Corticotropin-releasing factor receptors and actions in rat Leydig cells.

Rat Leydig cells possess functional high affinity receptors for corticotropin-releasing factor (CRF). CRF inhibited human chorionic gonadotropin (hCG)-induced androgen production in cultured fetal and adult Leydig cells in a dose-dependent manner, but it had no effect on basal testosterone secretion. Comparable inhibitory effects of CRF were observed in the presence or absence of 3-isobutyl-1-methylxanthine. CRF treatment caused a marked reduction of steroid precursors of the androgen pathway (from pregnenolone to testosterone) during gonadotropin stimulation, but it did not influence their basal levels. The inhibitory action of CRF on hCG-induced steroidogenesis was fully reversed by 8-bromo-cAMP but was not affected by pertussis toxin. The action of CRF was rapid; and it was blocked by coincubation with anti-CRF antibody. CRF caused no changes in hCG binding to Leydig cells, and in contrast to other target tissues, CRF did not stimulate cAMP production, indicating that CRF receptors are not coupled to Gs in Leydig cells. These studies have demonstrated that CRF-induced inhibition of the acute steroidogenic action of hCG is exerted at sites related to receptor/cyclase coupling or cAMP formation. The inhibitory effects of CRF in the Leydig cell do not occur through the Gi unit of adenylate cyclase, but could involve pertussis toxin-insensitive G protein(s). These observations demonstrate that CRF has a novel and potent antireproductive effect at the testicular level. Since CRF is synthesized in the testis and is present in Leydig cells, it is likely that locally produced CRF could exert negative autocrine modulation on the stimulatory action of luteinizing hormone on Leydig cell function.

• Publication year

  1989

• Venue

  Journal of Biological Chemistry

• Publication date

  1989-02-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)94155-4PMID 2464587
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Corticotropin-releasing factor receptors in mouse spleen: identification, autoradiographic localization, and regulation by divalent cations and guanine nucleotides.

  1988cited by this paper
• Angiotensin II receptors and inhibitory actions in Leydig cells.

  1988cited by this paper
• Beta-endorphin production by the fetal Leydig cell: regulation and implications for paracrine control of Sertoli cell function.

  1988cited by this paper
• Evidence for involvement of guanine nucleotide‐binding regulatory proteins in the activation of phospholipases by hormones

  1988cited by this paper
• Presence of immunoreactive corticotropin-releasing factor in rat testis.

  1988cited by this paper
• Corticotropin-releasing factor in the dog adrenal medulla is secreted in response to hemorrhage.

  1987cited by this paper
• Evidence for local stimulation of ACTH secretion by corticotropin-releasing factor in human placenta

  1987cited by this paper
• Corticotropin-releasing factor decreases plasma luteinizing hormone levels in female rats by inhibiting gonadotropin-releasing hormone release into hypophysial-portal circulation.

  1987cited by this paper
• Corticotropin-releasing factor receptors in the rat central nervous system: characterization and regional distribution

  1987cited by this paper
• Stimulatory and inhibitory effects of protein kinase C activation and calcium ionophore on cultured pig Leydig cells.

  1987cited by this paper
• Control of aldosterone production by angiotensin II is mediated by two guanine nucleotide regulatory proteins.

  1987cited by this paper
• Calcium-dependent control of corticotropin release in rat anterior pituitary cell cultures.

  1987cited by this paper
• Molecular mechanisms of corticotropin-releasing factor stimulation of calcium mobilization and adrenocorticotropin release from anterior pituitary tumor cells.

  1987cited by this paper
• Rat corticotropin-releasing hormone gene: sequence and tissue-specific expression.

  1987cited by this paper
• Stress-induced inhibition of reproductive functions: role of endogenous corticotropin-releasing factor.

  1986cited by this paper
• Involvement of protein kinase C in the regulation of adrenocorticotropin release from rat anterior pituitary cells.

  1986cited by this paper
• Acquisition of estradiol-mediated regulatory mechanism of steroidogenesis in cultured fetal rat Leydig cells.

  1986cited by this paper
• Corticotropin-releasing factor binding to the anterior pituitary receptor is modulated by divalent cations and guanyl nucleotides.

