frequently lymph node–positive tumors but were paradoxically associated with a good prognosis. Type 1 and type 2 showed different gene expression profiles. No 20q13 gene could be associated with type 1 amplification, whereas several 20q13 genes were overexpressed in type 2 tumors. Conclusions: Our results suggest that amplified tumors of types 1 and 2 are two distinct entities resulting from two different mechanisms and associated to different prognosis.

• Publication year

  2006

• Venue

  Clinical Cancer Research

• Publication date

  2006-08-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1158/1078-0432.CCR-05-2339PMID 16899599
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Decoding the fine-scale structure of a breast cancer genome and transcriptome.

  2006cited by this paper
• Molecular evolution of breast cancer

  2005cited by this paper
• ZNF217 suppresses cell death associated with chemotherapy and telomere dysfunction.

  2005cited by this paper
• A 1 Mb minimal amplicon at 8p11–12 in breast cancer identifies new candidate oncogenes

  2005cited by this paper
• Protein expression profiling identifies subclasses of breast cancer and predicts prognosis.

  2005cited by this paper
• Comprehensive Profiling of 8p11-12 Amplification in Breast Cancer

  2005cited by this paper
• Visualizing chromosomes as transcriptome correlation maps: evidence of chromosomal domains containing co-expressed genes--a study of 130 invasive ductal breast carcinomas.

  2005influential reference
• NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis

  2005cited by this paper
• Aurora kinases, aneuploidy and cancer, a coincidence or a real link?

  2005cited by this paper
• Prognostic Relevance of Gene Amplifications and Coamplifications in Breast Cancer

  2004cited by this paper
• STK15/Aurora‐A expression in primary breast tumors is correlated with nuclear grade but not with prognosis

  2004cited by this paper
• The candidate oncogene ZNF217 is frequently amplified in colon cancer

  2004cited by this paper
• Preparation and characterization of a human aurora-A kinase monoclonal antibody

  2004cited by this paper
• A Recurrent Chromosome Breakpoint in Breast Cancer at the NRG1/Neuregulin 1/Heregulin Gene

  2004cited by this paper
• Identification and validation of an ERBB2 gene expression signature in breast cancers

  2004influential reference
• Prognostic relevance of 20q13 gains in sporadic colorectal cancers: a FISH analysis

  2004cited by this paper
• Split-signal FISH for detection of chromosome aberrations in acute lymphoblastic leukemia

  2004cited by this paper
• Genome Amplification of Chromosome 20 in Breast Cancer

  2003influential reference
• Characterization of the novel amplified in breast cancer-1 (NABC1) gene product.

  2003cited by this paper
• Exploration, normalization, and summaries of high density oligonucleotide array probe level data.

  2003cited by this paper
• A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples

  2003cited by this paper
• Repeated observation of breast tumor subtypes in independent gene expression data sets

  2003influential reference
• Determination of amplicon boundaries at 20q13.2 in tissue samples of human gastric adenocarcinomas by high‐resolution microarray comparative genomic hybridization

  2003cited by this paper
• Identification of TDE2 gene and its expression in non‐small cell lung cancer

  2003cited by this paper
• End-sequence profiling: Sequence-based analysis of aberrant genomes

  2003cited by this paper
• Distinct and complementary information provided by use of tissue and DNA microarrays in the study of breast tumor markers.

  2002cited by this paper
• Multiple genes in human 20q13 chromosomal region are involved in an advanced prostate cancer xenograft.

  2002cited by this paper
• Cloning of BCAS3 (17q23) and BCAS4 (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer †

  2002cited by this paper
• The ZNF 217 Gene Amplified in Breast Cancers Promotes Immortalization of Human Mammary Epithelial Cells 1

  2001cited by this paper
• The ZNF217 gene amplified in breast cancers promotes immortalization of human mammary epithelial cells.

  2001cited by this paper
• Comprehensive genome sequence analysis of a breast cancer amplicon.

  2001cited by this paper
• Comparative genomic hybridization analysis of 38 breast cancer cell lines: a basis for interpreting complementary DNA microarray data.

  2000cited by this paper
• Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene

  2000cited by this paper
• Frequent amplification of chromosomal region 20q12-q13 in ovarian cancer.

  2000cited by this paper
• Molecular portraits of human breast tumours

  2000influential reference
• Molecular classification of cancer: class discovery and class prediction by gene expression monitoring.

  1999cited by this paper
• Centrosomal kinase AIK1 is overexpressed in invasive ductal carcinoma of the breast.

