Dermal Substitutes Support the Growth of Human Skin-Derived Mesenchymal Stromal Cells: Potential Tool for Skin Regeneration

New strategies for skin regeneration are needed in order to provide effective treatment for cutaneous wounds and disease. Mesenchymal stem cells (MSCs) are an attractive source of cells for tissue engineering because of their prolonged self-renewal capacity, multipotentiality, and ability to release active molecules important for tissue repair. In this paper, we show that human skin-derived mesenchymal stromal cells (SD-MSCs) display similar characteristics to the multipotent MSCs. We also evaluate their growth in a three-dimensional (3D) culture system with dermal substitutes (Integra and Pelnac). When cultured in monolayers, SD-MSCs expressed mesenchymal markers, such as CD105, Fibronectin, and α-SMA; and neural markers, such as Nestin and βIII-Tubulin; at transcriptional and/or protein level. Integra and Pelnac equally supported the adhesion, spread and growth of human SD-MSCs in 3D culture, maintaining the MSC characteristics and the expression of multilineage markers. Therefore, dermal substitutes support the growth of mesenchymal stromal cells from human skin, promising an effective tool for tissue engineering and regenerative technology.

• Publication year

  2014

• Venue

  PLoS ONE

• Publication date

  2014-02-26

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1371/journal.pone.0089542PMID 24586857PMCID 3935879
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Skeletal muscle pericyte subtypes differ in their differentiation potential.

  2013cited by this paper
• Fabrication, Characterization and Cellular Compatibility of Poly(Hydroxy Alkanoate) Composite Nanofibrous Scaffolds for Nerve Tissue Engineering

  2013cited by this paper
• Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation

  2013cited by this paper
• Differentiated markers in undifferentiated cells: Expression of smooth muscle contractile proteins in multipotent bone marrow mesenchymal stem cells

  2013cited by this paper
• Wound Repair and Regeneration

  2012cited by this paper
• Skin-derived multipotent stromal cells – an archrival for mesenchymal stem cells

  2012cited by this paper
• Paracrine Interactions between Mesenchymal Stem Cells Affect Substrate Driven Differentiation toward Tendon and Bone Phenotypes

  2012cited by this paper
• Dermal matrix scaffold engineered with adult mesenchymal stem cells and platelet‐rich plasma as a potential tool for tissue repair and regeneration

  2012cited by this paper
• Concise Review: Role of Mesenchymal Stem Cells in Wound Repair

  2012cited by this paper
• Perivascular cells for regenerative medicine

  2012cited by this paper
• Identification of class III β-tubulin as a marker of angiogenic perivascular cells.

  2012cited by this paper
• The elusive nature and function of mesenchymal stem cells

  2011cited by this paper
• Analysis of nuclear nestin localization in cell lines derived from neurogenic tumors

  2011influential reference
• Human Dermis Harbors Distinct Mesenchymal Stromal Cell Subsets

  2011cited by this paper
• The Role of Stem Cells in Cutaneous Wound Healing: What Do We Really Know?

  2011cited by this paper
• Reduced hydraulic permeability of three-dimensional collagen scaffolds attenuates gel contraction and promotes the growth and differentiation of mesenchymal stem cells.

  2010cited by this paper
• Cell delivery: intramyocardial injections or epicardial deposition? A head-to-head comparison.

  2009cited by this paper
• Local delivery of adipose-derived stem cells via acellular dermal matrix as a scaffold: a new promising strategy to accelerate wound healing.

  2009cited by this paper
• Chemically-Induced RAT Mesenchymal Stem Cells Adopt Molecular Properties of Neuronal-Like Cells but Do Not Have Basic Neuronal Functional Properties

  2009cited by this paper
• Skin tissue engineering

  2009cited by this paper
• Use of Human Mesenchymal Cells to Improve Vascularization in a Mouse Model for Scaffold-Based Dermal Regeneration

  2008cited by this paper
• A perivascular origin for mesenchymal stem cells in multiple human organs.

  2008cited by this paper
• In Search of the In Vivo Identity of Mesenchymal Stem Cells

  2008cited by this paper
• Engineered microenvironments for human stem cells.

  2008cited by this paper
• Human periodontal ligament: a niche of neural crest stem cells.

  2008cited by this paper
• Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.

  2006cited by this paper
• Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

  2006cited by this paper
• Gene expression perturbation in vitro--a growing case for three-dimensional (3D) culture systems.

  2005cited by this paper
• Molecular regulation of vascular smooth muscle cell differentiation in development and disease.

  2004cited by this paper
• Nestin expression – a property of multi-lineage progenitor cells?

  2004cited by this paper
• Class III β-Tubulin Isotype: A Key Cytoskeletal Protein at the Crossroads of Developmental Neurobiology and Tumor Neuropathology

  2003cited by this paper
• Multilineage potential of adult human mesenchymal stem cells.

  1999cited by this paper
• Pericytes and periendothelial cells of brain parenchyma vessels co‐express aminopeptidase N, aminopeptidase A, and nestin

  1999cited by this paper
• Further applications of "bilayer artificial skin".

  1995cited by this paper
• Wound repair and regeneration

  1994cited by this paper
• Successful Use of a Physiologically Acceptable Artificial Skin in the Treatment of Extensive Burn Injury

  1981cited by this paper

• Comparing mesenchymal stem/stromal cells from dermis discarded in elective surgeries: Identifying optimal sources for regenerative medicine.

  2026cites this paper
• A Bioabsorbable Implant Seeded with Adipose-Derived Stem Cells for Adipose Regeneration

  2025influential citation
• Artificial dermis combined with autologous razor-thin skin graft for repair of cervical hidradenitis suppurativa: A case report

  2025cites this paper
• Injectable hydrogel harnessing foreskin mesenchymal stem cell-derived extracellular vesicles for treatment of chronic diabetic skin wounds.