  1986cited by this paper
• gamma-Endorphin-generating endopeptidase: distribution in body tissues and cellular localization in rat testis.

  1986cited by this paper
• Functional corticotropin releasing factor receptors in the primate peripheral sympathetic nervous system

  1986cited by this paper
• Regulation of corticotropin-releasing factor (CRF) receptors in the rat pituitary gland: effects of adrenalectomy on CRF receptors and corticotroph responses.

  1985cited by this paper
• Phorbol ester causes desensitization of gonadotropin-responsive adenylate cyclase in a murine Leydig tumor cell line.

  1985cited by this paper
• Fetal Leydig cell culture--an in vitro system for the study of trophic hormone and GnRH receptors and actions.

  1985cited by this paper
• Opiate receptors are present in the rat testis. Identification and localization in Sertoli cells.

  1985cited by this paper
• Mitochondrial cholesterol availability during gonadotropin-induced Leydig cell desensitization.

  1985cited by this paper
• Distribution of corticotropin releasing factor-like immunoreactivity in the rat brain by immunohistochemistry and radioimmunoassay: comparison and characterization of ovine and rat/human CRF antisera.

  1985cited by this paper
• Role of Ni in coupling angiotensin receptors to inhibition of adenylate cyclase in hepatocytes.

  1985cited by this paper
• Direct stimulation by thyrotropin-releasing hormone (TRH) of polyphosphoinositide hydrolysis in GH3 cell membranes by a guanine nucleotide-modulated mechanism.

  1985cited by this paper
• Inhibition of hCG‐stimulated adenylate cyclase in purified mouse Leydig cells by the phorbol ester PMA

  1985cited by this paper
• Phospholipid methylation by intact rat Leydig cells.

  1985cited by this paper
• Autoradiographic localization of angiotensin II receptors in rat brain.

  1984cited by this paper
• Distribution and characterization of immunoreactive corticotropin-releasing factor in human tissues.

  1984cited by this paper
• Functional and morphological studies on isolated Leydig cells: purification by centrifugal elutriation and metrizamide fractionation.

  1984cited by this paper
• Brain and pituitary receptors for corticotropin releasing factor: localization and differential regulation after adrenalectomy.

  1984cited by this paper
• Beta-endorphin and adrenocorticotropin release from rat adenohypophysis in vitro: evidence for local modulation by arachidonic acid metabolites of the cyclooxygenase and lipoxygenase pathway.

  1984cited by this paper
• Receptors and inhibitory actions of gonadotropin-releasing hormone in the fetal Leydig cell.

  1984cited by this paper
• Phorbol esters inhibit LH stimulated steroidogenesis by mouse Leydig cells in vitro.

  1984cited by this paper
• Synthetic competitive antagonists of corticotropin-releasing factor: effect on ACTH secretion in the rat.

  1984cited by this paper
• Studies on the Identification of a LHRH‐like Peptide in the Rat Testis

  1984cited by this paper
• Corticotropin releasing factor stimulation of protein carboxylmethylation in mouse pituitary tumor cells.

  1983cited by this paper
• Corticotropin-releasing factor is a potent inhibitor of sexual receptivity in the female rat

  1983cited by this paper
• Organization of ovine corticotropin-releasing factor immunoreactive cells and fibers in the rat brain: an immunohistochemical study.

  1983cited by this paper
• Mechanisms of action of corticotropin-releasing factor and other regulators of corticotropin release in rat pituitary cells.

  1983cited by this paper
• Corticotropin-releasing factor stimulates phospholipid methylation and corticotropin secretion in mouse pituitary tumor cells.

  1982cited by this paper
• Corticotropin-releasing factor immunoreactivity in human gastrointestinal tract.

  1982cited by this paper
• Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin.

  1982cited by this paper
• Ligand: a versatile computerized approach for characterization of ligand-binding systems.

  1980cited by this paper
• Regulation of luteinizing hormone receptors and steroidogenesis in gonadotropin-desensitized leydig cells.

  1978cited by this paper
• Intermediate role of adenosine 3':5'-cyclic monophosphate and protein kinase during gonadotropin-induced steroidogenesis in testicular interstitial cells.