  1999cited by this paper
• Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation

  1998cited by this paper
• Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma.

  1998cited by this paper
• Cluster analysis and display of genome-wide expression patterns.

  1998influential reference
• A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers

  1998cited by this paper
• A putative serine/threonine kinase encoding gene BTAK on chromosome 20q13 is amplified and overexpressed in human breast cancer cell lines

  1997cited by this paper
• Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer.

  1996influential reference
• The human CAS (cellular apoptosis susceptibility) gene mapping on chromosome 20q13 is amplified in BT474 breast cancer cells and part of aberrant chromosomes in breast and colon cancer cell lines.

  1996cited by this paper
• Amplification of chromosomal region 20q13 in invasive breast cancer: prognostic implications.

  1995influential reference
• Increased copy number at 20q13 in breast cancer: defining the critical region and exclusion of candidate genes.

  1994cited by this paper
• FGFRI and PLAT genes and DNA amplification at 8p 12 in breast and ovarian cancers

  1993cited by this paper

• Regulation of Apoptotic Pathways by MicroRNAs: A Therapeutic Strategy for Alzheimer’s Disease

  2025cites this paper
• Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolus

  2025cites this paper
• ZNF217 Gene Copy Number as a Marker of Response to Standard Therapy Drugs According to ERα Status in Breast Cancer

  2024cites this paper
• Genomic loci influence patterns of structural covariance in the human brain

  2023cites this paper
• Potential prognostic and predictive value of UBE2N, IMPDH1, DYNC1LI1 and HRASLS2 in colorectal cancer stool specimens

  2023cites this paper
• Identification of Two Genetic Loci Associated with Leukopenia after Chemotherapy in Breast Cancer Patients.

  2022cites this paper
• Novel genomic loci and pathways influence patterns of structural covariance in the human brain

  2022cites this paper
• Development of a Series of Neutralizing Nanobodies against SARS-CoV-2 Spike Protein

  2022cites this paper
• CEMIP as a potential biomarker and therapeutic target for breast cancer patients

  2022cites this paper
• The Intricate Interplay between the ZNF217 Oncogene and Epigenetic Processes Shapes Tumor Progression

  2022cites this paper
• FBXL2 promotes E47 protein instability to inhibit breast cancer stemness and paclitaxel resistance

  2022cites this paper
• Increased copy number of imprinted genes in the chromosomal region 20q11-q13.32 is associated with resistance to antitumor agents in cancer cell lines

  2022influential citation
• Bioinformatics analysis of the clinical significance of HLA class II in breast cancer

  2022cites this paper
• BDP1 Alterations Correlate with Clinical Outcomes in Breast Cancer

  2022cites this paper
• RNA-binding protein RNPC1 acts as an oncogene in gastric cancer by stabilizing Aurora kinase B mRNA.

  2021cites this paper
• ZNF217: the cerberus who fails to guard the gateway to lethal malignancy.

  2021cites this paper
• RNA Binding Protein RNPC1 Promotes Gastric Cancer Progression via Stabilizing the Aurora Kinase B mRNA

  2021cites this paper
• RNA-Binding Motif Protein 38 as a Potential Biomarker and Therapeutic Target in Cancer

  2020cites this paper
• RBM38 in cancer: role and mechanism

  2020cites this paper
• MYBL2 amplification in breast Cancer: Molecular mechanisms and therapeutic potential.

  2020cites this paper
• Differential expression, function and prognostic value of miR-17-92 cluster in ER-positive and triple-negative breast cancer.

  2020cites this paper
• RBM38 in cancer: role and mechanism

  2020cites this paper
• CC chemokines are differentially expressed in Breast Cancer and are associated with disparity in overall survival

  2019cites this paper
• Disruption of the Rbm38-eIF4E Complex with a Synthetic Peptide Pep8 Increases p53 Expression.

  2019cites this paper
• Association of PTPN1 polymorphisms with breast cancer risk: A case‐control study in Chinese females

  2019cites this paper
• Identification of aberrant gene expression during breast ductal carcinoma in situ progression to invasive ductal carcinoma

  2019cites this paper
• The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion

  2018cites this paper
• New statistical methodologies for improved analysis of genomic and omic data

  2018cites this paper
• B-Myb Mediates Proliferation and Migration of Non-Small-Cell Lung Cancer via Suppressing IGFBP3

  2018cites this paper
• A phase 1, first-in-human study of AMG 900, an orally administered pan-Aurora kinase inhibitor, in adult patients with advanced solid tumors

  2018cites this paper
• Improved estimation of SNP heritability using Bayesian multiple-phenotype models

  2017cites this paper
• MRL proteins cooperate with activated Ras in glia to drive distinct oncogenic outcomes

  2017cites this paper
• Overexpression of the E2F target gene CENPI promotes chromosome instability and predicts poor prognosis in estrogen receptor-positive breast cancer

  2017cites this paper
• The GDNF Family: A Role in Cancer?

