The use of the carbon/nitrogen ratio and specific organic loading rate as tools for improving biohydrogen production in fixed-bed reactors☆

M. Anzola-Rojas,Samantha Gonçalves da Fonseca,Cynthia Canêdo da Silva,Valéria Maia de Oliveira,M. Zaiat

Published 2014 in Biotechnology Reports

ABSTRACT

Highlights • The effects of the carbon/nitrogen ratios of 40, 90, 140, and 190 on hydrogen production are evaluated by varying the nitrogen source in an upflow fixed-bed anaerobic reactor.• An optimal C/N ratio of 137 to produce 3.5 mol H2 mol−1 sucrose is estimated by a mathematical approximation.• Continuous decreases in the specific organic loading rate as a function of time seemed to be responsible for the instability of the system.• A microbial biology analysis identified hydrogen-producing and -consuming microorganisms from natural inoculum.

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