  2024cites this paper
• Application of mesenchymal stem/stromal cells in periodontal regeneration: Opportunities and challenges

  2024cites this paper
• Objective Non-Invasive Bio-Parametric Evaluation of Regenerated Skin: A Comparison of Two Acellular Dermal Substitutes

  2024cites this paper
• Stem Cell Therapies and Ageing: Unlocking the Potential of Regenerative Medicine.

  2024cites this paper
• Poly-L-lactic acid in the correction of age-related changes in facial skin.

  2024cites this paper
• Mesenchymal stem cells and natural killer cells interaction mechanisms and potential clinical applications

  2022cites this paper
• Comparison of Wound Healing Effect of Skin Micrograft Impregnated into Two Kinds of Artificial Dermis in a Murine Wound Model

  2022cites this paper
• Development of gelatin hydrogel nonwoven fabrics (Genocel®) as a novel skin substitute in murine skin defects

  2022cites this paper
• Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing

  2022cites this paper
• Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

  2021cites this paper
• Human adipose-derived mesenchymal stromal cells from face and abdomen undergo replicative senescence and loss of genetic integrity after long-term culture

  2021cites this paper
• Mesenchymal stromal cells from dermal and adipose tissues induce macrophage polarization to a pro-repair phenotype and improve skin wound healing.

  2020cites this paper
• Utility of a New Artificial Dermis as a Successful Tool in Face and Scalp Reconstruction for Skin Cancer: Analysis of the Efficacy, Safety, and Aesthetic Outcomes

  2020cites this paper
• Combination product of dermal matrix, human mesenchymal stem cells, and timolol promotes diabetic wound healing in mice

  2020cites this paper
• CCID_A_266015 701..710

  2020cites this paper
• Indeterminate-Depth Burn Injury-Exploring the Uncertainty.

  2020cites this paper
• Effect of Hyaluronic Acid and Poly-L-Lactic Acid Dermal Fillers on Collagen Synthesis: An in vitro and in vivo Study

  2020cites this paper
• Decellularized extracellular matrix bioinks and their application in skin tissue engineering

  2020cites this paper
• Proangiogenic peptide nanofiber hydrogel/3D printed scaffold for dermal regeneration

  2020cites this paper
• [Masquelet technique combined with artificial dermis for the treatment of bone and soft tissue defects in rabbits].

  2019cites this paper
• Skin Epidermis and Adnexa Regrowth Induced by Treatment With Artificial Dermal Template After Full-Thickness Skin Wound

  2019cites this paper
• In vitro comparative study of human mesenchymal stromal cells from dermis and adipose tissue for application in skin wound healing

  2019cites this paper
• Treatment of Large Scars in Children Using Artificial Dermis and Scalp Skin Grafting

  2019cites this paper
• The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells

  2019cites this paper
• Notch Signaling in Nestin‐Expressing Cells in the Bone Marrow Maintains Erythropoiesis via Macrophage Integrity

  2019cites this paper
• Title A Comparison of Conventional Collagen Sponge andCollagen-Gelatin Sponge in Wound Healing

  2019cites this paper
• The Role of Autologous Dermal Micrografts in Regenerative Surgery: A Clinical Experimental Study

  2019cites this paper
• Isolating stem cells from skin: designing a novel highly efficient non-enzymatic approach.

  2019cites this paper
• Carrageenan hydrogel as a scaffold for skin-derived multipotent stromal cells delivery

  2018cites this paper
• 3D bio-printing technology for body tissues and organs regeneration

  2018cites this paper
• Dermal Regeneration and Induction of Wound Closure in Diabetic Wounds

  2018cites this paper
• Perinatal regenerative medicine : development of autologous constructs for soft tissue defects

  2018cites this paper
• Mesenchymal Stem Cells for the Treatment of Skin Diseases

  2017cites this paper
• Transplantation of Human Skin-Derived Mesenchymal Stromal Cells Improves Locomotor Recovery After Spinal Cord Injury in Rats

  2017cites this paper
• Properties of skin stem cells and their potential clinical applications in modern dermatology

  2017cites this paper
• Recent progress in the study of skin mesenchymal stem cells

  2017cites this paper
• 3D Printing of Scaffold for Cells Delivery: Advances in Skin Tissue Engineering

  2016influential citation
• Bilaminar Device of Poly(Lactic-co-Glycolic Acid)/Collagen Cultured With Adipose-Derived Stem Cells for Dermal Regeneration.

  2016cites this paper
• A Comparison of Conventional Collagen Sponge and Collagen-Gelatin Sponge in Wound Healing

  2016cites this paper
• Migration and Proliferative Activity of Mesenchymal Stem Cells in 3D Polylactide Scaffolds Depends on Cell Seeding Technique and Collagen Modification

  2016cites this paper
• Adult Stem Cell Therapies for Wound Healing: Biomaterials and Computational Models

  2016cites this paper
• The Development of Tissue Engineering Skin

  2015cites this paper
• Comparative Experimental Study of Wound Healing in Mice: Pelnac versus Integra

  2015cites this paper
• Skin equivalents: skin from reconstructions as models to study skin development and diseases

  2015cites this paper
• Quercetin and Rutin Affect the Survival and Proliferation of Human Skin-Derived Multipotent Mesenchymal Stromal Cells

  2015cites this paper
• UNIVERSIDADE FEDERAL DE SANTA CATARINA CENTRO DE CIÊNCIAS BIOLÓGICAS PROGRAMA DE PÓS-GRADUAÇÃO EM BIOLOGIA CELULAR E DO DESENVOLVIMENTO

  2014cites this paper