Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  2011-06-15

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1073/pnas.1100125108PMID 21676862PMCID PMC3158158
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Arp2/3 complex is bound and activated by two WASP proteins

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• Nucleotide- and activator-dependent structural and dynamic changes of arp2/3 complex monitored by hydrogen/deuterium exchange and mass spectrometry.

  2009influential reference
• The structural basis of actin filament branching by the Arp2/3 complex

  2008influential reference
• Structure and Biochemical Properties of Fission Yeast Arp2/3 Complex Lacking the Arp2 Subunit*

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• Pathway of Actin Filament Branch Formation by Arp2/3 Complex*

  2008influential reference
• Close Packing of Listeria monocytogenes ActA, a Natively Unfolded Protein, Enhances F-actin Assembly without Dimerization*

  2008cited by this paper
• X-ray scattering study of activated Arp2/3 complex with bound actin-WCA.

  2008influential reference
• Hierarchical regulation of WASP/WAVE proteins.

  2008influential reference
• SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis.

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• Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex.

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• Regulation of actin filament assembly by Arp2/3 complex and formins.

  2007influential reference
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• Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP.

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  2003influential reference
• Interaction of cortactin and N-WASp with Arp2/3 complex.

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  1998cited by this paper
• The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments.

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• Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization.

  1996cited by this paper
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  1984cited by this paper
• Identification of a factor in conventional muscle actin preparations which inhibits actin filament self-association.

  1980cited by this paper

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