Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  2011-05-18

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1073/pnas.1105687108PMID 21593407PMCID PMC3111295
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• X-ray-induced deterioration of disulfide bridges at atomic resolution.

  2010cited by this paper
• Fluorescence Detection of Heavy Atom Labeling (FD-HAL): a rapid method for identifying covalently modified cysteine residues by phasing atoms.

  2010cited by this paper
• Structure and Function of an Irreversible Agonist-β2 Adrenoceptor complex

  2010cited by this paper
• Ins and outs of major facilitator superfamily antiporters.

  2008cited by this paper
• The Crystal Structure of a Sodium Galactose Transporter Reveals Mechanistic Insights into Na+/Sugar Symport

  2008influential reference
• The Cys154-->Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway.

  2008cited by this paper
• Sugar binding in lactose permease: anomeric state of a disaccharide influences binding structure.

  2007cited by this paper
• Sugar binding induces an outward facing conformation of LacY

  2007cited by this paper
• Structural determination of wild-type lactose permease

  2007cited by this paper
• Single-molecule FRET reveals sugar-induced conformational dynamics in LacY

  2007cited by this paper
• Site-directed alkylation and the alternating access model for LacY

  2007cited by this paper
• Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY

  2006influential reference
• Lessons from lactose permease.

  2006influential reference
• Sugar Recognition by the Lactose Permease of Escherichia coli*

  2004cited by this paper
• Identification of common structural features of binding sites in galactose‐specific proteins

  2004influential reference
• Monitoring Conformational Rearrangements in the Substrate-binding Site of a Membrane Transport Protein by Mass Spectrometry*

  2004cited by this paper
• Coot: model-building tools for molecular graphics.

  2004cited by this paper
• Aromatic stacking in the sugar binding site of the lactose permease.

  2003cited by this paper
• Probing the Mechanism of a Membrane Transport Protein with Affinity Inactivators*

  2003influential reference
• Structure and Mechanism of the Lactose Permease of Escherichia coli

  2003influential reference
• Changing the lactose permease of Escherichia coli into a galactose-specific symporter

  2002influential reference
• The PyMOL Molecular Graphics System

  2002cited by this paper
• Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helices IV and V that contain the major determinants for substrate binding.

  2001cited by this paper
• The kamikaze approach to membrane transport

  2001cited by this paper
• The C-4 hydroxyl group of galactopyranosides is the major determinant for ligand recognition by the lactose permease of Escherichia coli.

  2001influential reference
• Bioorganic Chemistry: Carbohydrates. Edited by Sidney M. Hecht, 1998, First Edition, Oxford University Press, ISBN 0-19-508469-1, 640 pages, Hardback, $79.95

  1999cited by this paper
• The major facilitator superfamily.

  1999cited by this paper
• Cys‐scanning mutagenesis: a novel approach to structure—function relationships in polytopic membrane proteins

  1998cited by this paper
• The substrate-binding site in the lactose permease of Escherichia coli.

  1998cited by this paper
• Preservation and staining of myelinated nerve fibers.

  1998cited by this paper
• Substituted-cysteine accessibility method.

  1998cited by this paper
• Macromolecular crystallography. Part A. Methods in Enzymology, Vol. 276. Edited by Charles W. Carter Jr and Robert M. Sweet. New York: Academic Press, 1997. Pp. xxxii + 700. Price $99.00. ISBN 0-12-182177-3

  1998cited by this paper
• Lactose binding to heat-labile enterotoxin revealed by X-ray crystallography

  1992cited by this paper
• Construction of a functional lactose permease devoid of cysteine residues.

  1991cited by this paper
• Transport Studies in Bacterial Membrane Vesicles

  1974cited by this paper
• Transport Studies in Bacterial Membrane Vesicles

  1974cited by this paper
• Mechanisms of active transport in isolated bacterial membrane vesicles. 18. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone.

  1974cited by this paper
• Mechanisms of active transport in isolated bacterial membrane vesicles. Further studies on amino acid transport in Staphylococcus aureus membrane vesicles.

  1972cited by this paper
• Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli.

  1971cited by this paper
• Mechanisms of active transport in isolated membrane vesicles. 2. The coupling of reduced phenazine methosulfate to the concentrative uptake of beta-galactosides and amino acids.

