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Quantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftments

W. Ye,Zhiwu Jiang,Guan-xiong Li,Yiren Xiao,Simiao Lin,Y. Lai,Suna Wang,Baiheng Li,B. Jia,Yin Li,Zhi-liang Huang,Jin Li,F. Feng,Shuhua Li,H. Yao,Zixia Liu,S. Cao,Ling Xu,Yangqiu Li,Donghai Wu,Lingwen Zeng,M. Zhong,Pentao Liu,Z. Wen,Bing Xu,Yao Yao,D. Pei,Peng Li

Published 2015 in Journal of Hematology & Oncology

ABSTRACT

BackgroundThe mouse is an organism that is widely used as a mammalian model for studying human physiology or disease, and the development of immunodeficient mice has provided a valuable tool for basic and applied human disease research. Following the development of large-scale mouse knockout programs and genome-editing tools, it has become increasingly efficient to generate genetically modified mouse strains with immunodeficiency. However, due to the lack of a standardized system for evaluating the immuno-capacity that prevents tumor progression in mice, an objective choice of the appropriate immunodeficient mouse strains to be used for tumor engrafting experiments is difficult.MethodsIn this study, we developed a tumor engraftment index (TEI) to quantify the immunodeficiency response to hematologic malignant cells and solid tumor cells of six immunodeficient mouse strains and C57BL/6 wild-type mouse (WT).ResultsMice with a more severely impaired immune system attained a higher TEI score. We then validated that the NOD-scid-IL2Rg−/− (NSI) mice, which had the highest TEI score, were more suitable for xenograft and allograft experiments using multiple functional assays.ConclusionsThe TEI score was effectively able to reflect the immunodeficiency of a mouse strain.

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