Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass.

No abstract is available for this paper.

• Publication year

  2010

• Venue

  Plant Biotechnology Journal

• Publication date

  2010-04-01

• Fields of study

  Biology, Medicine, Engineering, Environmental Science

• Identifiers
  DOI 10.1111/j.1467-7652.2009.00458.xPMID 20051035
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2009influential reference
• A high-oleic-acid and low-palmitic-acid soybean: agronomic performance and evaluation as a feedstock for biodiesel.

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  2008cited by this paper
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  2008influential reference
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  2008influential reference
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  2007influential reference
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  2007influential reference
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  2006cited by this paper
• Bio-ethanol--the fuel of tomorrow from the residues of today.

  2006cited by this paper
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  1983influential reference
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