  1971cited by this paper
• Electronic Reprint Biological Crystallography Likelihood-enhanced Fast Translation Functions Biological Crystallography Likelihood-enhanced Fast Translation Functions

  year unknowncited by this paper
• Electronic Reprint Biological Crystallography Phenix : a Comprehensive Python-based System for Macromolecular Structure Solution

  year unknowncited by this paper

• Trimbody with rigid AI-designed scaffolds enables atomic-resolution cryo-EM structure determination of small proteins.

  2026cites this paper
• Maltose and Maltotriose Transporters in Brewer’s Saccharomyces Yeasts: Polymorphic and Key Residues in Their Activity

  2025cites this paper
• Flipping the switch: dynamic modulation of membrane transporter activity in bacteria

  2023cites this paper
• The non-Newtonian behavior of detergents during concentration is increased by macromolecules, in trans, and results in their over-concentration.

  2022cites this paper
• It's Better To Be Lucky Than Smart.

  2021cites this paper
• DKG-PIPD: A Novel Method About Building Deep Knowledge Graph

  2021cites this paper
• Investigation of sugar binding kinetics of the E. coli sugar/H+ symporter XylE using solid-supported membrane-based electrophysiology

  2021cites this paper
• Energy Metabolism | Glucose/Sugar Transport in Bacteria

  2021cites this paper
• Structural and evolutionary analyses of the Plasmodium falciparum chloroquine resistance transporter

  2020cites this paper
• Diversity in kinetics correlated with structure in nano body-stabilized LacY

  2020cites this paper
• Structural and functional adaptability of sucrose and lactose permeases from Escherichia coli to the membrane lipid composition.

  2020cites this paper
• Crystallization and preliminary X-ray diffraction studies of melibiose permease

  2020cites this paper
• The lipid-dependent structure and function of LacY can be recapitulated and analyzed in phospholipid-containing detergent micelles

  2019cites this paper
• It takes two to tango: The dance of the permease

  2019influential citation
• Lactose Permease: From Membrane to Molecule to Mechanism

  2019cites this paper
• The proton electrochemical gradient induces a kinetic asymmetry in the symport cycle of LacY

  2019cites this paper
• Novel NMR Avenues to Explore the Conformation and Interactions of Glycans

  2019cites this paper
• Secondary Active Transporters.

  2019cites this paper
• Key amino acid residues of the AGT1 permease required for maltotriose consumption and fermentation by Saccharomyces cerevisiae

  2018influential citation
• Engineered occluded apo-intermediate of LacY

  2018cites this paper
• Molecular Basis of Substrate Polyspecificity of the Candida albicans Mdr1p Multidrug/H+ Antiporter.

  2018cites this paper
• Oversized galactosides as a probe for conformational dynamics in LacY

  2018cites this paper
• Crystal Structure of a ligand-bound LacY–Nanobody Complex

  2018cites this paper
• Uptake dynamics in the Lactose permease (LacY) membrane protein transporter

  2018cites this paper
• Exploration of conformational changes in lactose permease upon sugar binding and proton transfer through coarse‐grained simulations

  2017cites this paper
• pKa of Glu 325 in LacY

  2017cites this paper
• Improving Fine-Grained Resource Mapping on Tightly Coupled Heterogeneous Multi-cores

  2017influential citation
• Structural biology of solute carrier (SLC) membrane transport proteins

  2017cites this paper
• pKa of Glu325 in LacY

  2017cites this paper
• X-ray diffraction reveals the intrinsic difference in the physical properties of membrane and soluble proteins

  2017cites this paper
• Quantification of Detergents Complexed with Membrane Proteins

  2017cites this paper
• Soft Matter in Lipid-Protein Interactions.

  2017cites this paper
• Chapter 2 Functional Properties of Organic Cation Transporter OCT 1 , Binding of Substrates and Inhibitors , and Presumed Transport Mechanism

  2017cites this paper
• An Asymmetric Conformational Change in LacY.

  2017cites this paper
• Insights into Protein–Ligand Interactions: Mechanisms, Models, and Methods

  2016cites this paper
• Crystal structure of a LacY–nanobody complex in a periplasmic-open conformation

  2016cites this paper
• Coarse‐grained simulations of proton‐dependent conformational changes in lactose permease

  2016cites this paper
• Functional Properties of Organic Cation Transporter OCT1, Binding of Substrates and Inhibitors, and Presumed Transport Mechanism

  2016cites this paper
• Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY.

  2016cites this paper
• Title Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY Permalink

  2016cites this paper
• Understanding transport by the major facilitator superfamily (MFS): structures pave the way

  2016cites this paper
• Thermodynamics of Nanobody Binding to Lactose Permease.