  1977cited by this paper
• THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

  1963cited by this paper

• Characterization of Corticotropin-releasing Hormone Receptors (CRHRs) and Binding Proteins (CRHBPs) in Nile Tilapia: Molecular identification, functional analysis and tissue distribution

  2024cites this paper
• New Insights Into the Evolution of Corticotropin-Releasing Hormone Family With a Special Focus on Teleosts

  2022cites this paper
• Urocortin stimulates ERK1/2 phosphorylation and proliferation but reduces ATP production of MCF7 breast cancer cells.

  2022cites this paper
• Neuropeptides as regulators of the hypothalamus-pituitary-gonadal (HPG) axis activity and their putative roles in stress-induced fertility disorders.

  2021cites this paper
• Corticotropin-releasing hormone reduces basal estradiol production in zebrafish follicular cells.

  2021cites this paper
• Metabolic Association between Leptin and the Corticotropin Releasing Hormone

  2019cites this paper
• Corticotropin-Releasing Hormone (CRH)

  2019cites this paper
• The Corticotropin-Releasing Factor Family: Physiology of the Stress Response.

  2018cites this paper
• Local Actions of Melatonin in Somatic Cells of the Testis

  2017cites this paper
• CRH Receptor Signalling: Potential Roles in Pathophysiology.

  2017cites this paper
• MiR-375 Mediates CRH Signaling Pathway in Inhibiting E2 Synthesis in Porcine Ovary.

  2016cites this paper
• Acute Stress, Hypothalamic-Hypophyseal-Gonadal Axis and Testicular Function – A Review

  2015cites this paper
• Neonatal maternal separation in mice as a pre-clinical model for female chronic pelvic pain

  2015cites this paper
• Restraint Stress Impairs Oocyte Developmental Potential in Mice: Role of CRH-Induced Apoptosis of Ovarian Cells1

  2013cites this paper
• Insights into mechanisms of corticotropin‐releasing hormone receptor signal transduction

  2012cites this paper
• A Transcriptome-Wide Screen for mRNAs Enriched in Fetal Leydig Cells: CRHR1 Agonism Stimulates Rat and Mouse Fetal Testis Steroidogenesis

  2012cites this paper
• Is There a Link between Exercise and Male Factor Infertility

  2011cites this paper
• Urocortins are present in the rat testis.

  2011cites this paper
• Regulation of the hypothalamic-pituitary-adrenal axis by neuropeptides

  2011cites this paper
• Regulation of Male Fertility by the Opioid System

  2011cites this paper
• Effect of intratesticular administration of anti‐corticotropin‐releasing factor antiserum (a‐CRF) on testicular function in neonatal rats

  2009cites this paper
• Urocortin 1 inhibits rat leydig cell function.

  2008cites this paper
• Expression of leptin and leptin receptor in the testis of fertile and infertile patients

  2007cites this paper
• Separate Locations of Urocortin and its Receptors in Mouse Testis: Function in Male Reproduction and the Relevant Mechanisms

  2007cites this paper
• Corticotropin releasing hormone: a diagnostic marker for behavioral and reproductive disorders?

  2007cites this paper
• CHAPTER 48 – Stress and the Reproductive System

  2006cites this paper
• Exercise and the stress system.

  2005cites this paper
• Expression of human prepro-orexin and signaling characteristics of orexin receptors in the male reproductive system.

  2004cites this paper
• The hypothalamic-pituitary-adrenal (HPA) axis: A major mediator of the adaptive responses to stress

  2003cites this paper
• The emerging role of peripheral corticotropin-releasing hormone (CRH)

  2003cites this paper
• Maternal and Fetal Hypothalamic‐Pituitary‐Adrenal Axes During Pregnancy and Postpartum

  2003cites this paper
• Ontogenesis of Leptin Receptor in Rat Leydig Cells1

  2003cites this paper
• Importance of the paraventricular nucleus of the hypothalamus as a component of a neural pathway between the brain and the testes that modulates testosterone secretion independently of the pituitary.