  2017cites this paper
• Integrative genomic and transcriptomic analysis for pinpointing recurrent alterations of plant homeodomain genes and their clinical significance in breast cancer

  2016cites this paper
• Genomic similarity between gastroesophageal junction and esophageal Barrett's adenocarcinomas

  2016cites this paper
• Distinct lymphocyte antigens 6 (Ly6) family members Ly6D, Ly6E, Ly6K and Ly6H drive tumorigenesis and clinical outcome

  2016cites this paper
• RNA-binding protein RBM 38 : acting as a tumor suppressor in colorectal cancer

  2016cites this paper
• Biomarkers in ductal carcinoma in situ

  2016cites this paper
• Impact of non-neoplastic vs intratumoural hepatitis B viral DNA and replication on hepatocellular carcinoma recurrence

  2016influential citation
• Integrative genomic analyses of the RNA-binding protein, RNPC1, and its potential role in cancer prediction.

  2015cites this paper
• Intra-tumor genetic heterogeneity and alternative driver genetic alterations in breast cancers with heterogeneous HER2 gene amplification

  2015cites this paper
• Investigating the effects of increased levels of the translation elongation factor eEF1A within eukaryotic cells

  2015cites this paper
• Glycan‐related gene expression signatures in breast cancer subtypes; relation to survival

  2015cites this paper
• Studies on the role of two proteins in breast cancer - histone deacetylase 11 in estrogen receptor positive breast cancer and the redox protein memo in metastasis and tumorigenesis

  2015cites this paper
• The dark side of ZNF217, a key regulator of tumorigenesis with powerful biomarker value

  2015cites this paper
• Expression of Polarity Genes in Human Cancer

  2015cites this paper
• Spatiotemporal control of estrogen-responsive transcription in ERα-positive breast cancer cells

  2015cites this paper
• Melatonin Receptors and Their Preventive Role in Carcinogenesis

  2014cites this paper
• Loss of the Notch effector RBPJ promotes tumorigenesis

  2014cites this paper
• An epigenomic approach to therapy for tamoxifen-resistant breast cancer

  2014cites this paper
• Stromal and epithelial changes in breast cancer following endocrine treatment

  2014cites this paper
• Hypoxia-inducible factor 1 alpha is regulated by RBM38, a RNA-binding protein and a p53 family target, via mRNA translation

  2014cites this paper
• Prognostic significance of ZNF217 expression in gastric carcinoma.

  2014cites this paper
• RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer

  2014cites this paper
• The Rho GTPase Rnd1 suppresses mammary tumorigenesis and EMT by restraining Ras-MAPK signalling

  2014cites this paper
• Protein tyrosine phosphatases: structure, function, and implication in human disease.

  2013cites this paper
• Identification of ZNF217, hnRNP‐K, VEGF‐A and IPO7 as targets for microRNAs that are downregulated in prostate carcinoma

  2013cites this paper
• Amplification of distant estrogen response elements deregulates target genes associated with tamoxifen resistance in breast cancer.

  2013cites this paper
• Metastasis tumor-associated protein 2 enhances metastatic behavior and is associated with poor outcomes in estrogen receptor-negative breast cancer

  2013cites this paper
• Luminal breast cancer: from biology

  2013cites this paper
• Luminal breast cancer: from biology to treatment

  2013cites this paper
• Blockage of melatonin receptors impairs p53-mediated prevention of DNA damage accumulation.

  2013cites this paper
• Blockage of melatonin receptors impairs p53-mediated prevention of DNA damage accumulation

  2013cites this paper
• Phosphatase Modulators

  2013cites this paper
• Learning the local Bayesian network structure around the ZNF217 oncogene in breast tumours

  2013cites this paper
• GREB1 Functions as a Growth Promoter and Is Modulated by IL6/STAT3 in Breast Cancer

  2012cites this paper
• ZNF217 is a marker of poor prognosis in breast cancer that drives epithelial-mesenchymal transition and invasion.

  2012cites this paper
• SLC22A5/OCTN2 expression in breast cancer is induced by estrogen via a novel intronic estrogen-response element (ERE)

  2012cites this paper
• Regulation of p130(Cas)/BCAR1 expression in tamoxifen-sensitive and tamoxifen-resistant breast cancer cells by EGR1 and NAB2.