  2016cites this paper
• Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: The "Mix-and-Match" Method.

  2015cites this paper
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  2015cites this paper
• Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site

  2015cites this paper
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  2015cites this paper
• Works Title Thermodynamic mechanism for inhibition of lactose permease by the phosphotransferase protein

  2015cites this paper
• Principal Component Analysis reveals correlation of cavities evolution and functional motions in proteins.

  2015cites this paper
• Transport of sugars.

  2015cites this paper
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  2015cites this paper
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  2015cites this paper
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  2015cites this paper
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  2015cites this paper
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  2015cites this paper
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  2015cites this paper
• Thermodynamic mechanism for inhibition of lactose permease by the phosphotransferase protein IIAGlc

  2015cites this paper
• Structure and function of SemiSWEET and SWEET sugar transporters.

  2015cites this paper
• Escherichia coli as host for membrane protein structure determination: a global analysis

  2014cites this paper
• Flexible Gates Generate Occluded Intermediates in the Transport Cycle of LacY

  2014cites this paper
• Evolutionary mix-and-match with MFS transporters II.

  2014cites this paper
• When two turn into one: evolution of membrane transporters from half modules

  2014cites this paper
• Structure of sugar-bound LacY

  2014cites this paper
• Real-time conformational changes in LacY

  2014cites this paper
• Insights into the Inhibitory Mechanisms of the Regulatory Protein IIAGlc on Melibiose Permease Activity*

  2014cites this paper
• Outward-facing conformers of LacY stabilized by nanobodies

  2014cites this paper
• Title Evolutionary mix-and-match with MFS transporters II Permalink

  2014cites this paper
• Expression, Purification and Crystallization Studies of Lactose Permease Mutants

  2014cites this paper
• Functional architecture of MFS d-glucose transporters

  2014cites this paper
• The Life and Times of Lac Permease: Crystals Ain’t Everything, but They Certainly Do Help

  2014cites this paper
• Function, Structure, and Evolution of the Major Facilitator Superfamily: The LacY Manifesto

  2014cites this paper
• Substrate-induced changes in the structural properties of LacY

  2014cites this paper
• permease activity on melibiose Glc the regulatory protein IIA Insights into the inhibitory mechanisms of

  2014cites this paper
• Structure of the Trehalose-6-phosphate Phosphatase from Brugia malayi Reveals Key Design Principles for Anthelmintic Drugs

  2014cites this paper
• Galactoside-Binding Site in LacY

  2014cites this paper
• Electrophysiological characterization of uncoupled mutants of LacY.

  2013cites this paper
• Trp replacements for tightly interacting Gly–Gly pairs in LacY stabilize an outward-facing conformation

  2013cites this paper
• The periplasmic cavity of LacY mutant Cys154→Gly: how open is open?

  2013cites this paper
• Evolutionary mix-and-match with MFS transporters II

  2013cites this paper
• Structural advances for the major facilitator superfamily (MFS) transporters.

  2013cites this paper
• Evolutionary mix-and-match with MFS transporters

  2013cites this paper
• Using Cys-Scanning Analysis Data in the Study of Membrane Transport Proteins

  2013cites this paper
• Análise estrutural da permease Agt1p de Saccharomyces cerevisiae

  2012cites this paper
• Phosphatidylethanolamine-lactose permease interaction: a comparative study based on FRET.

  2012cites this paper
• Alternating access mechanism in the POT family of oligopeptide transporters

  2012cites this paper
• Evidence for an intermediate conformational state of

  2012cites this paper
• Apo-intermediate in the transport cycle of lactose permease (LacY)

  2012cites this paper
• Curvature forces in membrane lipid-protein interactions.

  2012cites this paper
• Curvature Forces in Membrane Lipid − Protein Interactions

  2012cites this paper
• Dynamics of the l-fucose/H+ symporter revealed by fluorescence spectroscopy

  2012cites this paper
• Nitrate transport and assimilation in Aspergillus nidulans

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  2012cites this paper
• Substitution of transmembrane domain Cys residues alters pHo-sensitive anion transport by AE2/SLC4A2 anion exchanger

  2012cites this paper
• Evidence for an intermediate conformational state of LacY

  2012cites this paper
• Role of the irreplaceable residues in the LacY alternating access mechanism

  2012cites this paper
• Proton-coupled dynamics in lactose permease.

  2012cites this paper
• Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation.

  2012cites this paper
• Lactose permease and the alternating access mechanism.

  2011cites this paper
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  year unknowncites this paper