  2003influential citation
• Interactions between Testicular Serotoninergic, Catecholaminergic, and Corticotropin-Releasing Hormone Systems Modulating cAMP and Testosterone Production in the Golden Hamster

  2002cites this paper
• Inhibitory Effect of Neurogenic and Immune Stressors on Testosterone Secretion in Rats

  2002cites this paper
• Endocrinology of Complex Life Cycles: Amphibians

  2002cites this paper
• Role of corticotropin-releasing hormone in ovarian steroidogenesis

  2002cites this paper
• Corticotrophin-releasing hormone inhibits insulin-like growth factor-I release from primary cultures of rat granulosa cells.

  2002cites this paper
• Metyrapone-induced corticosterone deficiency impairs glucose oxidation and steroidogenesis in Leydig cells of adult albino rats.

  2002cites this paper
• Effects of electro-acupuncture on corticotropin-releasing factor in rats with experimentally-induced polycystic ovaries.

  2001cites this paper
• Corticotropin‐releasing hormone binding to the syncytiotrophoblast membranes

  2001cites this paper
• Leptin modulates the corticotropin-releasing hormone (CRH) action on progesterone release from cultured rat granulosa cells.

  2000cites this paper
• Signal transduction characteristics of the corticotropin-releasing hormone receptors in the feto-placental unit.

  2000influential citation
• Maternal Hypothalamic‐Pituitary‐Adrenal Axis in Pregnancy and the Postpartum Period: Postpartum‐Related Disorders

  2000cites this paper
• Stress during pregnancy alters the offspring hypothalamic, pituitary, adrenal, and testicular response to isolation on the day of weaning.

  1999cites this paper
• Expression of functional leptin receptors in rodent Leydig cells.

  1999cites this paper
• Expression of Functional Leptin Receptors in Rodent Leydig Cells1.

  1999cites this paper
• Changes in the hormonal concentrations of pregnant rats and their fetuses following multiple exposures to a stressor during the third trimester.

  1999cites this paper
• Corticotropin-releasing factor inhibits luteinizing hormone-stimulated P450c17 gene expression and androgen production by isolated thecal cells of human ovarian follicles.

  1998cites this paper
• Mechanisms of Stress on Reproduction: Evidence for a Complex Intra‐Hypothalamic Circuit

  1998cites this paper
• The degradation of corticotropin-releasing factor by enzymes of the rat brain studied by liquid chromatography-mass spectrometry.

  1998cites this paper
• Corticotropin-Releasing Factor (CRF) Agonists Stimulate Testosterone Production in Mouse Leydig Cells through CRF Receptor-1.

  1998cites this paper
• The cellular mechanisms of corticotropin-releasing hormone (CRH)-stimulated steroidogenesis in mouse Leydig cells are similar to those for LH.

  1997cites this paper
• Corticotropin-releasing hormone stimulates the expression of the steroidogenic acute regulatory protein in MA-10 mouse cells.

  1997cites this paper
• Transport of CRH from mouse brain directly affects peripheral production of beta-endorphin by the spleen.

  1997cites this paper
• The Hypothalamic‐Pituitary‐Thyroid Axis and the Female Reproductive System

  1997cites this paper
• Expression of genes encoding corticotropin-releasing factor (CRF), type 1 CRF receptor, and CRF-binding protein and localization of the gene products in the human ovary.

  1997cites this paper
• Autocrine/Paracrine Intermediates in Hormonal Action and Modulation of Cellular Responses to Hormones

  1997cites this paper
• Transport of CRH from mouse brain directly affects peripheral production of β-endorphin by the spleen.

  1997cites this paper
• Gonadal peptides as mediators of development and functional control of the testis: an integrated system with hormones and local environment.

  1997influential citation
• Autocrine—Paracrine Role of Ovarian Corticotropin‐Releasing Hormone

  1997cites this paper
• The Hypothalamic‐Pituitary‐Adrenal Axis and the Female Reproductive System

  1997cites this paper
• Corticotropin-releasing hormone (CRH) inhibits steroid biosynthesis by cultured human granulosa-lutein cells in a CRH and interleukin-1 receptor-mediated fashion.

  1997cites this paper
• Alcohol stimulates ACTH secretion in the rat: mechanisms of action and interactions with other stimuli.

  1996cites this paper
• Splenic macrophages can modify steroidogenesis of Leydig cells.

  1996cites this paper
• Effects of corticotropin-releasing hormone on ovarian estrogen production in vitro.