  2012cites this paper
• CAS protein expression in invasive breast ductal carcinoma and its relationship with cell proliferation and apoptosis: CAS protein expression in invasive breast ductal carcinoma and its relationship with cell proliferation and apoptosis

  2012cites this paper
• Interplay between AP-1 and estrogen receptor α in regulating gene expression and proliferation networks in breast cancer cells.

  2012cites this paper
• Engagement of I-branching {beta}-1, 6-N-acetylglucosaminyltransferase 2 in breast cancer metastasis and TGF-{beta} signaling.

  2011cites this paper
• Molecular differences between ductal carcinoma in situ and adjacent invasive breast carcinoma: a multiplex ligation-dependent probe amplification study

  2011cites this paper
• Molecular and Cellular Pathobiology Engagement of I-Branching b-1 , 6N-Acetylglucosaminyltransferase 2 in Breast Cancer Metastasis and TGF-b Signaling

  2011cites this paper
• Rôle de ZNF217, un nouvel oncogène dans le cancer du sein : rôle dans l’échappement tumoral et valeur pronostique

  2011cites this paper
• Artemin的研究进展

  2011cites this paper
• Phase 1 study of MLN8054, a selective inhibitor of Aurora A kinase in patients with advanced solid tumors

  2011cites this paper
• Single nucleotide polymorphisms in the 20q13 amplicon genes in relation to breast cancer risk and clinical outcome

  2011cites this paper
• Engagement of I-Branching b -1, 6- N -Acetylglucosaminyltransferase 2 in Breast Cancer Metastasis and TGF- b Signaling

  2011cites this paper
• Translational repression of p53 by RNPC1, a p53 target overexpressed in lymphomas.

  2011cites this paper
• PI-PLCβ1 gene copy number alterations in breast cancer.

  2011cites this paper
• Artemin: from neurotrophic factor to oncogene in human mammary carcinoma

  2011cites this paper
• Progesterone Receptor Inhibits Proliferation of Human Breast Cancer Cells via Induction of MAPK Phosphatase 1 (MKP-1/DUSP1)*

  2011cites this paper
• underlying breast cancer endocrine resistance cistrome α Growth factor stimulation induces a distinct ER Material

  2010cites this paper
• Soluble L1CAM promotes breast cancer cell adhesion and migration in vitro, but not invasion

  2010cites this paper
• Manipulating Protein Acetylation in Breast Cancer: A Promising Approach in Combination with Hormonal Therapies?

  2010cites this paper
• CXCR1 blockade selectively targets human breast cancer stem cells in vitro and in xenografts.

  2010cites this paper
• CCND1 and ZNF217 gene amplification is equally frequent in BRCA1 and BRCA2 associated and non-BRCA breast cancer.

  2010cites this paper
• Enumerating the gene sets in breast cancer, a "direct" alternative to hierarchical clustering

  2010cites this paper
• ZNF217 confers resistance to the pro-apoptotic signals of paclitaxel and aberrant expression of Aurora-A in breast cancer cells

  2010cites this paper
• Prognosis of hormone-dependent breast cancers: implications of the presence of dysfunctional transcriptional networks activated by insulin via the immune transcription factor T-bet.

  2010cites this paper
• The Proapoptotic Molecule BLID Interacts with Bcl-XL and Its Downregulation in Breast Cancer Correlates with Poor Disease-Free and Overall Survival

  2010cites this paper
• Addiction of MYCN Amplified Tumours to B-MYB Underscores a Reciprocal Regulatory Loop

  2010cites this paper
• Linking the septin expression with carcinogenesis

  2010influential citation
• Growth factor stimulation induces a distinct ER(alpha) cistrome underlying breast cancer endocrine resistance.

  2010cites this paper
• Molecular Differences between Ductal Carcinoma In Situ and Adjacent Invasive Breast Carcinoma: A Multiplex Ligation-Dependent Probe Amplification Study

  2010cites this paper
• Expression of Long-chain Fatty Acyl-CoA Synthetase 4 in Breast and Prostate Cancers Is Associated with Sex Steroid Hormone Receptor Negativity.

  2010cites this paper
• Trefoil factor 3 is oncogenic and mediates anti-estrogen resistance in human mammary carcinoma.

  2010cites this paper
• Molecular profiling of invasive breast cancer by multiplex ligation-dependent probe amplification-based copy number analysis of tumor suppressor and oncogenes

  2010cites this paper