  1996cites this paper
• Mechanism of the Stress‐Induced Attenuation of the Testicular Response to Gonadotropin: Possible Involvement of Testicular Opioids, a Pertussis Toxin‐Sensitive G‐Protein, and Phosphodiesterase

  1996cites this paper
• Role of P2-purinergic receptors in rat Leydig cell steroidogenesis.

  1996cites this paper
• The Genomic Structure of the Rat Corticotropin Releasing Factor Receptor

  1996cites this paper
• Effect of corticotropin-releasing factor on the pituitary-ovary axis in human luteal phase.

  1995cites this paper
• Aging and acute stress decrease corticotropin releasing hormone in the ovary of the Fischer 344/N rat.

  1995cites this paper
• Corticotropin-releasing hormone stimulates steroidogenesis in mouse Leydig cells.

  1995cites this paper
• The human myometrium expresses multiple isoforms of the corticotropin-releasing hormone receptor.

  1995cites this paper
• Calcium signaling in single rat Leydig cells.

  1995cites this paper
• Identification and characterization of a corticotrophin-releasing hormone receptor in human placenta.

  1995cites this paper
• Direct effects of the pineal hormone melatonin on testosterone synthesis of Leydig cells in Djungarian hamsters (Phodopus sungorus) in vitro.

  1995cites this paper
• Immune corticotropin-releasing hormone is present in the eyes of and promotes experimental autoimmune uveoretinitis in rodents.

  1995cites this paper
• Corticotropin releasing factor decidualizes human endometrial stromal cells in vitro. Interaction with progestin.

  1995cites this paper
• Corticotropin-releasing factor (CRF) and stress-related reproductive failure: The brain as a state of the art or the ovary as a novel clue?

  1995cites this paper
• CRF administered to pregnant rats alters offspring behavior and morphology.

  1995cites this paper
• Stress-induced genetic expression of a selective corticotropin-releasing factor-receptor subtype within the rat ovaries: an effect dependent on the ovulatory cycle.

  1995cites this paper
• Sexual dimorphism of stress response and immune/ inflammatory reaction: the corticotropin releasing hormone perspective

  1995cites this paper
• Temperature effect on the action of corticotropin-releasing hormone (CRH) on secretion of immunoreactive beta-endorphin (IR beta EP) in TM3 and AtT-20 cell lines.

  1994cites this paper
• Presence of immunoreactive corticotropin-releasing hormone in normal and polycystic human ovaries.

  1994cites this paper
• Leydig cells: endocrine, paracrine, and autocrine regulation.

  1994cites this paper
• Identification of thyrotropin-releasing hormone receptor in the rat testis.

  1994cites this paper
• Hormonal regulation of human corticotropin-releasing hormone gene expression: implications for the stress response and immune/inflammatory reaction.

  1994cites this paper
• Presence of immunoreactive corticotropin releasing hormone in thyroid lesions.

  1994cites this paper
• Régulation paracrine des fonctions leydigiennes

  1993cites this paper
• Immunoreactive corticotropin-releasing hormone and its binding sites in the rat ovary.

  1993cites this paper
• Corticotropin-releasing factor mRNA in rat thymus and spleen.

  1993cites this paper
• Corticotropin‐Releasing Hormone and Its Receptors in the Ovary: Physiological Implications

  1993cites this paper
• Corticotropin‐releasing factor: an antireproductive hormone of the testis

  1993cites this paper
• Serotonergic inhibition of rat Leydig cell function by propranolol.

  1993cites this paper
• Nonsteroidal signals originating in the gonads.

  1992cites this paper
• Local regulation of Leydig cell function by inhibitors of steroidogenic activity.

  1992cites this paper
• Evidence for specific binding and stimulatory effects of recombinant human erythropoietin on isolated adult rat Leydig cells.

  1992cites this paper
• Regulation of corticotropin-releasing factor secretion from Leydig cells by serotonin.

  1992cites this paper
• Growth hormone-releasing hormone is produced by rat Leydig cell in culture and acts as a positive regulator of Leydig cell function.

  1992cites this paper
• Fetal Leydig Cells: Cellular Origin, Morphology, Life Span, and Special Functional Features

  1992cites this paper